Dışsal sentetik inositol türevi (Allo-İnositol) uygulamasının Capsicum chinense bitkisinin tuz (NaCl) toleransı üzerine etkisi
Bu çalışmada tuz stresine maruz bırakılan Capsicum chinense (biber) bitkisinde, dışsal olarak yapraklardan uygulanan sentetik siklitol türevi allo-inositol’ün tuz tolerans mekanizmasında oynadığı rollerin fizyolojik ve biyokimyasal yöntemlerle araştırılması amaçlanmıştır. Bu kapsamda tuz stresine maruz bırakılan bitkiler (150 µM NaCl) gerek kontrol bitkileri gerekse siklitol uygulanmış stres gruplarıyla kıyaslanarak; yaprak su potansiyelleri, antioksidan enzim aktiviteleri, lipid peroksidasyonları (MDA), hidrojen peroksit (H2O2), prolin ve kalsiyum miktarları belirlenmiştir. Bulgularımıza göre stres gruplarında allo-inositol uygulaması yaprak su potansiyelini, prolin miktarını, kalsiyum içeriğini ve antioksidan enzim aktivitelerini arttırırken, MDA ve H2O2 içeriğini ise azaltmıştır. Bu sonuçlara göre; dışsal olarak uygulanan sentetik allo-inositol’ün biber bitkisinde tuz stresinin olumsuz etkilerini hafifletebildiği söylenebilir.
The effect of exogenous application of Synthetic inositol derivative (Allo-Inositol) on salt tolerance of Capsicum chinense
In this study, it is aimed to investigate the role of synthetic cyclitol derivative allo-inositol on salt tolerance mechanism in the Capsicum chinense (pepper) which is exposed to salt stress by physiological and biochemical methods. Plants thatexposed to salt stress (150 µM NaCl) compared to both control plants and cyclitol applied stress groups; leaf water potentials, antioxidant enzyme activities, lipid peroxidation (MDA), hydrogen peroxide (H2O2), proline and calcium levels were determined. According to our findings, application of allo-inositol in stressed groups increased leaf water potential, proline amount, calcium content, and antioxidant enzyme activities while decreasing MDA and H2O2 content. According to these results; it can be said that the synthetic allo-inositol which exogenously applied to pepper plant may alleviate the negative effects of salt stress.
___
- AbdElgawad, H., Zinta, G., Hegab, M.M., Pandey,
R., Asard, H., Abuelsoud, W., 2016. High salinity
induces different oxidative stress and antioxidant
responses in maize seedlings organs. Frontiers in
Plant Science, 7: 276.
- Aebi, H. 1974. Catalase. In: Bergmeyer, H.U. (Eds.)
Methods of enzymatic analysis, Verlag
Chemie/Academic Press Inc., NewYork, pp. 673-
680.
- Ahmad, P., 2010. Growth and antioxidant responses in
mustard (Brassica napus L.) plants subjected to
combined effect of gibberellic acid and salinity.
Archieves Agronomy and Soil Science, 56: 575-588.
- Alpaslan, M., Gunes, A., Taban, S., Erda, I., Tarakciogl
u, C., 1998. Variations in calcium, phosphorous,
iron, copper, zinc, and manganese contents of wheat
and rice varieties under salt stress, Turkish
Journal.of Agriculture.and Forestry, 22: 227–233.
- Ashraf, M., Iram, A., 2005. Drought stress induced
changes in some organic substances in nodules and
other plant parts of two potential legumes differing
in salt tolerance. Flora-Morphology, Distribution,
Functional Ecology of Plants, 200 (6): 535-546.
- Avcı, M.A., 2015. Tuzlu koşullarda yetiştirilen mısır
bitkisine diüre ve buğday bitkisine mannitol
uygulamasının bitki fizyolojisine ve beslenmesine
etkileri. Yüksek Lisans Tezi, Harran Üniversitesi
Fen Bilimleri Enstitüsü, Şanlıurfa.
- Baran, A., Doğan, M., 2014. Tuz stresi uygulanan
soyada (Glycine max L.) salisilik asidin fizyolojik
etkisi. Süleyman Demirel University Journal of
Natural and Applied Science, 18(1): 78-84.
- Bartoli, C.G., Simontacchi, M., Tambussi, E., Beltrano,
J., Montaldi, E., Puntarulo, S., 1999. Drought and
watering-dependent oxidative stres: effect on
antioxidant content in Triticum aestivum L. leaves.
