Mehmet GÜLTEKİN, Hasan ERDOĞAN, Kerem URAL, Yasin PARLATIR, Serdar PAŞA, Canberk BALIKÇI, Songül TOPLU

Ehrlichiosis’li Köpeklerde D-dimer/Fibrinojen Oranı

Tromboemboliler kanama bozuklukları ile seyreden hastalıklarda mortalite düzeylerini etkileyen önemli sorunlardanbiridir. Ehrlichiosis gibi kanama bozukluğuna neden olabilen hastalıklarda derin ven trombozları ve tromboembolilerinşekillendiği bilinmekte ancak tanının konulabilmesi için ileri diyagnostik tekniklere ihtiyaç duyulmaktadır. Bu çalışmada,Ehrlichiosis ile monoenfeksiyonlu köpeklerde D-dimer testi ile D-dimer/Fibrinojen oranlarının belirlenmesi amaçlandı. Buamaçla yüksek ateş lenfadenopati ve iştahsızlık gibi klinik bulgular gösteren ve hasta başı hızlı ELİSA testi Snap 4dx sonuçlarınagöre Ehrlichiosis ile monoenfeksiyonlu köpekler (n=10) ile klinik ve laboratuvar değerlendirmeleri sonucunda sağlıklı olduğubelirlenen (n=10) köpekler çalışmaya alındı. Çalışma gruplarında bulunan köpeklerden EDTA ve sitratlı kan örnekleriV.cephalica antebrachi üzerinden toplamda 5 ml olacak şekilde alınarak D-dimer ve Fibrinojen seviyeleri ticari test kitleriyardımı ile D-dimer/Fibrinojen oranı ise hesaplanarak elde edildi. Ehrlichiosis ile monoenfeksiyonlu köpeklerin D-dimer(3059.01074.4 ng/ml) ve Fibrinojen (371.062.0 mg/dl) seviyelerinin ve D-dimer/Fibrinojen oranlarının (12.25.9) sağlıklıköpeklere göre istatistiksel olarak (P=0.011) yüksek bulundu. Sonuç olarak Ehrlichiosis ile monoenfeksiyonlu köpeklerde Ddimer/Fibrinojen oranının ileri diyagnostik teknikler ile birleştirilerek Veteriner sahada tromboembolilerin tanısal anlamdayaklaşımına ışık tutabilecek biyobelirteçler arasına girebileceği düşünüldü.

D-dimer / Fibrinogen Ratio in Dogs with Ehrlichiosis

Thromboembolism is one of the important problems affecting mortality levels in diseases with bleeding disorders. It is known that deep venous thrombosis and thromboembolism are formed in diseases such as Ehrlichiosis which may cause hemorrhagic disorder but advanced diagnostic techniques are needed for a precise diagnosis. In this study, the aim was to determine D-dimer / fibrinogen ratios by D-dimer test in monoinfected dogs with Ehrlichiosis. For this purpose, dogs presenting high fever, lymphadenopathy and anorexia to those of mono-infected with Ehrlichiosis as detected by point of care rapid ELISA test Snap 4dx along within healthy ones (n=10) as determined within clinical and laboratory evaluation. Enrolled dogs in the present study were subjected to withdrawal of a total of 5 ml EDTA and citrated blood samples from V.cephalica antebrachi in an attempt to calculate D-dimer / Fibrinogen ratio by use of D-dimer and Fibrinogen levels as detected by commercial test kits. D-dimer (3059.01074.4 ng/ml) and Fibrinogen (371.062.0 mg/dl) levels and D-dimer / Fibrinogen ratios (12.25.9) of monoinfected dogs with Ehrlichiosis were found significantly elevated (P=0.011) in contrast to healthy dogs. In conclusion, it was suggested that the ratio of D-dimer/Fibrinogen in dogs with Ehrlichiosis monoinfection even combined with advanced diagnostic techniques, might become a biomarker that can shed light on the diagnostic significance of veterinary field thromboembolisms.

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