Doğu Anadolu Kırmızısı Melez Sığırlarda Prolaktin Geni Polimorfizmi1

Bu çalışmada, yerli Doğu Anadolu Kırmızısı melezi sığırlarında Prolaktin genine ait polimorfik bölgeler PCR-RFLP yöntemiyle incelenmiş ve bölgelere ait gen ve genotip frekansları belirlenmiştir. Analizde 5'UTR bölgesi için HaeIII, 4.ekzon bölgelesi için RsaI restriksiyon enzimleri kullanılmıştır. PCR-RFLP analizi sonucunda PRL geni 5'UTR bölgesinde populasyon monomorfik bulunmuştur. PRL geni 4.ekzon bölgesinde AA, AB ve BB genotip frekansları melez populasyonda sırasıyla 0.49, 0.44. ve 0.07 olarak tespit edilmiştir. Prolaktin geni 4. ekzon RsaI polimorfizm bölgesinde populasyonun Hardy-Weinberg dengesinde (p>0.05) olduğu görülmüştür.

Polymorphism of Prolactin Gene in the East Anatolian Red Crosbreds

In this study, in the native East Anatolian Red Crosbreds, different polymorphic site of Prolactin gene was examined by PCRRFLP method and the gene and genotype frequencies were determined. In the analysis of 5'UTR, we used HaeIII while RsaI restriction enzyme for exon 4. PCR-RFLP analysis was resulted with monomorphic genotype for 5'UTR in the population. AA, AB and BB genotype frequencies in exon 4 were 0.49, 0.44 and 0.07 for crosbreeds EAR. In RsaI polymorphic site of the prolactin exon 4 the population was in the H-W equilibrium (p>0.05).

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