Ece Cansu OKUR, Mehmet Fatih ORHAN, Bahri ELMAS

Vitamin B12 eksikliği olan çocuklarda tiyol disülfit dengesi

Amaç: Bu çalışmada, vitamin B12 eksikliği olan çocuklarda oksidatif stres belirteci olarak tiyol/disülfit dengesinin araştırılması amaçlanmıştır. Gereç ve Yöntem: Çalışmaya vitamin B12 eksikliği olan 61 çocuk hasta ve 62 sağlıklı çocuk kontrol grubu olarak dahil edildi. Katılımcıların vitamin B12, homosistein, tam kan sayımı ve tiyol/disülfit dengesi parametreleri ölçüldü. Grupların klinik ve laboratuvar parametreleri karşılaştırıldı. Tiyol/disülfit dengesi parametreleri ile vitamin B12 ve homosistein arasındaki ilişki araştırıldı. Bulgular: Gruplar yaş ve cinsiyet dağılımı açısından benzerdi. Medyan vitamin B12 düzeyi hasta grubunda 179 (98-199) pg/ml, kontrol grubunda 298 (201-965) pg/ml idi. Hasta ve kontrol grubu arasında; homosistein, nativ tiyol, total tiyol, disülfit, disülfit/nativ tiyol oranı, disülfit/total tiyol oranı ve nativ tiyol/total tiyol oranı açısından anlamlı fark saptanmadı. Hasta ve kontrol grubunda, tiyol/disülfit dengesi parametreleri ile vitamin B12 ve homosistein düzeyleri arasında anlamlı korelasyon saptanmadı. Sonuç: Hasta grubumuzda vitamin B12 düzeyinin belirgin düşük olmaması nedeniyle homosistein düzeyinde anlamlı artış olmadığı düşünülmüştür. Homosisteini artırmayan bir vitamin B12 eksikliğinin, oksidatif stresi artırmayacağı, dolayısıyla antioksidan kapasiteyi de artırmayacağı öngörülebilir.

Anahtar Kelimeler:

Çocuk, Vitamin B12, Homosistein, Oksidatif stres, Tiyol/disülfit dengesi

Thiol disulfide balance in children with vitamin B12 deficiency

Purpose: The aim of this study was to investigate the thiol/disulfide balance as a marker of oxidative stress in children with vitamin B12 deficiency. Materials and Methods: Sixty one pediatric patient with vitamin B12 deficiency and 62 healthy children as a control group was included in the study. Vitamin B12, homocysteine, complete blood count, and thiol/disulfide balance parameters of the participants were measured. The relationship between thiol/disulfide balance parameters and vitamin B12 and homocysteine was investigated. Results: The groups were similar in terms of age and gender distribution. Median vitamin B12 level was 179 (98-199) pg/ml in the patient group and 298 (201-965) pg/ml in the control group. Median homocysteine level was 11.2 (0.08-64.3) µmol/L in the patient group and 12 (4.5-26.6) µmol/L in the control group. Median values of homocysteine levels were within the normal range in both groups. Between the patient and control groups; no significant difference was found in terms of homocysteine, native thiol, total thiol, disulfide, disulfide/native thiol ratio, disulfide/total thiol ratio and native thiol/total thiol ratio. There was no significant correlation between thiol/disulfide balance parameters and vitamin B12 and homocysteine levels in the patient and control groups. Conclusion: There was no significant increase in homocysteine level, since the vitamin B12 level was not significantly low in our patient group. It can be predicted that a vitamin B12 deficiency that does not increase homocysteine will not increase oxidative stress and therefore will not increase antioxidant capacity.

Keywords:

Child, Homocysteine, Vitamin B12, Oxidative stress, Thiol/disulphide balance,

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