Afrikalı Yayın Balığı (Clarias gariepinus) içinde Demirhindi (Tamarindus indica) Selüloz ve Yaprak Öğünlerinin Yara İyileşmesi ve Antioksidan Aktivitelerinin Deneysel Olarak Değerlendirilmesi
Tamarindus indica’nın meyveleri ve yaprakları, Afrika’daki yaraların ve diğer hastalıkların tedavisi için geleneksel tıpta yaygın olarak kullanılmaktadır. Bu çalışmada, T. indica posası (TP) ve yaprak (TL) öğünün yara iyileşme özelliği ve Clarias gariepinus’ta yara iyileşme sürecindeki antioksidan enzimlerin önemi araştırılmıştır. Lateral kısımda, pelvik yüzgecin üzerinde ve kaudal bölgeye doğru aseptik olarak 10 mm2 ’lik cerrahi insizyonlar yapılmıştır. Balıklar, her biri TP veya TL ile takviye edilmiş bazal diyetle %0,5, %1,0, %1,5, %2,0 ve %0,0 (müdahale edilmeyen) ve %0,2 oksitetrasiklin (müdahale edilen) kontrol grubu olarak üç grup halinde beslenmiştir. Yara bölgesindeki değişiklikler (mm2 ) on beş gün boyunca üç gün aralıklarla ölçülmüştür. İyileşme oranları ve nispi yüzde iyileşmeleri hesaplanmıştır. Deneysel balıklardan alınan serumlar oksidatif stres biyobelirteçleri ve antioksidan enzimler için analiz edilmiştir. Sonuçlar, TP ve TL ile beslenen balıkların, kontrol gruplarına kıyasla, 6. ve 12. günlerde, lateral ve kaudal bölgelerdeki günlük iyileşme oranını anlamlı derecede daha hızlı (p=0,05) olduğunu göstermiştir. Lateral ve kaudal bölgelerdeki Yara-İyileşme Yüzdesi (PWH), demirhindi ile tedavi edilen gruplarda 6. günden itibaren önemli ölçüde artmıştır (p=0,05). Tüm TL ve %1,5-2,0 TP diyetleri için PWH 12. günde lateral bölgede doruğa (%100) ulaşmıştır. TP ve TL ile yapılan diyet tedavisi, düşük serum glutatyon, süperoksit dismutaz ve glutatyon peroksidazını arttırırken, serum malondialdehit ve hidrojen peroksidin daha az üretilmesine neden olmuştur. %1,0-2,0 oranında demirhindi küspesi ve yaprak unu ile C. gariepinus takviye edici diyetler, doğal iyileşme ve oksietrasiklin takviyeli diyete nispeten önemli ölçüde yara iyileşmesini arttırmıştır. Daha hızlı yara iyileşmesi, balıklarda demirhindi ile takviye edilmiş diyetlerle beslenen serum antioksidanlarının yüksek olmasının bir sonucu olabilir.
Experimental Evaluation of the Wound-healing and Antioxidant Activities of Tamarind (Tamarindus indica) Pulp and Leaf Meal in the African Catfish (Clarias gariepinus)
The fruits and leaves of Tamarindus indica have been widelyused in traditional medicine for treating wounds and otherdiseases in Africa. The aim of this study was to investigatethe wound-healing property of T. indica pulp (TP) and leaf(TL) meal and the importance of antioxidant enzymes in thewound-healing process in the African catfish, Clarias gariepinus. Surgical incisions of 10 mm2 were made aseptically onthe lateral part of the catfish, above the pelvic fin and towardthe caudal region. The fish were fed experimental diets composed of basal diet fortified with each of TP or TL at concentrations of 0.5%, 1.0%, 1.5%, 2.0%, and 0.0% (untreated) and0.2% oxytetracycline (treated) as controls in triplicate groups.Changes occurring in the wound area (mm2) were measuredat 3-day-intervals for 15 days. The healing rates and the relative percentage of healing were calculated. Sera collectedfrom the experimental fish were analyzed for oxidative stressbiomarkers and antioxidant enzymes. The results showedthat fish fed diets treated with TP or TL had significantly faster(p
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