Altan AKSOY, J. Sedef GÖÇMEN, BİRGÜL KAÇMAZ, Selda CANVER

İnsan ve sığırlardan izole edilen fekal Escherichia coli suşlarında antibiyotik direnci ve genişlemiş spektrumlu beta-laktamaz üretimi

Son bir ay içinde antibiyotik kullanmamış insanların dışkılarından izole edilen herbiri farklı bireye ait 140 Escherichia coli susu ile sığırların rektum mukozasından izole edilen 114 E.coli susunun 10 antibiyotiğe duyarlılığı disk difüzyon yöntemi ile, bu suşlarda genişlemiş spektrumlu beta-laktamaz (GSBL) üretimi fenotipik doğrulama testi ile belirlenmiştir. İnsan suşlannda ampisilin, ampisilin-sulbaktam, amoksisilin-klavulanik asit, piperasilin-tazobaktam, sefotaksim, siprofloksasin, trimetoprim-sulfametoksazol direnci sığır suşlarındakinden, sığır suşlannda amikasin direnci insan suşlanndakinden anlamlı derecede daha yüksek bulunmuştur. Seftazidim ve gentamisin direnci insan suşlannda daha yüksek bulunmuşsa da fark anlamlı değildir. İki grup susta da en yüksek direnç ampisilin için bulunmuştur. İnsan suşlannda direnç cinsiyete göre fark göstermemiş, ancak 13 yaş ve altındaki çocuklara ait suşlarda büyüklerden izole edilenlere göre ampisilin, ampisilin-sulbaktam, seftazidim, gentamisin ve trimetoprim-sulfametoksazol direnci anlamlı derecede yüksek bulunmuştur. İnsan suşlannda GSBL üretimi % 3 olarak saptanmış, sığır suşlannda GSBL üretimine rastlanmamıştır.

Anahtar Kelimeler:

Rektum, Sığır, Escherichia coli, Dışkı, İnsan, Mikrobiyal duyarlılık testleri, İlaç direnci, bakteriyel

Antibiotic resistance and extended spectrum beta-lactamase production in fecal Escherichia coli strains isolated from human and cattle

A hundred forty E.coli strains isolated from stools of humans who did not use antibiotics in the last one month and 114 E.coli strains isolated from cattle's rectal mucosa were enrolled in this study. Each strain was from different individuals. Susceptibility of these strains to ten antibiotics were determined by disk diffusion test and extended spectrum beta-lactamase (ESBL) production by phenotypic confirmation test. The resistance to ampicillin, ampicillin-sulbactam, amoxicilin-clavulanic asid, piperacillin-tazobactam, sefotaxime, ciprofloxacin and trimethoprim-sulfamethoxazole in human E. coli strains were found significantly higher than the resistance of cattle E. coli strains and the resistance to amikacin in cattle E. coli strains was found significantly higher than the resistance of human E. coli strains. Although the resistance to ceftazidime and gentamicin in human E.coli strains were found higher, the differences was not statistically significant. The highest resistance was for ampicillin in both cattle and human strains. The differences in antibiotic resistance of human strains were insignificant in two genders. But the antibiotic resistance were significantly higher in the strains from children under 13 age than the strains from adults for ampicillin, ampicillin-sulbactam, trimetoprim-sulfamethoxazole, gentamisin and ceftazidime. While ESBL production from human strains was 3 %, there was no production in cattle strains.

Keywords:

Rectum, Cattle, Escherichia coli, Feces, Humans, Microbial Sensitivity Tests, Drug Resistance, Bacterial,

