Farklı Doz Ellajik Asitin Rat Serum Lipit Profili ve Total Antioksidan Üzerine Etkisi

Bu çalışma, ellajik asitin, lipit, lipoprotein ve total antioksidan kapasite üzerine optimum etkiyi hangi dozda gösterdiğini tespit etmek amacıyla planlandı. Bu amaçla 40 adet, 200-250 gr ağırlığında erkek rat (Wistar- albino) kullanıldı. Kontrol, 1. grup (EA 3 mg/kg), 2. grup (EA 10 mg/kg), 3. grup (EA 12 mg/kg) ve 4. grup (EA 20 mg/kg) olmak üzere her biri 8 rattan oluşan 5 grup oluşturuldu. Sekiz hafta boyunca, gün aşırı olarak ellajik asitin (EA) oral olarak uygulanmasını takiben, alınan kan örneklerinde, trigliserit, kolesterol, HDL ve LDL ölçümleri ticari kit kullanılarak modüler oto-anlizör cihazında yapıldı. VLDL düzeyleri formülle hesaplandı. TAS ölçümü ticari kit kullanılarak spektrofotometrede gerçekleştirildi. Kontrol grubu ile karşılaştırıldığında, EA verilen tüm gruplarda, TAS, HDL, VLDL ve trigliserit seviyelerinin artarken, LDL ve kolesterol seviyelerinin azaldığı tespit edildi. Bu değişiklikler istatistik olarak anlamlı değildi (p˃0.05). Sonuç olarak, bu çalışmada elde edilen verilere dayanarak, yapılacak çalışmalarda, ellajik asitin koruyucu dozunun 20 mg/kg/gün düzeyinden yüksek olması ve uzun süre kullanılması önerilir

Anahtar Kelimeler:

Ellajik asit, Rat, HDL, LDL, Trigliserid, Kolesterol, TAS

The Effect of Different Doses of Ellagic Acid on the Serum Lipid Profile and Total Antioxidant of Rat

This study was planned to determine in which dose of ellagic acid it shows the optimum effect on lipid, lipoprotein and total antioxidant capacity. For this purpose, 40 male rats (Wistar-albino) weighing 200- 250 g were used. Control, group 1 (EA 3 mg/kg), group 2 (EA 10 mg/kg), group 3 (EA 12 mg/kg) and group 4 (EA 20 mg /kg) were designated, each containing 8 rats. Following orally application ofellagic acid (EA) every other day for eight weeks, from the blood samples taken; the measurement of triglyceride, cholesterol, LDL and HDL were determined by modular auto-analyzer using commercial kit. VLDL levels were calculated by formula. The total antioxidant capacity (TAS) was measured through spectrophotometer using the commercial kit. When compared with the control group, it was found that while TAS, triglyceride, HDL and VLDL levels were increased, LDL and cholesterol levels were decreased in the all EA applied groups. These changes weren’t important statistically (P˃0.05). Consequently, with regard to the data acquired in this study, it is recommended that in further research, the protective dose of ellagic acid is higher than 20 mg/kg/day and prolonged use

Keywords:

Ellagic acid, Rat, HDL, LDL, Triglyceride, Cholesterol, TAS,

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