Journal of Experimental Botany, 50: 375-383.
- Bates, L.S., Waldren, R.P., Teare, I.D., 1973. Rapid
determination of free proline for water-stress studies.
Plant and Soil, 39 (1): 205-207.
- Beyer, W.F., Fridowich, I., 1987. Assaying for
superokside dismutase activity: Some large
consequences of minor changes in conditions.
Analytical Biochemistry, 161: 559-566.
- Bieleski, R.L., 1994. Pinitol is a major carbohydrate in
leaves of some coastal plants indigenous to New
Zealand. New Zealand Journal of Botany, 32 (1):
73–78.
- Bonnet, M., Camares, O., Veisserie, P., 2000. Effects of
zinc and influence of Acremonium lolii on growth
parameters, chlorophyll a flurescence and
antioxidant enzyme activites of ryegrass. Journal
Experimental Botanany, 51: 945-953.
- Boyer , J.S.,1982. Plant productivity and environment.
Science, 218: 443-8.
- Bradford, M.M., 1976. A rapid and sensitice method for
the quantification of microgram quantities of
protein, utilizing the principle of protein-dye
binding. Analytical Biochemistry, 72: 248-254.
- Calberg, I., Mannervik, B., 1985. Glutathion Reductase:
In Methods in Enzymology. Academic Press, 113:
484-490.
- Cicerali, I.N., 2004. Effect of salt stress on antioxidant
defense systems of sensitive and resistant cultivars
of lentil (Lens culinaris M.). Doktora Tezi, Ortadoğu
Teknik Üniversitesi Fen Bilimleri Enstitüsü, Ankara.
Çelik, Ö., Atak, Ç., 2012. The effect of salt stress on
antioxidative enzymes and proline. Turkish. Journal
of Biology, 36: 339-356.
- Çevik, S., Akpınar, G., Yıldızlı, A., Kasap, M.,
Karaosmanoğlu, K., Ünyayar, S., 2019a.
Comparative physiological and leaf proteome
analysis between drought-tolerant chickpea Cicer
reticulatum and drought-sensitive chickpea C.
arietinum. Journal of Bioscience, 44(20): 1-13.
- Çevik, S., Güzel Değer, A., Yıldızlı, A., Gök, A.,
Ünyayar, S., 2019b. Proteomic and physiological
analyses of dl-cyclopentane-1,2,3-triol-treated barley
under drought stress. Plant Molecular Biology
Reporter, 37(3): 237-251.
- Çevik, S., Ünyayar, S., 2015. The effects of exogenous
application of ascorbate and glutathione on
antioxidant system in cultivated Cicer arietinum and
wild type C. reticulatum under drought stress. SDU
Journal of Natural and Applied Sciences, 19: 1-13.
- Çevik, S., Yıldızlı, A., Yandım, G., Göksu, H.,
Gultekin, M.S., Değer, A.G., Celik, A., Kuş, N.Ş.,
Ünyayar, S., 2014. Some synthetic cyclitol
derivatives alleviate the effect of water deficit in
cultivated and wild-type chickpea species. Journal of
Plant Physiology, 171 (10): 807-816.
- Çömlekçioğlu, N., Arıkan, S., 2017. Fizyolojik stres ve
eksojen poliaminlerin Isatis tinctoria L.
yapraklarındaki indigo miktarı ve fide gelişimi
üzerine etkisi. Mediterranean Agrıcultural Sciences,
30 (3): 261-267.
- Çulha, Ş., 2011. Tuz stresinin aspir (Carthamus
tinctorius L.) çeşitlerindeki bazı fizyolojik ve
biyokimyasal parametreler üzerine etkisinin
incelenmesi. Yüksek Lisans Tezi, Hacettepe
Üniversitesi Fen Bilimleri Enstitüsü, Ankara.
- Deveci, M., Tuğrul, B., 2017. Ispanakta tuz stresinin
yaprak fizyolojik özelliklerine etkisi. Akademik
Ziraat Dergisi, 6: 89-98.
- Eisa, S., Hussin, S., Geissler, N., Koyro H.W., 2012.
Effect of NaCl salinity on water relations,
photosynthesis and chemical composition of quinoa
(Chenopodium quinea Wild.) as a potential cash
crop halophyte. Australian Journal of Crop Science,
6 (2): 357-368.