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1. Berends BR, van den Bogaard AE, van Knapen F, Snijders JM: Human health hazards associated with the administration of antimicrobials to slaughter animals. Part II.An assessment of the risks of resistant bacteria in pigs and pork, Vet Q 2001;23:10-21.
2. Bonten M, Stobberingh E, Philips J, Houben A: High prevalence of antibiotic resistant Escherichia coli in faecal samples of students in the south-east of The Netherlands, JAntimicrob Chemother 1990;26:585-92.
3. Bonten M, Stobberingh E, Philips J, Houben A: Antibiotic resistance of Escherichia coli in fecal samples of healthy people in two different areas in an industrialized country, Infection 1992;20:258-62.
4. Bopp CA, Brenner FW,Wells JG, Strockbine NA: Escherichia, Shigella, Salmonella, “Murray PR, Baron EJ, Pfaller MA, Tenover FC, Yolken RH (eds): Manual of Clinical Microbiology, 7. baskı” kitabında s.459, AMS Press, Washington (1999).
5. Degener JE, SmitAC,MichelMF,Valkenburg HA, Muller L: Resistance of Escherichia coli in faeces and the use of antimicrobial agents in the treatment of hospital patients, J Hyg (Lond) 1983;91:491-8.
6. Degener JE, Smit AC, Michel MF, Valkenburg HA, Muller L: Faecal carriage of aerobicGram-negative bacilli and drug resistance of Escherichia coli in different age-groups in Dutch urban communities, JMedMicrobiol 1983;16:139-45.
7. Demirdağ K, Kizirgil A, Özden M, Kalkan A, Felek S, Toraman ZA: Hastane ve toplum kökenli Klebsiella pneumoniae ve Escherichia coli suşlarında genişletilmiş spektrumlu beta-laktamaz sıklığının araştırılması, ANKEM Derg 2001;15:748-52.
8. Gürdoğan K, Arslan H: Hastane kökenli ve hastane dışı E. coli’lerde çift disk sinerji yöntemiyle genişlemiş spektrumlu beta-laktamaz araştırılması ve izolatların çeşitli antibiyotiklere duyarlılık durumu, Flora 1999;4:177-80.
9. Lester SC, del Pilar Pla M, Wang F, Perez Schael I, Jiang H, O'Brien TF: The carriage of Escherichia coli resistant to antimicrobial agents by healthy children in Boston, in Caracas, Venezuela, and in Qin Pu, China, N Engl J Med 1990;323:285-9.
10. Levy SB,Marshall B,Schluederberg S, Rowse D,Davis J:High frequency of antimicrobial resistance inhuman fecal flora,AntimicrobAgentChemother 1988;32:1801-6.
11. Linton KB, Lee PA, Richmond MH, GillespieWA, Rowland AJ, Baker VN: Antibiotic resistance and transmissible R-factors in the intestinal coliform flora of healthy adults and children in an urban and a rural community, J Hyg (Lond) 1972;70:99-104.
12. Macias AE, Herrera LE, Munoz JM, Medina H: Antibiotic-resistant fecal Escherichia coli in healthy children. Induced by the use of antibiotics?, Rev Invest Clin 2002;54:108-12.
13. National Committee for Clinical Laboratory Standards: Performance Standards forAntimicrobial Susceptibility Testing;Twelfth Informational Supplement M2-A7 (M100-S12), Wayne, PA (2002).
14. Sayah RS, Kaneene JB, Johnson Y, Miller R: Patterns of antimicrobial resistance observed in Escherichia coli isolates obtained from domesticand wild-animal fecal samples, human septage, and surface water, Appl Environ Microbiol 2005;71:1394-404.
15. Stobberingh EE, van den Bogaard AE: Spread of antibiotic resistance from food animals to man, Acta Vet Scand 2000;93(Suppl):47-50.
16. Tauxe RV, Cavanagh TR, Cohen ML: Interspecies gene transfer in vivo producing an outbreak of multiply resistant shigellosis, J Infect Dis 1989;160:1067-70.
17. Tünger Ö, Sürücüoğlu S, Özbakkaloğlu B, Gazi H: Toplumsal kökenli ve nozokomiyal idrar yolu infeksiyonlarından izole edilen Escherichia coli suşlarında genişlemiş spektrumlu beta-laktamaz varlığının araştırılması ve çeşitli antibiyotiklere duyarlılıkları, Mikrobiyol Bült 2001;35:351-7.
18. Valverde A, Coque TM, Sanchez-Moreno MP, Rollan A, Baquero F, Canton R: Dramatic increase in prevalence of fecal carriage of extendedspectrumbeta- lactamase-producing Enterobacteriaceae during nonoutbreak situations in Spain, J Clin Microbiol 2004;42:4769-75.