Habib DASTMALCHI SAEI, Mohammad ZAVARSHANI

Phylogenetic Grouping of Verotoxigenic Escherichia coli (VTEC) Obtained from Sheep and Broiler Chicken in Northwestern Iran

Verotoxigenic Escherichia coli (VTEC) are major foodborne pathogens with an increasing public health concern. The purpose of this study was to investigate the occurrence and the phylogenetic groups of VTEC isolates from the feces of healthy sheep and broiler chickens at a slaughterhouse in Urmia region, Northwestern Iran. A total of 446 E. coli isolates (97 from sheep and 349 from broiler chickens) were assessed for the occurrence of the Vtx-encoding genes (vtx1 and vtx2) using polymerase chain reaction. Then, all the recovered VTEC isolates were phylogenetically grouped based on the Clermont phylotyping method using three genetic sequences, the so-called chuA, yjaA, and TSPE4.C2. The vtx gene-carrying E. coli was identified in 46.4% (45/97) of sheep-originated isolates and in 8.3% (29/349) of broiler chicken-originated isolates. In general, phylotyping revealed that 74 VTEC isolates segregated in the phylogenetic groups A (32.4%; designated as VTEC-A), B1 (44.6%; VTEC-B1), B2 (9.5%; VTEC-B2), and D (13.5%; VTEC-D). The results also showed that the dissemination of VTEC isolates of sheep and broiler chicken origin varied noticeably in their assignment to B1 and D phylogenetic groups (p

Kuzeybatı İran'da Koyun ve Broyler Tavuğundan Elde Edilen Verotoksijenik Escherichia coli (VTEC) Filogenetik Grubu

Verotoksijenik Escherichia coli (VTEC), artan bir halk sağlığı sorunu olan başlıca gıda kaynaklı patojenlerdir. Çalışmanın amacı, Kuzeybatı İran’ın Urmia bölgesinde kesilen sağlıklı koyun ve broyler tavuklarının dışkılarından VTEC izolatlarının oluşum ve filogenetik gruplarını araştırmaktır. Bu çalışmada, 446 E. coli izolatı (koyundan 97 ve broyler tavuktarından 349), polimeraz zincir reaksiyonu (PCR) kullanılarak Vtx-kodlayan genlerin (vtx1 ve vtx2) oluşumu için test edilmiştir. Daha sonra, tüm geri kazanılmış VTEC izolatları, chuA, yjaA ve TSPE4.C2 olarak adlandırılan üç genetik sekans kullanılarak Clermont filotiplemeye dayanan filogenetik olarak gruplandırılmıştır. Sonuç olarak, koyun kaynaklı izolatların %46,4’ünde (45/97) vtx geni taşıyan E. coli saptanmış ve broylerler için saptanan yüzdeler ise %8,3 (29/349) olmuştur. Genel olarak, filotipleme, 74 VTEC izolatının, filogenetik olarak grup A (%32,4, VTEC-A olarak belirlenmiş), B1 (%44,6; VTEC-B1), B2 (%9,5; VTEC-B2) ve D (%13,5; VTEC-D) olarak ayrıldığını ortaya koymuştur. Buna ek olarak, sonuçlar ayrıca, koyun ve broyler tavuklarının VTEC izolatlarının yayılmasının B1 ve D filogenetik grupları arasında belirgin olarak farklılık gösterdiğini göstermiştir (p

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