Deneysel Diyabetli Ratlarda Timokinon Uygulanmasının Doku Total Oksidan ve Antioksidan Düzeyine Etkisi

Bu çalışmada, streptozotosin (STZ) ile diyabet oluşturulmuş ratlarda timokinon uygulanmasının karaciğer ve böbrek dokularında total antioksidan kapasite (TAS), total oksidan kapasite (TOS) ve oksidatif stres indeksine (OSİ) etkileri araştırıldı. Materyal olarak 24 adet 200-250 g ağırlığında Wistar-albino erkek rat kullanıldı. Ratlar, her biri altı rattan oluşan; kontrol (K), timokinon (TQ), diyabet (D) ve diyabet+timokinon (DT) grupları olmak üzere dört gruba ayrıldı. Diyabet oluşturmak için D ve DT grubundaki ratlara 45 mg/kg tek doz STZ i.p. yolla uygulandı. Timokinon, DT ve TQ grubundaki ratlara 30 mg/kg/21 gün olarak gavaj yoluyla verildi. Çalışma sonunda karaciğer ve böbrek dokularında TAS ve TOS değerleri ölçüldü. OSİ, TAS ve TOS düzeylerine göre hesaplandı. Karaciğer dokusunda TAS, D grubunda en düşük olarak saptandı (P≤0.05). DT grubu ile K ve TQ grupları arasında fark olmadığı görüldü. Karaciğer TOS bakımından gruplar arasında bir fark saptanmadı. Karaciğerde OSİ, K’e göre bütün gruplarda arttığı (P≤0.05), en yüksek değerin D grubunda olduğu, TQ uygulanmasından sonra kısmen azalarak K’e yaklaştığı tespit edildi. Böbrek dokusunda TAS ve TOS düzeyleri TQ grubunda en yüksek, D grubunda en düşük bulunurken (P≤0.05), diğer gruplar arasında bir fark bulunmadı. Böbrek OSİ’de ise gruplar arasında önemli bir fark saptanmadı. Sonuç olarak, deneysel diyabetli ratların karaciğer ve böbrek dokularındaki TOS, TAS durumunun TQ uygulanmasından etkilendiği, karaciğerde STZ uygulanan gruplarda TQ uygulanmasından sonra OSİ değerinin K grubuna yaklaştığı saptandı.

The Effect of Thymoquinone Treatment on Total oxidant and Total Antioxidant Level in Experimental Diabetic Rats

In this study, the effects of thymoquinone implementation to total antioxidant status (TAS), total oxidant status (TOS) and oxidative stress index (OSI) were investigated in liver and kidney tissues of diabetic rats induced with STZ. The 24 male Wistar-Albino rats, weighing between 200-250 g were used as material. Rats were divided into four groups, each consisting of six rats; control (C), thymoquinone (TQ), diabetes (D) and diabetes+ thymoquinone (DT) groups. To induce diabetes, a single dose of 45mg/kg streptozotocin (STZ) were administered intraperitoneally (ip) to the rats in groups D and DT. TQ was administered to the rats in DT and TQ groups as 30 mg/kg/21 days by oral gavage. The values of TAS and TOS were measured in liver and kidney tissues at the end of the study. OSI was calculated according to TAS and TOS levels. TAS value in liver tissue was found to be the lowest in the diabetes group (P≤0.05). It was noticed that there was no difference between K and TQ groups with DT group. No difference was detected among groups in terms of liver TOS. It was determined that the OSI in the liver increased in all groups compared to the control (P≤0.05), the highest value was determined in group D, it approached to the control by partially decrease after the application of TQ. While TAS and TOS levels in kidney tissue were found to be the highest in the TQ group and the lowest in the diabetic group (P≤0.05), there was no difference between the other groups. There was no significant difference among the groups in the kidney OSI. As a result, it was determined that TOS, TAS values in liver and kidney tissues of experimental diabetic rats were affected from the TQ application and the OSI value in the liver of the STZ treated groups approached that of the control group, after TQ application.

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