Md Amjad BEG, Mustafa SEVİNDİK, Shahid HAİDER, Preeti SONİ, Priya BHATİA, Shahzul HASAN, Richa YADAV, Fareeda ATHAR

RV1463 OLASI KORUNMUŞ ATP BAĞLAYICI PROTEİNİN HESAPLAMALI YAKLAŞIMLA MOLEKÜLER TEMEL BÜTÜNLEŞTİRİCİ ANALİZİ

Amaç: Tüberküloz, patojeni olan Mycobacterium tuberculosis H37Rv'nin (M. tuberculosis) şiddetle değişen dinamiği nedeniyle yıllardan beri küresel bir salgın olmuştur. Bu patojenin durumu daha da kötüleşmektedir ve üstesinden gelmek zorlaşmaktadır. Bu yazıda, metal iyonunun plazma membranları boyunca taşınmasına yardımcı olan ve sonuçta bir elektrokimyasal gradyan oluşturan Mycobacterium tuberculosis H37Rv'nin (M. tuberculosis) ATP bağlayıcı proteini için hesaplama yaklaşımlarını kullandı. Rv1463, ATP bağlama motifi (WalkerA) (GXXXXGKS / T) ve (Walkera) (DEXXXXXD) içeren varsayımsal bir protein ve bu motiflerin ATP bağlama ve hidrolizleme aktivitelerindeki önemi üzerinde duruldu. ATP bağlanma özelliğini, transkripsiyonel düzenleyici protein ile etkileşime girerek ve etkileşimli bileşikler magnezyum (Mg) ve Adenosin di fosfat (ADP) ile gösterir.

MOLECULAR BASIS AND INTEGRATIVE ANALYSIS OF Rv1463 AS PROBABLE CONSERVED ATP-BINDING PROTEIN BY COMPUTATIONAL APPROACH

Objective: Tuberculosis as a global epidemic since years due to vigorously changing dynamics of its causal pathogen, Mycobacterium tuberculosis H37Rv (M. tuberculosis). This pathogen has worsened the situation therefore making it so challenging and hard to overcome. In this manuscript, we have used the computational approaches for ATP-binding protein of Mycobacterium tuberculosis H37Rv (M. tuberculosis) that helps in transportation of metal ion across the plasma membranes and resultant generating an electrochemical gradient. Rv1463 a hypothetical protein possessing ATP binding motif (WalkerA) (GXXXXGKS/T), and (Walker B) (DEXXXXXD) and significance of these motifs in ATP binding and hydrolyzing activities. It shows the ATP-binding property by interacting with transcriptional regulatory protein and showing the interacted compounds as magnesium (Mg) and Adenosine di phosphate (ADP).

Keywords:

Molecular docking, Mycobacterium tuberculosis, Rv1463, tuberculosis,

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