Ersin FADILOĞLU, Hüseyin ÖZYURT, Hasan ERDOĞAN, Mehmet Hanifi EMRE

L-Name ile hipertansif yapılan sıçanlarda kalpte iskemi-reperfüzyon sonrası kalp dokusu ksantin oksidaz aktivitesi ve malondialdehit düzeyleri

Bu çalışmanın amacı erkek ve dişi hipertansif sıçanlarda kalpte iskemi-reperfüzyon hasarından sonra ksantin oksidaz (XO) aktivitesinin ve malondialdehit (MDA) düzeyinin tespit saptanmasıdır. L-NAME ile kronik nitrik oksit sentetaz (NOS) inhibisyonu hipertansiyon modeli oluşturmak için uygulandı. Erkek ve dişi sıçanlar üçer gruba ayrıldı. L-NAME çeşme suyu içinde 15 gün süre ile 100 mg/L ya da 500 mg/L olacak şekilde verildi. Kontrol grubuna sadece çeşme suyu verildi, iskemi-reperfüzyon (l/R) hasarı oluşturmak için sol ana koroner artere 7 dakika oklüzyon ve 7 dakika reperfüzyon uygulandı. Sonra kalp çıkarıldı ve -85'C'de çalışma gününe kadar saklandı. Dişi kontrol grubu XO aktivitesi erkek gruba göre anlamlı olarak artmış bulundu. Dişi sıçanlarda 100 mg L-NAME grubu XO aktivitesi kontrol grubuna göre anlamlı olarak azaldı. Erkek 100 mg L-NAME grubu MDA düzeyi diğer erkek gruplarına göre anlamlı artış gösterdi, üiş ikontrol grubu MDA düzeyi diğer dişi çalışma gruplarına göre vè ayrıca 500 mg L-NAME grubu MDA düzeyi 100 mg L-NAME grubuna göre anlamlı o/arak azalmış olarak saptandı. Düşük doz L-NAME ile hipertansiyon oluşturulan erkek sıçanlarda kalpte l/R sonrası lipit peroksidasyonda artış görülürken, diğer taraftan L-NAME ile hipertansiyon oluşturulan dişi sıçanlarda kalpte l/R sonrası lipit peroksidasyonda azalma görüldü. L-NAME ile hipertansiyon oluşturulan dişi sıçanlarda I/R sonrası XO enzim aktivitesi anlamlı bir şekilde azalmıştır.

Anahtar Kelimeler:

Lipid peroksidasyonu, Hipertansiyon, Sıçanlar, Modeller, hayvan, Malondialdehit, Ksantin oksidaz, Kalp, Reperfüzyon hasarı

Xhanthine oxidase activities and malondialdehyde levels in heart tissue after myocardial ischemia-reperfusion on rats with hypertension induced by L-Name

The aim of this study was to investigate xanthine oxidase (OX)'enzyme activity and level of malondialdehyde (MDA) following heart ischemia-reperfüsion injury in male and female hypertensive rats. Chronic nitric oxide synthetase inhibition by L-NAME was performed to induce hypertension. Male and female rats were divided into three groups. L-NAME was given as 100 mg/L or 500 mg/L in tap water for 15 days. Only tap water was given to rats in control group. To produce ischemia-reperfüsion (I/R) injury, the left main coronary artery was occluded for 7 mm, followed by 7 min of reperfusion. Then heart was excised and had been stored at -85'C up to study day. XO activity of female control group was higher than male one. It was found that XO activity of female 100 mg L-NAME group was lower than control one. MDA level of male 100 mg L-NAME group showed remarkable increase compared to other male group. It was found that MDA level of female control group was higher than those of other female groups. On the other hand, MDA level of female 500 mg L-NAME group was significantly lower than 100 mg L-NAME one. Increased lipid peroxidatlon .was seen after myocardial I-R injury in male rats with hypertension induced by low dose L-NAME, whereas decreased lipid peroxidation was seen after myocardial I-R injury in female rats with hypertension induced by L-NAME. Hypertension induced, by low dose L-NAME caused remarkable decrease in XO enzyme activity of female rats after I/R.

Keywords:

Lipid Peroxidation, Hypertension, Rats, Models, Animal, Malondialdehyde, Xanthine Oxidase, Heart, Reperfusion Injury,

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