- Fazeli, F., Ghorbanlı, M., Niknam, V., 2007. Effect of
drought on biomass, protein content, lipid
peroxidation and antioxidant enzymes in two sesame
cultivars. Biologia Plantarum, 51: 98-103.
- FAO, 2016. Food and Agriculture Organization of the
United Nations. Statistics Division.
http://www.fao.org/faostat/en/#data/QC( Erişim
tarihi: 06.10.2016).
- Foyer, C.H., Lelandais, M., Kunert, K.J., 1994.
Photooxidative stress in plants. Physiologia
Plantarum, 92 (4): 696-717.
- Gonzalez, K., Erdei L., Lips, H., 2002. The activity of
antioxidant enzymes in maize and sunflower
seedlings as affected by salinity and different
nitrogen sources. Plant Science, 162 (6): 923-930.
- Jalali-Emam, S.M.S., Alizadeh, B., Zaefizadeh, M.,
Zakarya, R.A., Khayatnezhad, M., 2011. Superoxide
dismutase (SOD) activity in NaCl stress in saltsensitive and salt-tolerance genotypes of Colza
(Brassica napus L.). Middle-East Journal of
Scientific Research, 7 (1): 7-11.
- Kaya, A., İnan, M., 2017. Tuz (NaCl) stresine maruz
kalan reyhan (Ocimum basilicum L.) bitkisinde bazı
morfolojik, fizyolojik ve biyokimyasal parametreler
üzerine salisilik asidin etkileri. Harran Tarım ve
Gıda Bilimleri Dergisi, 21 (3): 332-342.
- Kıran, S., Özkay, F., Kuşvuran, Ş., Ellialtıoğlu, Ş.Ş.,
2014. Tuz stresine tolerans seviyesi farklı domates
genotiplerinin kuraklık stresi koşullarında bazı
özelliklerinde meydana gelen değişimler.
Gaziosmanpaşa Üniversitesi Ziraat Fakültesi
Dergisi, 31(3): 41-48.
- Lhout, F.A., Zunzunegui, M., Barradas, M.C.D., Tirado,
R., Clavijo, A., Novo, F.G., 2001. Comparison of
proline accumulation in two mediterranean shrubs
subjected to natural and experimental water deficit.
Plant and Soil, 230: 175-183.
- Lopez, H., Marco, A., Ulery, A.P., Zohrab, S.,
Picchioni, G., Flynn, R.P., 2011. Response of chili
pepper (Capsicum annuum L.) to salt stress and
organic and inorganic nitrogen sources: I Growth
and yield. Tropical and Subtropical Agroecosystems,
14 (1): 137-147.
- Mafakheri, A., Siosemardeh, A., Bahramnejad, B.,
Struik, P.C., Sohrabi, Y., 2010. Effect of drought
stress on yield, proline and chlorophyll contents in
three chickpea cultivars. Australian Journal of Crop
Science, 4 (8): 580-585.
- Merchant, A., Tausz, M., Arndt, S.K., Adams, M.A.,
2006. Cyclitols and carbohydrates in leaves and
roots of 13 Eucalyptus species suggest contrasting
physiological responses to water deficit. Plant, Cell
& Environment, 29 (11): 2017–2029.
- Moran, J.F., Becana, M., Iturbe-Ormaetxe, I., Frechilla,
S., Klucas, R.V., Aparicio-Tejo, P., 1994. Drought
induces oxidative stres in pea plants. Planta, 194:
346-352.
- Ohkawa, H., Ohishi, N., Yagi, K., 1979. Assay for lipid
peroxides in animal tissues by thiobarbituric acid
reaction. Analytical Biochemistry, 95: 351- 358.
- Özkorkmaz, F., Yılmaz, N., 2017. Farklı tuz
konsantrasyonlarının fasulye (Phaseolus vulgaris L.)
ve börülcede (Vigna unguiculata L.) çimlenme
üzerine etkilerinin belirlenmesi. Ordu Üniversitesi
Bilim ve Teknoloji Dergisi, 7 (2): 196-200.
- Parmar, N., Singh, K. H., Sharma, D., Singh, L., Kumar,
P., Nanjundan, J., Khan, Y. J., Chauhan, D. K.,
Thakur, A.K., 2017. Genetic engineering strategies
for biotic and abiotic stress tolerance and quality
enhancement in horticultural crops: A
comprehensive review. 3 Biotech, 7 (4): 239-246.
