Kuzeybatı İran'da Koyun ve Broyler Tavuğundan Elde Edilen Verotoksijenik Escherichia coli (VTEC) Filogenetik Grubu
Verotoksijenik Escherichia coli (VTEC), artan bir halk sağlığı sorunu olan başlıca gıda kaynaklı patojenlerdir. Çalışmanın amacı, Kuzeybatı İran’ın Urmia bölgesinde kesilen sağlıklı koyun ve broyler tavuklarının dışkılarından VTEC izolatlarının oluşum ve filogenetik gruplarını araştırmaktır. Bu çalışmada, 446 E. coli izolatı (koyundan 97 ve broyler tavuktarından 349), polimeraz zincir reaksiyonu (PCR) kullanılarak Vtx-kodlayan genlerin (vtx1 ve vtx2) oluşumu için test edilmiştir. Daha sonra, tüm geri kazanılmış VTEC izolatları, chuA, yjaA ve TSPE4.C2 olarak adlandırılan üç genetik sekans kullanılarak Clermont filotiplemeye dayanan filogenetik olarak gruplandırılmıştır. Sonuç olarak, koyun kaynaklı izolatların %46,4’ünde (45/97) vtx geni taşıyan E. coli saptanmış ve broylerler için saptanan yüzdeler ise %8,3 (29/349) olmuştur. Genel olarak, filotipleme, 74 VTEC izolatının, filogenetik olarak grup A (%32,4, VTEC-A olarak belirlenmiş), B1 (%44,6; VTEC-B1), B2 (%9,5; VTEC-B2) ve D (%13,5; VTEC-D) olarak ayrıldığını ortaya koymuştur. Buna ek olarak, sonuçlar ayrıca, koyun ve broyler tavuklarının VTEC izolatlarının yayılmasının B1 ve D filogenetik grupları arasında belirgin olarak farklılık gösterdiğini göstermiştir (p
Phylogenetic Grouping of Verotoxigenic Escherichia coli (VTEC) Obtained from Sheep and Broiler Chicken in Northwestern Iran
Verotoxigenic Escherichia coli (VTEC) are major foodborne pathogens with an increasing public health concern. The purposeof this study was to investigate the occurrence and the phylogenetic groups of VTEC isolates from the feces of healthy sheepand broiler chickens at a slaughterhouse in Urmia region, Northwestern Iran. A total of 446 E. coli isolates (97 from sheep and349 from broiler chickens) were assessed for the occurrence ofthe Vtx-encoding genes (vtx1 and vtx2) using polymerase chainreaction. Then, all the recovered VTEC isolates were phylogenetically grouped based on the Clermont phylotyping methodusing three genetic sequences, the so-called chuA, yjaA, andTSPE4.C2. The vtx gene-carrying E. coli was identified in 46.4%(45/97) of sheep-originated isolates and in 8.3% (29/349) of broiler chicken-originated isolates. In general, phylotyping revealed that 74 VTEC isolates segregated in the phylogenetic groupsA (32.4%; designated as VTEC-A), B1 (44.6%; VTEC-B1), B2 (9.5%;VTEC-B2), and D (13.5%; VTEC-D). The results also showed thatthe dissemination of VTEC isolates of sheep and broiler chickenorigin varied noticeably in their assignment to B1 and D phylogenetic groups (p
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- Alonso, M.Z., Lucchesi, P.M.A., Rodríguez, E.M., Parma, A.E., Padola, N.L., 2012. Enteropathogenic (EPEC) and Shigatoxigenic Escherichia coli (STEC) in broiler chickens and derived products at different
retail stores. Food Control 23(2), 351-355. [Crossref]
- Bagheri, M., Ghanbarpour, R., Alizade, H., 2014. Shiga toxin and beta-lactamases genes in Escherichia coli phylotypes isolated from carcasses of broiler chickens slaughtered in Iran. International Journal
of Food Microbiology 177, 16-20. [Crossref]
Bergthorsson, U., Ochman, H., 1998. Distribution of chromosome
length variation in natural isolates of Escherichia coli. Molecular Biology and Evolution 15(1), 6-16. [Crossref]
- Blanco, M., Blanco, J.E., Mora, A., Rey, J., Alonso, J.M., Hermoso, M.,
Hermoso, J., Alonso, M.P., Dahbi, G., Gonzalez, E.A., Bernardez, M.I.,
Blanco, J., 2003. Serotypes, virulence genes, and intimin types of Shiga
toxin (verotoxin)-producing Escherichia coli isolates from healthy sheep
in Spain. Journal of Clinical Microbiology 41(4), 1351-1356. [Crossref]
- Carlos, C., Pires, M.M., Stoppe, N.C., Hachich, E.M., Sato, M.I.,
Gomes, T.A., Amaral, L.A., Ottoboni, L.M., 2010. Escherichia coli
phylogenetic group determination and its application in the identification of the major animal source of fecal contamination. BMC
Microbiology 10, 161. [Crossref]
- Cho, S., Fossler, C.P., Diez-Gonzalez, F., Wells, S.J., Hedberg, C.W.,
Kaneene, J.B., Ruegg, P.L., Warnick, L.D., Bender, J.B., 2013.
