Berrin ZIK, Hakan BİRİCİK, Nesrin ÖZFİLİZ, Korhan ALTUNBAŞ

Farklı karma yem fomları ile beslenen karayaka kuzuları Rumenlerinin histolojik ve morfometrik yönden incelenmesi

: Bu çalışmada, farklı karma yem formlarının rumen mukozası üzerine etkisinin histolojik yönden incelenmesi amaçlandı. Araştırma, her biri 5'er kuzudan oluşan 3 grup üzerinde yürütülmüştür. Kuzular, deneme boyunca sırasıyla toz karma yem + yonca kuru otu, öğütülmemiş karma yem + yonca kuru otu ve sadece öğütülmemiş karma yem ile beslenmişlerdir. Araştırmada deneme sonu hayvanların rumenlerinin atrium, kaudodorsal, dorsal ve ventral bölgelerinden alınan örneklerde histolojik ve morfometrik değerlendirmeler yapıldı. Sonuç olarak I. grup beslemeden III. grup beslemeye doğru rumen bölgelerinde keratinizasyonun ve PAS pozitif reaksiyonun arttığı ve papillaların dallanmaya başladığı, veziküler hücrelerin ise azaldığı gözlendi. Ayrıca morfometrik ölçümlerde I. grup beslemeden III. grup beslemeye doğru papilla genişliği ve uzunluğunun azaldığı belirlendi.

Anahtar Kelimeler:

kuzu, rumen, doku bilimi, morfometri, koyun, Karayaka, karma yemler, beslenme

The histological and morphometrical investigation of the rumens of Karayaka lambs fed with different compound feed forms

The objective of this study was to determined histological effects of different forms of compound feed on rumen mucosa.This experiment was conducted on three groups, each containing five lambs. Lambs were during the fattening period with ground compound feed+alfalfa hay, ungrounded compound feed+alfalfa hay and only ungrounded compound feed, respectively. Histological samples were taken at the end of the experiment from the atrium, caudodorsal, dorsal and ventral regions of the rumens of animals and were investigated histologically and morphometrically.As a result of the study an increase in keratinization, PAS-positivity and branch formation in papillae were observed from I. group feeding to III.group feeding, respectively. The decrease in number of vesicular cells were also seen. Additionally it was determined that papilla width and length decreased from I. group feeding to III. group feeding.

Keywords:

lambs, rumen, histology, morphometrics, sheep, Karayaka, compound feeds, nutrition,

