Sığır Fekal Örneklerinden Salmonella spp. ve Escherichia coli O157:H7’ye Özgü Bakteriyofaj Varlığının Araştırılması

Salmonella Typhimurium (S. Typhimurium) ve Escherichia coli (E. coli) O157:H7, virülanslarına, antimikrobiyal dirençlerine ve yüzeylerde hayatta kalmalarına katkıda bulunan bakteriyel biyofilmler oluşturan ve dünya çapında ciddi gıda zehirlenmelerine neden olan önemli gıda kaynaklı patojenlerdir. Bakteriyofajlar, gıda kaynaklı patojenleri kontrol etmek adına her geçen gün artarak kullanılan antibakteriyel maddeler olup ayrıca antibiyotik direncin gelişmesine karşı çözümde rol oynamaktadır. Ayrıca bakteriyofajlar atık sularda, doğada ve hayvansal atıklarda olabileceği gibi birçok gıda maddesinde bulunmaktadır. Bu çalışmanın amacı sığır altlıklarından alınan dışkı örnekleri kullanılarak ülkemizde ve dünyada sık rastlanan Salmonella türleri. ve E. coli infeksiyonları ile mücadele etmede etkili olabilecek, ülkemizde sirküle olan Salmonella spp. ve E. coli spesifik spot test ile fajların purifikasyonu ve litik etkilerinin belirlenmesi amaçlanmıştır. Bu çalışmada 3 tane S. Typhimurium ve 1 tane E. coli O157:H7 bakteriyofajları izole edilerek, litik aktiviteleri belirlenmiştir. Sonuç olarak bu çalışmada Aksaray ilinden pürifiye edilen Salmonella Typhimurium ve E. coli O157:H7 bakteriyofajlarının tespit edilen litik aktiviteleri ile Salmonella Typhimurium ve E. coli O157:H7 kaynaklı enfeksiyonların tedavisi ve gıda endüstrisinde korunma çalışmalarına ışık tutabileceği düşünülmektedir.

Anahtar Kelimeler:

Bakteriyofaj, E. coli O157:H7, Fekal, Pürifikasyon, S. Typhimurium.

Investigation of Escherichia coli O157:H7 and Salmonella Bacteriophages in Cattle Fecal Sources

S. Typhimurium and E. coli O157:H7 are the most important foodborne pathogens forming bacterial biofilms that contribute to their virulence, antimicrobial resistance, and surface survival, causing severe food poisoning outbreaks worldwide. Bacteriophages are antibacterial agents that are increasingly used to control foodborne pathogens, and they also play a role in the solution against the development of antibiotic resistance. In addition, bacteriophages can be found in wastewater, natural and animal wastes, and foodstuffs. Aim of this study to determine the purification and lytic effects of Salmonella spp. and E. coli specific phages circulating in our country, which can effectively combat common Salmonella spp. and E. coli infections in our country and the world by using samples taken from the cowshed. In this study, 3 S. Typhimurium and 1 E. coli O157:H5 bacteriophages were isolated, and their lytic activities were determined. As a result, it is thought that the lytic activities of S. Typhimurium and E. coli O157:H7 bacteriophages purified from Aksaray province in this study can shed light on the treatment of S. Typhimurium and E. coli O157:H7 infections and prevention studies in the food industry.

Keywords:

Bacteriophage, E. coli O157:H7, Fecal, Purification, S.Typhimurium,

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