YAŞAR NAKİPOĞLU, SEYDA İĞNAK, Bülent GÜRLER, Zayre ERTURAN, Derya AYDIN

Metisiline duyarlı ve dirençli Staphylococcus aureus suşlarının antiseptik ve dezenfektan maddelere duyarlılıklarının karşılaştırılması

Çalışmamızda üçü antiseptik [% 70 izopropil alkol (İPA), % 10 povidon-iyot, % 10 povidon-iyot/etanol], biri çamaşır suyu olmak üzere toplam dört preparata karşı metisiline duyarlı (MS) 50 ve metisiline dirençli (MR) 50 S.aureus (SA) suşunun duyarlılıkları karşılaştırılmıştır. Sonuç olarak MSSA ve MRSA suşları arasında % 10 povidon-iyot ve % 10 povidon-iyot/etanole direnç açısından anlamlı fark bulunmamış (p>0.05) olmakla birlikte, MRSA suşlarında % 70 İPA’ya (p

Anahtar Kelimeler:

Mikrobiyal duyarlılık testleri, Anti-enfektif ajanlar, lokal, Dezenfektanlar, Povidon-iyot, Metsilin-dirençli Staphylococcus aureus

Comparison of antiseptic and disinfectant susceptibility in methicillin-susceptible and resistant Staphylococcus aureus strains

In this study, the susceptibility of 50 methicillin-susceptible (MS) and 50 methicillin-resistant (MR) Staphylococcus aureus (SA) strains for three antiseptics [70 % isopropyl alcohol (IPA), 10 % povidon-iodine, 10 % povidon-iodine/ethanol] and one disinfectant (house bleach) was investigated. It was found that there was no siginificant differences between the resistance of MSSA and MRSA strains to 10 % povidon-iodine and 10 % povidon-iodine/ethanol (p>0.05). Significant higher resistance in MRSA strains to 70 % IPA (p<0.01) and to house bleach (p<0.001) were detected comparing with MSSA strains.

Keywords:

Microbial Sensitivity Tests, Anti-Infective Agents, Local, Disinfectants, Povidone-Iodine, Methicillin-Resistant Staphylococcus aureus,

PDF

___

1. Behr H, Reverdy ME, Mabilat C, Freney J, Fleurette J: Relation entre le niveau des concentrations minimales inhibitrices de cinqantiseptiques et la presence du gene qacA chez Staphylococcus aureus, Pathol Biol (Paris) 1994;42(5):438-44.
2. Dussau JY, Chapalain JC, Rouby Y, Reverdy ME, Bartoli M: Evaluation par une micromethode de l'activite bactericide de cinq disinfectants sur 108 souches hospitalieres, Pathol Biol (Paris) 1993;41(4):349-57.
3. Kampf G, Jarosch R, Ruden H: Wirksamkeit alkoholischer handedesinfektionsmittel gegenuber mithicillin-resistenten Staphylococus aureus (MRSA), Chirurg 1997;68(3):264-8.
4. McDonnel G, Russell AD: Antiseptics and disinfectants: activity, action, and resistance, Clin Microbiol Rev 1999;12(l):147-79.
5. Mycock G: Methidllin/antiseptic-resistant Staphylococcus aureus, Lancet 1985;2(8461):1081-2.
6. National Committee for Clinical Laboratory Standards (NCCLS): Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically, NCCLS Approved Standard M7-A6,Wayne, Pa (2003).
7. Prince HN, Nonemaker WS, Norgard RC, Prince DL: Drug resistance studies with topical antiseptics, J Pharm Sci 1978;67(ll):1629-3l.
8. Reverdy ME, Bes C, Nervi C, Martra A, Fleurette J: Activity of four antiseptics (acriflavine, benzalkonium chloride, chlorhexidine digluconate and hexamidine di-isethionate) and of ethidium bromide on 392 strains representing 26 Staphylococcus species, Med Microbiol Lett 1992;1:56.
9. Sivaji Y, Mandal A, Agarwal DS: Disinfectant induced changes in the antibiotic sensitivity and phage typing pattern in Staphylococcus aureus, J Hosp Infect 1986;7(3):236-43,