The effect of saccharomyces cerevisiae on in vitro rumen digestibilities of dry matter, organic matter and neutral detergent fibre of different forage: Concenrate ratios in diets

Bu araştırmanın amacı farklı kaba: konsantre yem oranına sahip rasyonlara Saccharomyces cerevisiae canlı maya kültürü ilavesinin (Yea-Sacc1026,5x109 organisms/g) in vitro rumen kuru madde, organik madde ve neutral detergent fibre sindirilebilirliği üzerine etkisini incelemektir. Araştırmada rumen kanülü takılmış 4 baş koyun, 4x4 latin kare düzeninde kullanılmış ve sırasıyla %70 yonca kuru otu + %30 konsantre yem, %70 yonca kuru otu + %30 konsantre yem + 4 g Yea-Sacc1026 ,%30 yonca kuru otu + %70 konsantre yem + 4g Yea-Sacc1026 ve %30 yonca kuru otu + % 70 konsantre yemden oluşan rasyonlarla beslenmişlerdir. Araştırmanın her bir periyodu 20 gün sürmüştür. Yirminci günde, koyunlardan in vitro inkübasyon için sabah yemlemesinden 2 saat sonra rumen sıvıları toplanmıştır. Denemenin sonunda kaba yem ağırlıklı rasyona maya kültürü ilave edilmesi in vitro rumen kuru madde, organik madde ve neutral detergent fibre sindirilebilirliklerini önemli derecede artırırken (P

Anahtar Kelimeler:

Saccharomyces cerevisiae, kuru madde, organik madde, bitkisel yem, sindirilebilirlik, rumen sindirimi, lif, diyetler, in vitro sindirilebilirlik

Saccharomyces cerevisiae' nin kaba: Konsantre yem oranı farklı rasyonlarda in vitro rumen kuru madde, organik madde ve neutral detergent fibe sindirilebilirliği üzerine etkisi

The aim of this research was to investigate the effect of supplementation of Saccharomyces cerevisiae viable yeast culture (Yea-Sacc1026,5x109 organisms/g) into diets having different forage: concentrate ratio on the in vitro rumen dry matter, organic matter and neutral detergent fibre digestibilities. In this research, four cannulated sheep were used in 4x4 latin square design and fed on 70 % alfalfa hay + 30 % concentrate (Diet I), 70 % alfalfa hay + 30 % concentrate + 4 g Yea-Sacc1026 (Diet II), 30 % alfalfa hay + 70 % concentrate + 4 g Yea-Sacc1026 (Diet III) and 30 % alfalfa hay + 70 % concentrate (Diet IV) respectively. The duration of each experimental period was 20 days. On the 20th day, rumen digesta was collected from sheep for in vitro incubation 2 h after morning feeding. At the end of experiment, supplementation of yeast culture on high forage ration significantly increased the in vitro rumen dry matter, organic matter and neutral detergent fibre digestibilities (P<0,001), while did not affect on high concentrate ration.

Keywords:

Saccharomyces cerevisiae, dry matter, organic matter, forage, digestibility, rumen digestion, fibre, diets, in vitro digestibility,

PDF

___

1. AOAC: Official Methods of Analysis, 14 th Ed. Assos. Of. Agr. Chem. Washington DC, 1984
2. AREMBEL, MJ.; KENT, B.A.: Effect of yeast culture on nutrient digestibility and milk yield re sponse in early to midlactation dairy cows. J. Dairy Sci. 73:1560-1563, 1990.
3. CARRO, M.D.; LEBZIEN, P.; ROHR, K.: Effects of yeast culture on rumen fermentation, digestibil ity and duodenal flow in dairy cows fed a silage based diet. Livestock Production Science, 32:219- 229,1992.
4. CHADEMANA, I.; OFFER, N.W.: The effect of dietary inclusion of yeast culture on digestion in the sheep. Anim. Prod. 50:483-489, 1990.
5. CHAUCHEYRAS, F.; FONTY, G.; BERTIN, G.; GOUETH, P.H.: Effects of Saccharomyces cere visiae on ruminal microbial activities in vitro. Ab stracts of Satellite Symposium of VII ISRP, Ma nipulation of Ruminal Digestion, 2-5, Oct. Starele sna, Slovakia, 1994, 29.
6. CHIQUETTE, J.: Saccharomyces cerevisiae and Aspergillus oryzae, used alone or in combination, as a feed supplement for beef and dairy cattle. Ca nadian J. Anim. Sci. 75: 405-415, 1995.
7. DAWSON, K.A.: Designing the yeast culture of tomorrow-mode of action of yeast culture for ru minants and non-ruminants. Ed. Lyons, T.P., Bio technology in the Feed Industry, Vol. VII, Alltech Technical Pub. Nicholasville, KY., 59-77, 1990.
8. DAWSON, K.A.; NEWMAN, K.E.; BOLING, J.A.: Effects of microbial supplements containing yeast and lactobacilli on roughage-fed ruminal mi crobial activities. J. Anim. Sci. 68: 3392-3398, 1990.
9. DAWSON, K.A.; HOPKINS, D.M.: Differential effects live yeast on the celuloytic activities of an aerobic ruminal bacteria. J. Anim. Sci. 69 (Suppl.I): 531, 1991
10. DÜZGÜNES, O.; KESİCİ, T.; KAVUNCU, O.; GÜRBÜZ, F.: Araştırma ve Deneme Metodları (Istatistik Metodları-II). In Press of University of Ankara (No:1021-295)1987 Ankara
11. FULLER, R.: Probiotic in man and animals. Jour nal of Applied Bacteriology, 66:365-368, 1989.
12. HARRISON, G.A.; HEMKEN, R.W.; DAWSON, K.A.; HARMON, R.J.; BARKER, K.B.: Influence of addition of yeast culture supplement to diets of lactating cows on ruminal fermentation and micro bial populations. J. Dairy Sci. 71:2967-2975, 1988.
13. HUGHES, J.: The effect of a high-strength yeast culture in the diet of early weaned calves. Anim. Production, 46:526, 1988.
14. KIMENAI, R.: Yea-Sacc 1026 effects on bull beef performance and carcass characteristics in a low concentrate input program. Ed. Lyons, T.P., Bio technology in the Feed Industry, Vol. VII, Alltech Technical Pub. Nicholasville, KY., 496, 1990.
15. MARTIN, S.A.; NISBET, D.J.: Effect of direct fed microbials on rumen microbial fermentation. J. Dairy Sci. 75: 1736-1744, 1992.
16. McLEOD, K.R.; KARR, K.J.; DAWSON, K.A.; AORON, D.K.; MITCHELL, G.E.: Influence of yeast culture and monensin on ruminal metabolic end products and feedlot cattle performance. J. Anim. Sci., 69 (Suppl.I): 158, 1991.
17. MICHAEL, D.: Effect of Yea-Sacc 1026 on feed intake and performance of finishing bulls. Ed. Ly ons, T.P., Biotechnology in the Feed Industry, Vol. VII, Alltech Technical Pub. Nicholasville, KY., 495, 1990.
18. MUTSVANGWA, T.; EDWARDS, I.E.; TOPPS, J.H.; PATERSON, G.F.M.: The effect of dietary inclusion of yeast culture (Yea-Sacc) on patterns of rumen fermentation, food intake and growth of in tensively fed. Anim. Prod. 55:35-40, 1992.