Herd-level risk factors associated with fecal shedding of Shiga toxin-encoding bacteria on dairy farms in Minnesota, USA. Canadian
Veterinary Journal 54(7), 693-697.
- Clermont, O., Bonacorsi, S., Bingen, E., 2000. Rapid and simple determination of the Escherichia coli phylogenetic group. Applied and
Environmental Microbiology 66(10), 4555-4558. [Crossref]
- Clermont, O., Olier, M., Hoede, C., Diancourt, L., Brisse, S., Keroudean, M., Glodt, J., Picard, B., Oswald, E., Denamur, E., 2011.
Animal and human pathogenic Escherichia coli strains share common genetic backgrounds. Infection, Genetics and Evolution 11(3),
654-662. [Crossref]
- Dixit, S.M., Gordon, D.M., Wu, X.Y., Chapman, T., Kailasapathy, K.,
Chin, J.J., 2004. Diversity analysis of commensal porcine Escherichia coli - associations between genotypes and habitat in the porcine
gastrointestinal tract. Microbiology 150(Pt 6), 1735-1740. [Crossref]
- Escobar-Paramo, P., Grenet, K., Le Menac’h, A., Rode, L., Salgado,
E., Amorin, C., Gouriou, S., Picard, B., Rahimy, M.C., Andremont,
A., Denamur, E., Ruimy, R., 2004. Large-scale population structure
of human commensal Escherichia coli isolates. Applied and environmental microbiology 70(9), 5698-5700. [Crossref]
- Farooq, S., Hussain, I., Mir, M.A., Bhat, M.A., Wani, S.A., 2009. Isolation of atypical enteropathogenic Escherichia coli and Shiga toxin 1
and 2f-producing Escherichia coli from avian species in India. Letters
in Applied Microbiology 48(6), 692-697.]
- Garcia-Aljaro, C., Moreno, E., Andreu, A., Prats, G., Blanch, A.R.,
2009. Phylogroups, virulence determinants and antimicrobial resistance in stx(2) gene-carrying Escherichia coli isolated from aquatic
environments. Research in Microbiology 160(8), 585-591. [Crossref]
- Ghanbarpour, R., Kiani, M., 2013. Characterization of non-O157 shiga toxin-producing Escherichia coli isolates from healthy fat-tailed
sheep in southeastern of Iran. Tropical Animal Health and Production
45(2), 641-648. [Crossref]
- Ghanbarpour, R., Sami, M., Salehi, M., Ouromiei, M., 2011. Phylogenetic background and virulence genes of Escherichia coli isolates
from colisepticemic and healthy broiler chickens in Iran. Tropical Animal Health and Production 43(1), 153-157. [Crossref]
- Girardeau, J.P., Dalmasso, A., Bertin, Y., Ducrot, C., Bord, S., Livrelli,
V., Vernozy-Rozand, C., Martin, C., 2005. Association of virulence
genotype with phylogenetic background in comparison to different
seropathotypes of Shiga toxin-producing Escherichia coli isolates.
