Çalkalayıcı Kullanımının Süs Biberi Shed-Mikrospor Kültürlerinde Embriyo Verimine Etkisi

Morfolojik çeşitlilikleri ve çeşitli zengin içerikleri nedeniyle süs biberleri (Capsicum annuum L.) son yıllarda süs bitkisi, gıda, kozmetik ve ilaç sektörlerinde tercih edilen bitkiler haline gelmiştir. Süs biberine olan bu ilgi süs biberinde yapılan ıslah çalışmalarını son yıllarda arttırmıştır. Ancak süs biberlerinde haploid teknolojisine dayalı sınırlı sayıda ıslah çalışması bulunmaktadır. Bu çalışmada, süs biberi shed-mikrospor kültürlerinde çalkalayıcı kullanımının embriyo verimi üzerine etkisi belirlenmeye çalışılmıştır. Toplam 29 süs biberi genotipi kullanılmış ve her genotipin çalkalanan ve durağan kültürleri karşılaştırılmıştır. Çalkalanan kültürler, toplam ve globular embriyo oluşturma açısından daha başarılı androjenik performans göstermiştir. Çalışmada en başarılı embriyojenik performans, hem durağan hem çalkalanan kültürlerde Genotip 754'ten elde edilmiştir. Genotip 754'ün çalkalanan kültürleri, durağan kontrol grubuna kıyasla 4.5 kat daha fazla embriyo oluşturmuştur. Sonuç olarak, çalkalayıcı kullanımı, genotipe bağlı olarak süs biberi shed-mikrospor kültürlerinde embriyo verimini olumlu yönde etkilemiştir.

Anahtar Kelimeler:

Anter, Capsicum annuum L., doubled haploid, mikrospor embriyogenesis, çalkalama

The Effect of Shaker Use on Embryo Yield in Shed-Microspore Cultures of Ornamental Peppers

Because of their morphological diversity and various rich content, ornamental peppers (Capsicum annuum L.) has become a favored plant in ornamental plant, food, cosmetics and medicinal sectors in recent years. This interest increased the ornamental pepper breeding studies. However, there are limited numbers of study based on haploid technology in ornamental peppers. In this study, we aimed to determine the effect of using shaker on embryo yield in the shed-microspore cultures of ornamental peppers. We used a total of 29 genotypes and compared the shaken and stationary cultures for each genotype. The shaken cultures gave more successful androgenic performance in terms of total and globular embryo formations. The most successful embryogenic performance was obtained from Genotype 754 in both stationary and shaken cultures. However, the shaken cultures of Genotype 754 formed 4.5 times more embryos when compared to its stationary control group. As the result, the use of shaker positively affected embryo yield in shed-microspore cultures of ornamental pepper depending on genotype.

Keywords:

Capsicum annuum L., doubled haploid, microspore embryogenesis, culture shaking,

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