- Penella, C., Landi, M., Guidi L., Nebauer, S.G.,
Pellegrini, E., San Bautista, A., Remorini, D., Nali,
C., Lopez-Galarza S., Calatayud, A., 2016. Salttolerant rootstock increases yield of pepper under
salinity through maintenance of photosynthetic
performance and sinks strength. Journal of Plant
Physiology, 193: 1-11.
- Sies, H., 2017. Hydrogen peroxide as a central redox
signaling molecule in physiological oxidative stress:
oxidative eustress. Redox Biology, 11: 613–619.
- Silva, J.R., Chaves, T.P., da Silva, A.R., Barbosa,
L.D.F., Costa, J.F., Ramos-Sobrinho, R., Teixeira,
R.R., Silva, S.J., Lima, G.S., Assunção, I.P., 2017.
- Molecular and morpho-cultural characterization of
Colletotrichum spp. associated with anthracnose on
Capsicum spp. in northeastern Brazil. Tropical Plant
Pathology, 42 (4): 315-319.
- Solmaz, I., Sarı, N., Dasgan Y., Aktaş, H., Yetişir, H.,
Ünlü, H., 2011. The effect of salinity on stomata and
leaf characteristics of dihaploid melon lines and their
hybrids. Journal of Food, Agriculture and
Environment, 9 (3-4): 172-176.
- Sreenivasulu, N., Grimm, B., Wobus, U., Weschke, W.,
2000. Differential response of antioxidant
compounds to salinity stress in salt-tolerant and saltsensitive seedling of fox-tail millet (Setaria italica).
Physiologia Plantarum, 109 (4): 435-442.
- Szabados, L., Savoure, A., 2010. Proline: a
multifunctional amino acid. Trends in Plant Science.
15(2): 89-97.
- Türkmen, Ö., Şensoy, S., Erdal, İ.,Kabay, T., (2002).
Kalsiyum uygulamalarının tuzlu fide yetiştirme
ortamlarında domateste çıkış ve fide gelişimi üzerine
etkileri. Yüzüncü Yıl Üniversitesi Tarım Bilimleri
Dergisi, 12 (2): 53-57.
- Ünyayar, S., Keleş, Y., Çekiç, F.Ö., 2005. The
antioxidative response of two tomato species with
different drought tolerances as a result of drought
and cadmium stress combinations. Plant Soil
Environment, 51: 57-64.
- Ünyayar, S., Keleş, Y., Ünal, E., 2004. Proline and
ABA levels in two sunflower genotypes subjected to
water stress. Bulgarian Journal of Plant Physiology,
30 (3-4): 34-47.
- Velikova, V., Yordanov, I., Edreva, A., 2000. Oxidative
stress and some antioxidant systems in acid raintreated bean plants, protective role of exogenous
polyamines. Plant Science, 151: 59–66.
- Vendruscolo, E.C.G., Schuster, I., Pileggi, M., Scapimd,
C.A., Molinarie, H.B.C., Marure, C.J., Vieira,
L.G.E., 2007. Stress-induced synthesis of proline
confers tolerance to water deficit in transgenic
wheat. Journal of Plant Physiology, 164: 1367-1376.
- Yemiş O, Bakkalbaşı E, Artık, N., (2004). Kapsaisinoit
kaynağı olarak kırmızıbiberler. Gıda Mühendisliği
Dergisi, 18: 30-37.
- Yu, Y., Assmann, S.M., 2016. The effect of NaCl on
stomatal opening in Arabidopsis wild type and agb1
heterotrimeric G-protein mutant plants. Plant
Signaling & Behavior, 11: e1085275.
- Zarcinas, B.A., Cartwright, B., Spouncer, L.R., 1987.
Nitric acid digestion and multi‐element analysis of
plant material by inductively coupled plasma
spectrometry. Communications in Soil Science and
Plant Analysis, 18 (1): 131-146.
- ZhongQun, H., ChaoXin H., ZhiBin Z., ZhiRong Z.,
HuaiSong W., 2007. Changes of antioxidative
enzymes and cell membrane osmosis in tomato
colonized by arbuscular mycorrhizae under NaCl
stress. Colloids and Surfaces B: Biointerfaces, 59:
128–133.