Journal of Clinical Microbiology 43(12), 6098-6107. [Crossref]
- Gordon, D.M., Clermont, O., Tolley, H., Denamur, E., 2008. Assigning
Escherichia coli strains to phylogenetic groups: multi-locus sequence
typing versus the PCR triplex method. Environmental Microbiology
10(10), 2484-2496. [Crossref]
- Gyles, C.L., 2007. Shiga toxin-producing Escherichia coli: an overview.
Journal of Animal Science 85(13 Suppl), E45-62. [Crossref]
- Higgins, J., Hohn, C., Hornor, S., Frana, M., Denver, M., Joerger, R.,
2007. Genotyping of Escherichia coli from environmental and animal
samples. Journal of Microbiological Methods 70(2), 227-235. [Crossref]
- Horcajo, P., Dominguez-Bernal, G., De La Fuente, R., Ruiz-Santa-Quiteria, J.A., Orden, J.A., 2010. Association of vt1c with verotoxin-producing Escherichia coli from goats and sheep. Journal of
Veterinary Diagnostic Investigation 22(2), 332-334. [Crossref]
- Ishii, S., Meyer, K.P., Sadowsky, M.J., 2007. Relationship between phylogenetic groups, genotypic clusters, and virulence gene profiles of
Escherichia coli strains from diverse human and animal sources. Applied and Environmental Microbiology 73(18), 5703-5710. [Crossref]
- Kaper, J.B., Nataro, J.P., Mobley, H.L., 2004. Pathogenic Escherichia
coli. Nature Reviews Microbiology 2, 123-140. [Crossref]
- Maluta, R.P., Fairbrother, J.M., Stella, A.E., Rigobelo, E.C., Martinez,
R., de Avila, F.A., 2014. Potentially pathogenic Escherichia coli in
healthy, pasture-raised sheep on farms and at the abattoir in Brazil.
Veterinary Microbiology 169(1-2), 89-95. [Crossref]
- Martins, R.P., da Silva, M.C., Dutra, V., Nakazato, L., Leite Dda, S.,
2013. Preliminary virulence genotyping and phylogeny of Escherichia coli from the gut of pigs at slaughtering stage in Brazil. Meat
Science 93(3), 437-440. [Crossref]
- Mora, A., Herrera, A., Mamani, R., Lopez, C., Alonso, M.P., Blanco,
J.E., Blanco, M., Dahbi, G., Garcia-Garrote, F., Pita, J.M., Coira,
A., Bernardez, M.I., Blanco, J., 2010. Recent emergence of clonal group O25b:K1:H4-B2-ST131 ibeA strains among Escherichia coli
poultry isolates, including CTX-M-9-producing strains, and comparison with clinical human isolates. Applied and Environmental Microbiology 76(21), 6991-6997. [Crossref]
- Mora, A., Viso, S., Lopez, C., Alonso, M.P., Garcia-Garrote, F., Dabhi,
G., Mamani, R., Herrera, A., Marzoa, J., Blanco, M., Blanco, J.E.,
Moulin-Schouleur, M., Schouler, C., Blanco, J., 2013. Poultry as
reservoir for extraintestinal pathogenic Escherichia coli O45:K1:H7-B2-
ST95 in humans. Veterinary Microbiology 167(3-4), 506-512. [Crossref]
- Navidinia, M., Peerayeh, S.N., Fallah, F., Bakhshi, B., 2013. Phylogenetic Groups and Pathogenicity Island Markers in Escherichia coli
Isolated From Children. Jundishapur Journal of Microbiology 6(10),
e8362. [Crossref]
- Novais, A., Vuotto, C., Pires, J., Montenegro, C., Donelli, G., Coque,
T.M., Peixe, L., 2013. Diversity and biofilm-production ability among
isolates of Escherichia coli phylogroup D belonging to ST69, ST393
and ST405 clonal groups. BMC Microbiology 13, 144. [Crossref]
- Nowrouzian, F.L., Wold, A.E., Adlerberth, I., 2005. Escherichia coli
strains belonging to phylogenetic group B2 have superior capacity to persist in the intestinal microflora of infants. The Journal of Infectious Disease 191(7), 1078-1083. [Crossref]
- Obeng, A.S., Rickard, H., Ndi, O., Sexton, M., Barton, M., 2012. Antibiotic resistance, phylogenetic grouping and virulence potential of
Escherichia coli isolated from the faeces of intensively farmed and free
range poultry. Veterinary Microbiology 154(3-4), 305-315. [Crossref]
- Oporto, B., Esteban, J.I., Aduriz, G., Juste, R.A., Hurtado, A., 2008.
