Bacillus thuringiensis subsp. kurstaki tarafından enfekte edilen Malacosoma neustria larvalarının hayatta kalmasına ve bazı bağışıklık enzimlerine bitkideki metal iyonlarının etkisi

Bu çalışmanın amacı 4 farklı bitki türü Elaeagnus rhamnoides, Quercus cerris, Coryllus maxima ve Crataegus monogyna ile besleyerek Bacillus thuringiensis subsp. kurstaki tarafından enfekte edilen Malacosoma neustria larvalarının hayatta kalmasına ve bazı bağışıklık enzimlerine bitkilerdeki krom, nikel, bakır ve çinko metal iyonlarının etkisini araştırmaktır. Düşük nikel miktarına sahip Q. cerris ve C. monogyna bitkileriyle beslenen larvalarda hayatta kalma oranı yüksek nikel miktarına sahip olan E. rhamnoides ve C. maxima’ya göre daha düşüktür. En yüksek bakır miktarına sahip Q. cerris bitkisi ile beslenen larvalarda katalaz enzimi diğer bitkilerle beslenen larvalara göre yüksektir. En yüksek çinko ve en düşük nikel miktarına sahip olan C. monogyna ile beslenen larvalarda glutatyon peroksidaz aktivitesi en yüksektir. Düşük çinko ve yüksek nikel miktarına sahip olan E. rhamnoides ile beslenen larvalarda en düşük glutatyon peroksidaz aktivitesi bulunmuştur. En yüksek çinko içeren C. monogyna ile beslenen enfekte edilmiş larvalarda süperoksit dismutaz aktivitesi en yüksektir. Yüksek nikel ve bakır miktarına sahip E. rhamnoides ile beslenen larvalarda hemosit sayısı en yüksektir. En düşük hemosit sayısı ise nikel ve bakır miktarının en az olduğu C. monogyna bitkisi ile beslenen larvalarda bulunmuştur.

The Effects of some metal ions in some plants on the survival and some ımmune enzymes of Malacosoma neustria larvae infected by Bacillus thuringiensis subsp. kurstaki

In this study, Malacosoma neustria larvae infected by Bacillus thuringiensis subsp. kurstaki were fed with 4 different types of plant species, Elaeagnus rhamnoides, Quercus cerris, Coryllus maxima and Crataegus monogyna, in order to see the effects of chromium, nickel, copper and zinc heavy metal ions in plants on the survival and the immune enzymes of these larvae. The larvae fed the C. monogyna plant with the highest zinc content had the lowest survival rate. In the infected groups, the catalase enzyme activity was the highest in larvae fed with the Q. cerris plant, which has a high chromium content, and the lowest in the larvae fed with E. rhamnoides. In the larvae fed with the highest copper-containing Q. cerris plant, the catalase enzyme was higher than the larvae fed with other plants. The larvae fed with C. monogyna with the highest zinc content had the highest glutathione peroxidase activity. The lowest glutathione peroxidase activity was found in the larvae fed with E. rhamnoides having low zinc content. Superoxide dismutase activity was the highest in the infected larvae fed with the highest zinc containing C. monogyna. The highest number of hemocytes were found in the larvae fed with E. rhamnoides containing high amounts of nickel and copper, while the lowest hemocyte count was found in larvae fed with C. monogyna plant, where the amount of nickel and copper was the lowest.

Keywords:

Malacosoma neustria, Metal ions, Plant Bacillus thuringiensis subsp. kurstaki,

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