Erken Gebelikte Diyabet, Sıçanların Endometrial Bezlerinin Karbonhidrat Salgılarını Etkiler mi?

Amaç: Endometriyal bez salgıları uterus alıcılığında önemli bir rol oynar ve implantasyondan önce embriyonun beslenmesini sağlar. Diyabetin belirli hücrelerin salgılarını değiştirdiği bilinmektedir. Bu çalışmada, Fıstık Aglutinin (PNA), Buğday Germ Aglütinin (WGA) Concanavalin-A (Con-A), Soya Fasulyesi Agglutinin (SBA) kullanılarak endometriyal bez salgılarının karbonhidrat içeriğinin tespit edilmesi amaçlanmıştır. Gereç Yöntem: Otuz iki Wistar Albino rat, her birinde 8 adet rat bulunan 4 gruba ayrıldı; diyabet ve gebelik pozitif, diyabet pozitif, gebelik pozitif, kontrol grubu. Numuneler gebeliğin 5. ve 7. günlerinde ve gebelik olmayan gruplarda da vajinal olarak stimule edilmiş sıçanlardan stimülasyonu izleyen 5. ve 7. günlerde toplandı. Bulgular: Her iki diyabetli grupta pozitif PNA boyaması meydana geldi ve diğer iki grupta boyanma olmadı. Diyabet, gebeliğin ilk günlerinde SBA boyamasında belirgin değişikliklere neden oldu, ancak uzun süre devam etmedi. WGA ile ilgili olarak, diyabet gebelikle birleştiğinde, endometriyal bezlerin salgılanmasında değişiklikler meydana geldiği görüldü. Con-A lektini ile yapılan boyamalar hem gebeliğin hem de diyabetin endometriyal bez salgılarının α-mannoz zinciri, α-zincir glikoz ve N-asetil glukozamin α-zincir bileşenlerinin miktarlarını etkilediğini göstermiştir. Sonuç: Sonuç olarak bu çalışma hem diyabetin hem de gebeliğin endometriyal bezlerden salgıların karbonhidrat içeriğini etkilediğini göstermektedir.

Does Diabetes Affect the Carbohydrate Secretions of the Endometrial Glands of Rats in Early Pregnancy?

nourishment for the embryo. Diabetes is known to alter the secretions of certain cells. This study aimed to identify thecarbohydrate content of endometrial gland secretions using Peanut Aglutinin (PNA), Wheat Germ Agglutinin (WGA)Concanavalin-A (Con-A), Soy Bean Agglutinin (SBA).Materials and Methods: Thirty-two Wistar Albino rats were divided into 4 groups of 8; diabetes and pregnancy positive,diabetes positive, pregnancy positive, control group. Samples were collected on the 5th and 7th day of pregnancy, and in thevaginally stimulated non-pregnant rats.Results: Positive PNA staining occurred in both groups with diabetes and was negative in the other two groups. Diabetesproduced marked alterations in SBA staining in the early days of pregnancy, but did not persist for long. With regard toWGA, when diabetes combined with pregnancy, changes in the secretion of the endometrial glands occur. Con-A stainingshowed that both pregnancy and diabetes affect the amounts of the α-mannose chain, α-chain glucose, and N-acetylglucosamine α-chain components of endometrial gland secretions.Conclusion: In conclusion this study shows that both diabetes and pregnancy affect the carbohydrate content of thesecretions from the endometrial glands.

