HPTLC İLE CALOTROPIS GIGANTEA VE CALOTROPIS PROCERA YAPRAĞINDAKİ β-SİTOSTEROLÜN TANIMLANMASI VE MİKTAR TAYİNİ
Amaç: Asclepidiaceae familyasına ait Calotropis procerave Calotropis gigantean yaprağında bulunan biyomarker β-sitosterol'ün ayırt edici kanıtı ve ölçümü, yüksek performanslı ince tabaka kromatografik (HPTLC) karakterizasyon yöntemi kullanılarak gerçekleştirildi. Bu çalışmada, kantitatif değerlendirme için dış standart yöntemi kullanılmıştır.
IDENTIFICATION AND QUANTIFICATION OF β- SITOSTEROL IN LEAF OF CALOTROPIS GIGANTEA AND CALOTROPIS PROCERA BY HPTLC
Objective: The distinguishing proof and measurement of biomarker β-sitosterol present in a leaf of Calotropis procera and Calotropis gigantea belonging to family Asclepidiaceae was carried by utilizing high performance thin-layer chromatographic (HPTLC) characterization method. In the present research work the Exterinsic Standard Method is used for quantitative assessment.
___
- 1. Aparna, S., Aruna, S. (2015). HPTLC Method for Identification and Quantification of βsitosterol in the Roots of Achyranthesaspera Linn. International Journal of Pharmaceutical Science, 5(4), 1117-1122. https//doi.org/10.7598/cst2015.1137
- 2. Midmore, D.J. (2017). Principles of tropical horticulture. The Future of Tropical Horticulture, (pp. 353-418). Oxfordshire, UK. https://doi.org/10.1079/9781780645414.035
- 3. Khairnar, A.K., Bhamare, S.R., Bhamare, H.P. (2012). Calotropis procera: An ethnopharmacological update. Advance Research in Pharmaceuticals and Biologicals, 2(2), 142-156. https://doi.org/10.22159/ajpcr.2017.v10i11.21215
- 4. Rahman, M., Wilcock, C. (1991). A taxonomic revision of Calotropis (Asclepiadaceae). Nordic Journal of Botany, 11(3), 301-308. http://dx.doi.org/10.1111/j.1756-1051.1991.tb01408.x
- 5. Chatterjee, A., Chandra, P.S. (1995). The Treatise of Indian Medicinal Plants: Volume: IV. Nat Inst of Sc Communication, New Delhi, India.
- 6. Shripad, M.B., Prashant, G.J., Gilhotra, R. (2018). Pharmacology of Natural Products: A recent approach on Calotropis gigantea and Calotropis procera. ArsPharmaceutica, 59(1), 37-44. http://dx.doi.org/10.4321/S2340-98942018000100004
- 7. Palejkar, C.J., Palejkar, J.H., Patel, M.A., Patel, A.J. (2012). A comprehensive review on plant Calotropis gigantea. International Journal of Institutional Pharmacy and Life Sciences, 2(2), 463-470.
- 8. Abhishek, D., Mohit, C., Ashish, G., Ameeta, A. (2010). Medicinal utility of Calotropis procera (Ait.) R. Br. as used by natives of village Sanwer of Indore District, Madhya Pradesh. International Journal of Pharmacy & Life Sciences, 1(3), 188-190. http://dx.doi.org/10.18203/2319-2003.ijbcp20161484
- 9. Al-Snafi, A.E. (2015). The constituents and pharmacological properties of Calotropis proceraan overview. International Journal of Pharmacy Review and Research, 5(3), 259-275.
- 10. Kitagawa, I., Zhang, R.S., Park, J.D., Baek, N.I, Takeda, Y., Yoshikawa, N., Shibuya, H. (1992). Indonesian plants, I. chemical structures of calotroposides A and B two new oxypreganeoligoglycosides from the root of Calotropis gigantea (Asclepiadaceae). Chemical and Pharmaceutical Bulletin, 140, 2007-2013. https://doi.org/10.1248/cpb.31.689
- 11. Pari, K., Rao, P.J., Devakumar, C., Rstogi, J.N. (1998). A novel insect antifeedantnonprotein amino acid from Calotropicsgigantean. Journal of Natural Product, 61, 102-104. doi: 10.1021/np970255z.
- 12. Sen, S., Sahu, N.P., Mahato, S.B. (1992). Flavonol glycosides from Calotropicsgigantean. Phytochemistry, 31, 2919-2921. doi: 10.1016/0031-9422(92)83668-o.
- 13. Khandelwal, K. (2008). Practical Pharmacognosy, Techniques and Experiments, Nirali Prakashan, India, p.320.
- 14. Mukherjee, P.K. (2002). Quality Control of Herbal Drugs- An Approach to Evaluation of Botanicals, Business Horizons, p.800.
- 15. Shah, S.A., Ravishankara, M.N., Nirmal, A., Shishoo, C.J., Rathod, I.S., Suhagia, B.N. (2000). Estimation of individual sennosides in plant materials and marketed formulations by HPTLC method. Journal of Pharmacy and Pharmacology, 52, 445-449. https://doi.org/10.1211/0022357001774066
- 16. Stahl, E. (1969). Thin Layer Chromatography-A Laboratory Handbook, Springer-Verlag Berlin Heidelberg, p.1041. https://www.springer.com/gp/book/9783642884900
- 17. Shaikh, B.T., Hatcher, J. (2005). Complementary and alternative medicine in Pakistan: prospects and limitations. Evidence Based Complementary and Alternative Medicine, 2(2), 139- 142. https://doi.org/10.1093/ecam/neh088
- 18. Kunle, O.F., Egharevba, H.O., Ahmedu, P.O. (2012). Standardization of herbal medicines-A review. International Journal of Biodiversity and Conservation, 4(3), 101-112. https://doi.org/10.5897/IJBC11.163
- 19. Chawla, R., Thakur, P., Chowdhry, A., Jaiswal, S., Sharma, A., Goel, R., Sharma, J., Priyadarshi, S.S., Kumar, V., Sharma, R.K., Arora, R. (2013). Evidence based herbal drug standardization approach in coping with challenges of holistic management of diabetes: a dreadful lifestyle disorder of 21st century. Journal of Diabetes and Metabolic Disorders, 12(1), 35. https://doi.org/10.1186/2251-6581-12-35
- 20. Niyati, S.A., Sanjeev, R.A., Mamta, B.S., Dev, D.S. (2012). Development of pharmacognostical parameters and estimation of β-sitosterol using HPTLC in roots of Gmelina arborea Roxb. Pharmacognosy Journal, 30(4), 1-9