Cenk EKMEKCİ, Sümeyye EKMEKCİ, Cem YÜCEL, Ebru ÇAKIR

Ratlarda cisplatin kaynaklı kardiyotoksisite üzerine all-trans retinoik asidin kardiyoprotektif etkisi

Amaç: Cisplatin, pek çok farklı tümör tedavisinde kullanılan etkili bir antineoplastik ajandır. Retinoik asit güçlü antioksidan etkilere sahiptir. Bu çalışmanın amacı, all-trans retinoik asidin (ATRA) cisplatin kaynaklı kardiyotoksisite üzerine etkilerini araştırmaktır. Gereç ve Yöntem: Çalışmada wistar albino ratlar kullanıldı. Kontrol grubundaki ratlara 10 gün boyunca günlük 1 ml/kg salin intraperitoneal (i.p.) olarak verildi ve ATRA grubundaki ratlara 10 gün boyunca tek doz (7,5 mg/kg/gün) ATRA i.p. verildi. ATRA + CP grubundaki ratlara 10 gün boyunca tek doz (7,5 mg/kg/gün) ATRA i.p. ve tedavinin dördüncü gününde tek doz CP (7mg/kg) i.p. verildi. CP grubundaki ratlara ise 10 günlük tedavinin dördüncü gününde tek doz CP (7mg/kg) i.p. verildi. Tedaviden sonra gruplar kardiyak histopatoloji bulgularına göre karşılaştırıldı. Bulgular: Nekroz, sitoplazmik vakuolizasyon, konjesyon, kanama ve ödem cisplatin grubunda kontrol grubuna göre daha yaygındı. All-trans retinoik asid + cisplatin grubunda nekroz ve sitoplazmik vakuolizasyon, CP grubundan istatistiksel olarak anlamlı olarak daha az gözlendi. Sonuç: Bu çalışma cisplatin tedavisinin kalp dokusu üzerinde yıkıcı etkileri olduğunu doğruladı ve all-trans retinoik asid tedavisinin histopatolojik olarak cisplatine bağlı kardiyotoksisiteyi önleyebildiğini gösterdi.

Anahtar Kelimeler:

Cisplatin, kardiotoksisite, retinoik asid, all-trans retinoik asid

Cardioprotective effect of all-trans retinoic acid on cisplatin induced cardiotoxicity in rats

used in the treatment of various tumors. Retinoic acid has potent antioxidant effects. In this study, it is aimed to reveal the effects of all-trans retinoic acid (ATRA) on CP induced cardiotoxicity. Materials and Methods: In the study, wistar albino rats were used. Control group received single daily doses of 1 ml/kg saline and ATRA group received single daily doses of ATRA(7,5mg/kg/day) intraperitoneally (i.p.) for 10 days. ATRA+CP group received a single dose of CP(7mg/kg) i.p. on the fourth day of the 10 days of ATRA (7,5mg/kg/day) i.p. treatment. The rats in the CP group received only a single dose of cisplatin(7mg/kg) i.p. given on the 4th of 10 days of treatment. After treatment, the groups were compared based on cardiac histopathology findings. Results: Necrosis, cytoplasmic vacuolization, congestion, hemorrhage and edema were more common in the CP group than the control group). Necrosis and cytoplasmic vacuolization in the all-trans retinoic acid + cisplatin group was observed statistically significantly less frequently than the CP group. Conclusion: This study confirmed that cisplatin therapy had destructive effects on heart tissue, and showed that all-trans retinoic acid treatment could histopathologically prevent cisplatin-induced cardiotoxicity.

Keywords:

Cisplatin, cardiotoxicity, retinoic acid, all-trans retinoic acid,

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