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Akif F., Gabius H. J., & Danguy A. (1995). Estrus cyclerelated alterations in the expression of glycoconjugates and lectins in the Mouse endometrium shown histochemically. Tissue & Cell, 27(2), 197-206.
Burton G. J., Jauniaux E., & Charnock-Jones D. S. (2007). Human early placental development: potential roles of the endometrial glands. Placenta 28(Suppl. A, Troph Res (21), 64-69.
Bychkov V., & Toto P. D. (1987). Histochemical study of lectin binding to gestational epithelium. Int J Gynecol Pathol, 6, 66-72.
Bychkov V., & Toto P. D. (1986). Lectin binding to normal human endometrium. Gynecol Obstet Invest, 22, 29-33.
Cornejo-Garrido J., Becerril-Chávez F., Carlín-Vargas G., Ordoñez-Rodríguez J. M., Abrajan-González Mdel C., de la Cruz-Ramírez R., & OrdazPichardo C. (2014). Antihyperglycaemic effect of laser acupuncture treatment at BL20 in diabetic rats, Acupunct Med, 32(6), 486-494.
Dey S. K., Lim H., Das Sanjoy K., Reese J., Paria B. C., Daikoku T., & Wang H., (2004). Molecular cues to implantation, Endocrine Reviews, 25(3), 341– 373.
Gutaj P., Zawiejska A., Wender-Ożegowska E., & Brązert J. (2013). Maternal factors predictive of first trimester pregnancy loss in women with pregestational diabetes. Pol Arch Med Wewn. 123(1-2), 21-28.
Hempstock J., Cindrova-Davies T., & Jauniaux E.(2004). Endometrial glands as a source of nutrients, growth factors and cytokines during the first trimester of human pregnancy: a morphological and immunohistochemical study. Reprod Biol Endocrinol, 2, 58-63.
Isola M., Cossu M., & Diana M. (2012). Diabetes reduces statherin in human parotid: Immunogold study and comparison with submandibular gland. Oral Diseases,18(4), 360-364.
Jones C. J. P., Aplin J. D., Burton G. J. (2010) First Trimester Histiotrophe Shows Altered Sialylation compared with secretory phase glycoconjugates in human endometrium, Placenta, 315(76e), 580.
Jones C. J. P., Fazleabas A. T., & Mcginlay P. B. (1998) Cyclic modulation of epithelial glycosylation in human and baboon (Papio anubis) endometrium demonstrated by the binding of the agglutinin from Dolichos biflorus (DBA). Biol Reprod; 58, 20-27.
Kupryjancyzk J. (1989). Cycle and function related changes in lectin binding to human endometrium: a histochemical study with pronase treatment. Arch Gynecol Obstet, 246, 211-222.
Lee M.C., & Damjanov I. (1985). Pregnancy-related changes in the human endometrium revealed by lectin histochemistry. Histochemistry, 82, 275- 280.
Leitner M., Aurich J. E., & Galabova G. (2003). Lectin binding patterns in normal canine endometrium and in bitches with pyometra and cystic endometrial hyperplasia. Histol. Histopathol, 18, 787-795.
Lilliu M. A., Solinas P., & Cossu M. (2015). Diabetes causes morphological changes in human submandibular gland: a morphometric study. J Oral Patho. Med., 44(4), 291-295.
Lohmiller J. L., & Swing S. P. (2006). Reproduction and breeding in the laboratory rat, p.148-153 Academic Press, London (2006).
Lutsky A. & Sogomonian E. (2012). Structural, functional, and lectin histochemical characteristics of rat ovaries and endometrium in experimental hyper and hypothyroidism. Folia Histochemica et Cytobiologica, 10, 375-382.
Monsefi M., Hosseini E., & Alaee S. (2013). Lectin histochemical study of rat reproductive tissues treated with ether fraction of Anethum graveolens L. Extracts. Anatomical Sciences, 10, 41-49.
Munson L., Kao J. J., & Schlafer D. H. (1989). Characterization of glycoconjugates in the bovine endometrium and chorion by lectin histochemistry. J. Reprod. Fert, 87, 509-517.
Öztabak K. Ö. (2005). Lektinler ve viscum album agglutinin, Erciyes Üniv. Vet. Fak. Derg.; 2(1), 55-59.
Peel S. & Bulmer J. N. (1996). Lectin histochemistry of pregnant rat uterine tissues, J.Anat, 188, 197-205.
Stevens M. J., Li F., Drel V. R., Abatan O. I., Kim H., Burnett D., Larkin D., & Obrosova I. G. (2007). Nicotinamide reverses neurological and neurovascular deficits in streptozotocin diabetic rats. Journal of Pharmacology and Experimental Therapeutics; 320(1), 458-464.
Stewart J., Bebington C. R., & Mukhtar D. D. Y. (2000). Lectin binding characteristics of mouse placental cells. J. Anat., 196, 371-378.
Walter I. & Bavdek S. (1997). Lectin binding patterns of porcine oviduct mucosa and endometrium during the oestrus cycle. J.Anat, 190, 299-307.