Escherichia coli O157:H7 and non-O157 Shiga toxin-producing E. coli
in healthy cattle, sheep and swine herds in Northern Spain. Zoonoses Public Health 55(2), 73-81. [Crossref]
- Osek, J., 2003. Development of a multiplex PCR approach for the identification of Shiga toxin-producing Escherichia coli strains and their
major virulence factor genes. Journal of Applied Microbiology 95(6),
1217-1225. [Crossref]
- Paton, A.W., Paton, J.C., 2002. Direct detection and characterization of
Shiga toxigenic Escherichia coli by multiplex PCR for stx1, stx2, eae, ehxA,
and saa. Journal of Clinical Microbiology 40(1), 271-274. [Crossref]
- Ramazanzadeh, R., Zamani, S., Zamani, S., 2013. Genetic diversity in
clinical isolates of Escherichia coli by enterobacterial repetitive intergenic consensus (ERIC)-PCR technique in Sanandaj hospitals. Iranian
Journal of Microbiology 5(2), 126-131.
- Rey, J., Blanco, J.E., Blanco, M., Mora, A., Dahbi, G., Alonso, J.M.,
Hermoso, M., Hermoso, J., Alonso, M.P., Usera, M.A., Gonzalez,
E.A., Bernardez, M.I., Blanco, J., 2003. Serotypes, phage types and
virulence genes of shiga-producing Escherichia coli isolated from
sheep in Spain. Veterinary Microbiology 94(1), 47-56. [Crossref]
- Riffon, R., Sayasith, K., Khalil, H., Dubreuil, P., Drolet, M., Lagace, J.,
2001. Development of a rapid and sensitive test for identification of
major pathogens in bovine mastitis by PCR. Journal of Clinical Microbiology 39(7), 2584-2589. [Crossref]
- Saei, H.D., Ayremlou, N., 2012. Characterization of Shiga toxin-producing Escherichia coli (STEC) in feces of healthy and diarrheic calves
in Urmia region, Iran. Iranian Journal of Microbiology 4(2), 63-69.
- Salehi, M., 2014. Determination of intimin and Shiga toxin genes in
Escherichia coli isolates from gastrointestinal contents of healthy
broiler chickens in Kerman City, Iran. Comparative Clinical Pathology
23(1), 175-179. [Crossref]
- Tahamtan, Y., Hayati, M., Mehdi Namavari, M., 2010. Contamination of sheep carcasses with verocytotoxin producing Escherchia coli
during slaughtering. Transboundary and Emerging Diseases 57(1-2),
25-27. [Crossref]
- Unno, T., Han, D., Jang, J., Lee, S.N., Ko, G., Choi, H.Y., Kim, J.H., Sadowsky, M.J., Hur, H.G., 2009. Absence of Escherichia coli phylogenetic group B2 strains in humans and domesticated animals from
Jeonnam Province, Republic of Korea. Applied and Environmental
Microbiology 75(17), 5659-5666. [Crossref]
- Vettorato, M.P., de Castro, A.F., Cergole-Novella, M.C., Camargo,
F.L., Irino, K., Guth, B.E., 2009. Shiga toxin-producing Escherichia
coli and atypical enteropathogenic Escherichia coli strains isolated
from healthy sheep of different populations in Sao Paulo, Brazil. Letters in Applied Microbiology 49(1), 53-59. [Crossref]
- Wasteson, Y., 2001. Zoonotic Escherichia coli. Acta Veterinaria Scandinavica 95, 79-84.
- Zweifel, C., Zychowska, M.A., Stephan, R., 2004. Prevalence and
characteristics of Shiga toxin-producing Escherichia coli, Salmonella spp. and Campylobacter spp. isolated from slaughtered sheep
in Switzerland. International Journal of Food Microbiology 92(1),
45-53.[Crossref]