0900 Ziraat kiraz çeşidinin sürgün ve yapraklarında fenolik madde içeriklerinin mevsimsel değişimlerinin saptanması

Bu çalışmada 0900 Ziraat kiraz çeşidinin yaprak ve sürgünlerinde toplam fenolik madde içeriklerinin mevsimsel değişimi saptanmıştır. Ayrıca yaprak ve sürgünlerdeki sulu alkolde çözünen ve asitle hidrolizi sonucu elde edilen ekstraktlarındaki fenolik maddeler kağıt kromatografısi ile ayrılarak incelenmiştir. Sürgünlerde toplam fenolik madde içerikleri yapraklara göre daha yüksek oranlarda saptanmıştır. Sürgünlerin toplam fenotik içeriklerinin, Nisan ve Mayıs aylarında göreceli olarak düşük seviyede olduğu, Haziranda ani bir artış gösterdiği, hemen ardından Temmuz ayında yılın en düşük seviyesine indiği gözlenmiş; Temmuzdan sonra Ağustos ayında ise tekrar yükseldiği tespit edilmiştir. Daha sonra Eylül - Mart ayları arasında (dinlenme döneminde) diğer aylara göre yüksek oranlarda olduğu belirlenmiştir. Ya'praklardaki toplam fenolik maddeler, yaprakların açmaya başladığı Nisan ayının ilk haftasında, diğer dönemlere göre en yüksek oranda belirlenmiştir. Haziran ve Ağustos ayları arasında oldukça düşük seviyelerde seyreden yaprak fenolik maddeleri, yaprakların vegetasyonlarmı tamamladıkları Eylül ve Ekim aylarında vegetasyon başındaki kadar olmasa da tekrar yükselmişlerdir. Sürgünlerin sulu alkol ekstraktlannda iki boyutlu kağıt kromatografisiyle (birinci boyut %5 asetik asit; ikinci boyut n- butanol: asetik asit: su, 60:15:25 v/v) toplam 12 farklı madde saptanmıştır. Aynı ayırma metoduyla yapraklarda toplam 8 farklı madde elde edilmiştir. Sürgünlerin ve yaprakların forestal solvent! ile yapılan tek boyutlu kağıt kromatogramlarında 5' er farklı madde gözlenmiştir.

Anahtar Kelimeler:

kiraz, fenolik bileşikler, mevsimsel değişimler, yapraklar, filiz, fitokimyasallar

A research on determination the seasonal variation in phenolic constituents of 0900 Ziraat cherry CV. leavesand shoots

In this research, the seasonal variation in total phenolic contents in 0900 Ziraat sweet cherry cv. leaves and shoots was determined. In addition, the phenolic compounds extracted by aqueous alcohol or obtained by acid hydrolysis of the leaves and shoots were Separated by paper chromatography (PC). It was found that in general, the total phenolic contents of the shoots were higher than the leaves. The total phenolic contents of the shoots in April and may were found to be relatively lower; but a sudden increase was observed in June; the lowest total phenolic content of the shoots was observed in July; in August, it started to rise again. Then, from September through March (during the resting period), the phenolic contents were relavitely higher than the other months in shoots. The total phenolic content of the leaves was at its highest level in the first week of april when the leaves were begun to grow up comparing to the rest of the year. During June through August, the phenolic contents of the leaves were stayed relatively higher levels; but at the end of the vegatative season during September and October, it was rose again to a higher level similar to that of the beginning of the vegetative season. The 0900 Ziraat cherry cv. shoots aqueous extracts gave a total of 12 different compounds when separated by two dimensjol PC (5% asetic acid on the first dimension, n-butanol: acetic acid: water on the second dimension). When the leaf extracts were separated by the same technique, a total of 4 different compounds were obtained on the chromatograms. One dimensiol chromatograms obtained by forestal solvent showed that were 5 different compounds separated from shoots and leaves.

Keywords:

cherries, phenolic compounds, seasonal variation, leaves, shoots, phytochemicals,

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Anonim, 2003a. http://www.fao.org
Anonim, 2003b. http://www.tarim.gov.tr
Bate-Smith, E.C. and Metcalfe, C.R. 1957. Leucoanthociyanins. 3. The Nature and Systematic Distribution of Tannins in Dicotyledonous Plants. J. Liin. Soc. of London Botany, LV 362:669-705.
Bate- Smith, J.B. 1962. The Phenolic Constituents of Plants and Their Taxonomic Significance 1.Dicotyledons.J. Linn.Soc.(Bot),58,371:95-173.
Bilgener, M. 1988.Chemical Components of Howler Monkey (Alouatta palliata) Food Choice and Kinetics of Tanin Binding with Natural Polymers. Ph. D. Dissertation, Boston University.
Bilgener, K.Ş. 1999. Fındık (Coryllus avellana L.) ve Kestane (Castanea sativa Mill.) Yaprak ve Sürgünlerinde Fenolik madde içeriklerinin Mevsimsel değişimleri. Tr. J. of Agriculture and Forestry 23 : 5(ek sayı), 1215-1221.
Camm, El, Towers , G.H.N. 1973. Review Article Phenylalanine Ammonia Lyase. Phytochemistry 12:961 -973. Pergamon Press. England.
Cooper - Driver G.A., Finch. S., Swain. T. 1977. Seasonal Variation in Secondary Plant Compounds in Relation to Palatability of Pteridium Aqulium. Biocem.,Syst. And Ecol., 5: 177-183.
Ellis, B.E. 1997. Metabolism of Defence and Communication, pp. 148-160.-In: DENNIS D.T, D.H. TURPIN, D.D. LEFEBVRE, and D.B. LAYZELL (eds.) Plant Metabolism. Longman,Signapore,2 'nd edition.
Errea, P., 1997. Implications Of Phenolic Compounds In Graft Incompatibility In Fruit Tree Species. Scientia Horticulturae 74: 195-205
Feeny, P.P. Bostock, H. 1968. Seasonal Changes in The Tannin Content of Oak Leaves. Phytochemistry, 7: 871 - 880.
Feucht W., Treutter, D.Keukenkamp I. 1998. Growth enhancement of grapevine callus by catechin on auxin- free media. Vitis, 37:67-71.
Harborne, J.B. 1972. (ed).Phytochemical Ecology, Academic Press,New York.
Harborne, J.B. 1974. Phytochemical Methods, Chapman & Hall, London.
Harborne, J.B. 1979. Flavonoid Pigments, In Herbivors: Their Interactions With Secondary Metabolites, Rosenthal, G.A. and D.H. Janzen (Eds), 619 - 656, Academic Press, New York.
Harborne, J.B. 1980. Plant Phenolics. -In Secondary Plant Products. (E. A. Bell and B. V. Charlwood. Ed. Pp.329-402. Springer- Verlag, Berlin. ISBN 3-540-09461-X.
Haukioja, E., Niemela. P. 1979. Birc Leaves as a Resource for Herbivores: Seasonal Occurrence of Increased Resistance in Foliage After Mechanical Damage of Adjacent Leaves. Oecologia. 39:151-159.
Haukioja, E. 1990.1nduction of Defenses in Trees. Ann. Rev. Entomol.36:25-42.
Hedin, P.A. ; Waage, S.K. 1986. Roles of Flavonoids in Plant Resistance to Insect Plant Flavonoids in Biology and Medicine, Biochemical Pharmacological and Structure - Activity Relationships, Alan R. Liss, New York.
Jones, D.H. 1984. Phenylalanine Ammonia Lyase: Regulation of It's Induction, and Its Role in the Plant Development. Phytochemistry 23:1349-1359
Kefeli, V.I.; Kadyrov, C.S. 1971. Natural Growth Inhibitors Their Chemical and Physiological Properties. Annu. Rev. Plant Physio. 22: 185 -196.
Lamb, C.J.; Lawton, M.A.; Dron, M. 1989. Dixon, R.A., Signals and Transduction Mechanism for Activation of Defenses Against Microbial Attack. Cell, 56:215.
Marks, D.L. 1985. Ecological Chemistry of Primate Food Choice: II - Comparative Methods in Ecological Chemistry, Ph.D. Dissertation. Boston University, Boston.
Özbek, S., 1978. Özel Meyvecilik. Ç.Ü. Zir. Fak. Yayınları: 128,486 s.
Özeker, E., Tannsever, A. 1995. Bazı Çilek Çeşitlerinin Farklı Organlarındaki Fenolik Maddeler Üzerinde Araştırmalar. Ege Üniv, Ziraat Fak. Dergisi, 32 (3): 9-16.
Rhoades, D.F., Cates, R.G. 1976. Toward a General Theory of Plant Antiherbivory Chemistry, in Advances in Phytochemistry 10, J. W. Wllace and R.L. Mansell. Eds., Plenum Press, Newyork
Rhoades, D.F. 1979. Evolution of Plant Chemical Defense Against Herbivores, inHerbivores, G.A. Rosenthal and Janzen, edş., 1-47, Academic Press, Newyork.
Rodriquez, A.; Canal, M.J. 1988. Tames, R.S., Indoleacetic Acid, Absisic Acid and Phenolic Substances During Development of Hazel Leaves, Physiologia Plantarum 73: 92 - 96. Copenhagen.
Swain, T.; Hillis, W.E. 1959. The Phenolic Constituents of Prunus domestica. J. Sci. Food Agric, 10: 63-68.
Swain, T. 1977. Secondary Compounds as Protective Agents, Ann. Rev. Plant Physiol., 28: 479-501.
Tena ,M., Lopez - Valbuena R., JorrinJ. 1984. Induction of Phenylalanine Ammonia ~ Lyase in Hypocotyls of Sunflower seedlings by Light, Excision and Sucrose. -Physiol. Plant., 60:159-165.
Whittaker, R.H., Feeny P.P. 1971. Allelochemics : /Chemical Interactions Between Species, Science, 171:757-769.