Nuh KILIÇ, Julia HENKE

Comparative studies on effect of S(+)-Ketamine-Medetomidine and racemic Ketamine- Medetomidine in Mouse

Bu çalısmada, farelerde anestezi için S(+)-ketamin-medetomidin (S-K/MED) kombinasyonu ile ketaminrazemat-medetomidin (K-R/MED) kombinasyonu klinik ve kardiyopulmoner etkileri yönünden karsılastırıldı. Toplam 20 eriskin disi fare iki farklı gruba ayrıldı. Grup 1 (n=10) 0.25 mg/kg MED ve 75 mg/kg S-K intraperitonal yolla uygulanırken, Grup 2 (n=10)’deki farelere yine aynı yolla 0.25 mg/kg MED ve 50 mg/kg K-R verildi. Anestezik ajanların etkileri; indüksiyon, analjezi, immobilizasyon süreleri, vücut ısısı, kalp frekansı, solunum frekansı ve perifer oksijen saturasyonu gibi klinik gözlemlere göre degerlendirildi. Bu gözlemler anesteziden on dakika önce ve anestezi boyunca onar dakikalık aralıklarla yapıldı. Analjezi süresi G1’de ortalama 25 dakika iken (15-35), G2’de ortalama 40 dakika (30-60) olarak ölçüldü (p

Anahtar Kelimeler:

anestetikler, fareler, ketamin, karşılaştırmalar, ağrı kesiciler, medetomidin

Farelerde S(+)-Ketamin-Medetomidin ve Ketamin Razemat-Medetomidin 'in etkilerinin karşılaştırılması

In this study, the cardiopulmonary and clinical effects of S (+) ketamine/medetomidine (S-K/MED) and ketaminrazemat/medetomidine (K-R/MED) combination on mice for anaesthesia were compared. A total of 20 adult female NMRImice (body weight 33-58 g) were used for this trial. Mice were divided into two groups. 75 mg/kg S-K and 0.25 mg/kg MED intraperitoneally (IP) were given to the Group 1 (G1), while 50 mg/kg K-R and 0.25 mg/kg MED intraperitoneally were given to the Group 2 (G2). The efficiency of anaesthetic agents were determined by clinical observations, which duration of induction, analgesia and immobilization, body temperature, respiratory rate (RR), peripheral oxygen saturation (SpO2 ), heart rate (HR). These parameters were measured and recorded at 10-minute intervals before and after administration of the anaesthetic agents. Analgesia lasted as a mean of 25 (15-35) min in G1 and 40 (30-60) min in G2 (p<0.05). The immobilization periods conducted in G1 and G2 were 56 (ranged between 50 and 65) min and 87 (ranged between 75 and 100) min, respectively (p<0.05). Temperatures reduced in both groups during anaesthesia. No significant differences between individual groups in the heart or respiratory rate as well as SpO2 value. Atipamezole (AT) application resulted in an increase in respiratory rate, and a small numbers of animals showed hyperventilation. As a results, it could be suggested that using S-K in combination with MED causes shorter duration time and less side effects after antagonisation of MED compared with K-R.

Keywords:

anaesthetics, mice, ketamine, comparisons, analgesics, medetomidine,

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1.Erhardt W, Wohlrab S, Kılıç N, Henke J (2001): Comparison of the anaesthesia combinations racemic-ketamine/medetomidine and S-Ketaminemedetomidine in Syrian golden hamsters (mesocricetus auratus). J. Vet. Anaest. 28 (4): 212-213.

2.Flecknell PA (1997): Laboratory animal anaesthesia. (2nd Ed.). Academic Press, London.

3.Kılıç N, Henke J, Pragst I, Echardt W (2001): Comparison of three different medetomidin/ketamin combinations in NMRI mice. J. Vet. Anaest. 28 (4): 211-212.

4.Macdonald E, Scheinin M, Scheinin H (1988): Behavioural and neurochemical effects of medetomidine, a novel veterinary sedative. Eur. J. Pharmacol. 158: 119-127.

5.Morris TH (1991): Use of medetomidine and atipemazole in laboratory animals. Proceedings of the 4th Internatinal Congress of Veterinary Anaesthesia, Utrecht, Special supplement of the J. Vet. Anaesth. 277-279.

6.Nevalainen T, Pyhala L, Voipio HM, Virtanen R (1989): Evaluation of anaesthetic potency of medetomidine-ketamine combination in rats, guinea-pigs and rabbits. Acta Vet. Scandinavica. 85: 139-143.

7.Pfenninger E, Baier C, Claus S (1992): Analgesia and psychic side-effects under ketamine racemate versus S-(+)-ketamine in analgesic doses. Anesthesiology. 77: 413-415.

8.Savola JM (1989): Cardiovascular actions of medetomidine and their reversal by atipemazole. Acta. Vet. Scand. 85: 39-47.

9.Stelter A (2001): Die Anästhesie bei der Katze mit Medetomidin und Ketamin bzw. S-Ketamin - eine klinische Studie.Vet. Medical. Diss., München.

10.Tunkel F (2001): Die Anästhesie beim Hund mit Ketamin-Razemat/Medetomidin im Vergleich zu S-(+)-Ketamin/Medetomidin; Eine klinische Studie. Vet. Medical. Diss., München.

11.Vaehe-Vaehe T (1989): Clinical evaluation of medetomidine, a novel sedative and analgesic drug for dogs and cats. Acta Vet Scand. 30: 267-273.

12.Vainio O (1989): Introduction to the clinical pharmacology of medetomidine. Act. Vet. Scan. Supp. 85: 85-88.

13.Virtanen J, Savola JM, Saano V, Nyman L (1988): Characterization of the selectivity, specifity and potency of medetomidine as an a2-adrenoceptor agonist. Act. Vet. Scand. 85: 29-37.

14.Voipio HM, Nevalainen TO, Virtanen R (1990): Evaluation of anesthetic potency of medetomidine-ketamine combination in mice. IX ICLAS Symposium Proceedings. pp. 298-299.

15.Wijngergen A (1995): Krankheiten der Heimtiere, 3.Auflage, Schlütersche, Hannover.

16.Wixson S (1994): Anaesthesia and analgesia. In, Smith AC, Swindle MM (Eds): Rabbits and Rodents. The Scientists Center for Animal Welfare, Williams & Wilkins, Baltimore, 59-75.