PDF

___

1. Bölükbaşı, F.: Midede sindirim, Fizyoloji Ders Kitabı. Ankara, Ankara Üniversitesi Basımevi. 132-226; 1989.
2. Josefsen, T.D., Aagnes, T.H., Mathiesen, S.D.: Influence of Diet on the Morphology of the Ruminal Papillae in Reindeer Calves. Rangifer. 1996; 16, (3): 119-28.
3. Lane, M.A., Baldwin V., Jesse B.W.: Sheep Ru men Metabolic Development in Response to Age and Dietary Treatments. Journal of Animal Science. 2000;78,(7):1990-96.
4. Goodlad, R.A.: Some Effects of Diet on the Mitotic Index and the Cell Cycle of the Ruminal Epithelium of Sheep. Q. J. Exp. Physiol. 1981; 66, (4): 487-99.
5. Josefsen, T.D., Landsverk, T.: T cell Subsets and Langerhans Cells in the Forestomach Mucosa of Adult Sheep and Sheep Foetuses. Vet. Immunol. Immunopathol.1996; 51, (1-2): 101- 11.
6. Baran, M., Marcanik, J., Kocisova, J., Zelenak I., Belak M.: The Relationship Between Fermentation and the Morphological Structure of Rumen Epithelium in Sheep Fed Pelleted Feeds. Vet. Med. (Praha). 1979; 24, (10):587-95.
7. Zitnan, R., Bomba, A., Laukova, A., Sommer, A., Kolodzieyski, L., Venglovsky, J., Bindas, L.: The Effect of Diet Composition on the Development of Rumen Digestion in Lambs. Arch. Tierernahr. 1993; 45, (2): 161-71.
8. Arias, J.L., Vial, E., Cabrera, R.: Observations on the Histogenesis of Bovine Ruminal Papillae. Am. J. Vet. Res. 1980;41,(2):174-8.
9. Franco, A., Regodon, S., Robina A., Redondo E.: Histomorphometric Analysis of the Rumen of Sheep During Development. Am. J. Vet. Res. 1992; 53,(7):1209-17.
10. Swan, G.E., Groenewald, H.B.: Morphological Changes Associated with the Development of the Rumino-Reticulum in Growing Lambs Fed Different Rations. Onderstepoort J. Vet. Res. 2000;67,(2):105-14.
11. Zitnan, R., Voigt, J., Wegner, J., Breves, G., Schroder, B., Winckler, C., Levkut, M., Kokardova, M., Schonhusen, U., Kuhla S., Hagemeister, H., Sommer A.: Morphological and Functional Development of the Rumen in the Calf: Influence of the Time of Weaning. 1. Morphological Development of Rumen Mucosa. Arch Tierernahr. 1999; 52, (4): 351-62.
12. Zitnan, R., Voigt, J., Schonhusen, U., Wegner, J., Kokardova, M., Hagemeister, H., Levkut, M., Kuhla, S., Sommer, A.: Influence of Dietary Concentrate to Forage Ratio on the Development of Rumen Mucosa in Calves. Arch. Tierernahr. 1998;51,(4): 279-91.
13. Weiss, B.: The Relationship between the Acidity of the Rumen Contents and the Proliferation Status of the Rumen Mucosa in Cattle. Berl. Munch. Tierarztl. Wochenschr. 1994; 107, (3): 73-8.
14. McGavin, M.D., Morrill, J.L.: Scanning Electron Microscopy of Ruminal Papillae in Calves Fed Various Amounts and Forms of Roughage. Am. J. Vet. Res. 1976; 37, (5): 497-508.
15. Szarek, J.,Iwanska, S., Bomba, G., Pysera, B., Strusinska, D.: Morphological Structure of the Mucous Membrane and Submucosa of Rumen in Calves Receiving Synthetic or Natural β-Carotene and Vitamins AD3E. 1992; 40, (4):303-9.
16. Zitnan, R., Bomba, A., Sommer, A., Kolodzieyski, L.: Development of Rumen Metabolism and Ruminal Epithelium in Lambs. Arch. Tierernahr. 1993; 44, (3): 227-33.
17. Josefsen, T.D., Aagnes T.H., Mathiesen, D.: Influence of Diet on the Occurrence of Intraepithelial Microabscesses and Foreign Bodies in the Ruminal Mucosa of Reindeer Calves. Jornal of Veterinary Medicine Series A. 1997; 44, (5): 249-257.
18. Yamamoto, Y., Atoji, Y., Agungpriyono, S., Suzu ki, Y.: Morphological Study of the Forestomach of the Japanese Serow (Capricornis crispus). Anat. Histol. Embryol. 1998; 27, (2):73-81.
19. Hinterdorfer, F., Deutz A.: Hyperplasia of the Ruminal Villi in Three Wild Roe Deer. Tierarztl Prax. 1996; 24, (4):357-358.
20. Groenewald, H.B.: Ultrastructure of the Epithelium of the Rumen, Reticulum and Omasum of Grey, White and Black Karakul Lambs. Onderstepoort J. Vet. Res. 1993; 60, (3): 197-204.
21. Singh, M., Nagpal, S.K., Singh Y.: Histological Characterization of Nonglandular Mucosa of Ru- men in Camel (Camelus dromedarius). Journal of Animal Sciences. 1993; 63, (12): 12641266.
22. