Effects of protein restriction on the live weight gain and some blood parametres in nature and ımmature rats

Bu çalışma, puberta öncesi ve puberta sonrası ratların rasyonlarına farklı oranlarda protein kısıtlamasının canlı ağırlık kazancı, yem tüketimi ve bazı kan parametreleri üzerine etkisini karşılaştırmak amacı ile yapıldı. Denek olarak , puberta öncesi grup 20 günlük ve puberta sonrası grup 65 günlük olacak şekilde 24’er adet erkek Wistar Albino rat seçildi. İki aylık deneme süresince, iki haftada bir her gruptaki ratların canlı ağırlık artışı ve yem tüketim miktarı belirlenerek kaydedildi. Boyundan alınan kan örneklerinde plazma total protein, glikoz ve kolesterol seviyeleri belirlendi. Dietteki CP konsantrasyonu arttıkça puberta öncesi ve puberta sonrası ratlarda hem canlı agırlık kazancı hem de yem tüketimi önemli derecede arttığı gözlendi (p0.05). Plazma total protein konsantrasyonu, hem puberta öncesi hem de puberta sonrası ratlarda diyetin protein içerigindeki artığa baglı olarak artmıstır (p

Puberta öncesi ve puberta sonrası ratlarda protein kısıtlamasının canlı ağırlık ve bazı kan parametreleri üzerine etkisi

The aim of this study was to compare the effects of different levels of protein restriction on live weight gain, feed intake and some blood parameters in immature and mature rats. Twenty &#8211; four 20 day-old (immature group) and twenty &#8211; four 65 day-old (mature group) male Wistar Albino rats were selected as the experimental subjects. All rats were weighed bi-weekly and amounts of feed consumed were recorded for each cages. Blood samples were collected from the neck and then, plasma total protein, glucose, and cholesterol levels were determined. As CP concentration of diets increased, both live weight gain and feed intake significantly increased in both mature and immature rats (P<0.05). Plasma glucose and cholesterol levels were similar among rats (P>0.05). Plasma total protein concentrations linearly increased as protein content of diets increased in both mature and immature rats (P<0.05). It can be concluded that immature rats can be affected from protein restriction more compared with mature rats based on growth performance of rats on protein-restricted diets.

___

1. Alkan M (2002): Proteinden yetersiz diyetle beslenen ratlarda serum lipid, sialik asit ve vitamin E düzeylerinin araştırılması. PhD Thesis, Y.Y.U. Sağ. Bil. Enst., Van.

2. A.O.A.C. (1980): Official Methods of Analysis (13th Ed.). Association of Official Analytical Chemists, Washington DC

3. Baysal A (1999): Beslenme. Hatipoğlu Yayınevi, 8. Baskı, Ankara, 1999.

4. Bhattacaryya A K (1986): Protein – energy malnutrition (Kwashiorkor-Marasmus Syndrome); terminology, classification and evolution. World Rev. Nutr. Diet., 47, 80-133.

5. Bouziane M, Prost J, Belleville J (1994): Changes in fatty acid compositions of total serum and lipoprotein particles, in growing rats given proteindeficient diets with either hydrogenated coconut or salmon oils as fat sources. Br. J. Nutr., 71, 375-387.

6. Bydlowski S P, Stivaletti V L G, Douglas C R (1986): Biochemical observations on rat aorta: interaction of dietary protein and cholesterol. Br. J. Nutr., 55, 295- 304.

7. Dülger H (1998): Van ve çevresinde yaşayan çocuklarda ve gençlerde açlık insulin seviyesi ile serum lipid ve lipoproteinleri arasında ilişkilerin araştırılması. PhD Thesis, Y.Y.U. Sag. Bil. Enst., Van.

8. Huang C H, Fwu M L (1993): Degree of protein deficiency affects the extent of the depression of the antioxidative enzyme activities and the enhancement of tissue lipid per oxidation in rats. J. Nutr., 123, 803-810.

9. Karaca F, Dönmez H H, Karsli M A (2003): Effects of protein deficiency on testosterone levels, semen quality and testicular histology in the developing male rat. Scand. J. Lab. Anim. Sci., 1(30), 7-13.

10. Kern M, Beuttenmuller D, Diehl S, Mccormick C, Ambrose M (2002): The effects of protein repletion at varied levels on the growth and nutritional status of protein restricted rats. Nutr. Research, 22, 957-963.

11. Konno A, Utsuyama M, Kurashima C, Kasai M, Kimura S, Hirokawa K (1993): Effects of a protein –free diet or food restriction on the immune system of Wistar and Buffalo rats at different ages. Mechanism of Ageing and Development, 72, 183-197.

12. Meghelli-Bouchenak M, Boguillon M, Belleville J (1987): Time course of changes in rat serum apolipoproteins during the consumption of different low protein diets followed by a balanced diet. J. Nutr., 117, 641-649.

13. Ogunkeye O O, Ighogboja I S (1992): Increase in total serum triglyceride and phospholipids in kwashiorkor. Ann. Trop. Pediatr., 12, 463-466.

14. Santana D M G, Molinan S L, Miranda- Neto M H (2001): Effects of protein and vitamin B deficiency on blood parameters and myenteric neurons of colon of rats. Arg. Neuropsiquiatr., 59, 3-A, 493-498.

15. SPSS. (1999): SPSS 10.0 for windows. SPSS Inc., Chicago, IL.

16. Rafael J F, Mora M D (1999): Malnutrition; Organic and functional consequences. World J. Surg., 23, 530-535.

17. Rana S, Sodhi C P, Mehta S, Vaiphei K, Katyal R, Thakur S, Mehta S K (1996): Protein- energy malnutrition and oxidative injury in growing rats. Human and Exp. Toxic., 15, 810-814.

18. Udani P M (1992): Protein energy malnutrition, brain and various facets of child development. Indian J. Pediatr., 59, 165-186, 1992.

19. Walfrod R L, Mock D, Verdery R, Mccallum T (2002): Calorie restriction in biosphere 2: alterations in physiologic, hematological, hormonal and biochemical parameters in humans restricted for a 2. year period. J. Gerontol. A Biol. Sci. Med. Sci., 57(6), B211- 224.
Yüzüncü Yıl Üniversitesi Veteriner Fakültesi Dergisi-Cover
  • ISSN: 1017-8422
  • Yayın Aralığı: Yılda 3 Sayı
  • Başlangıç: 1990
  • Yayıncı: Yüzüncü Yıl Üniv. Veteriner Fak.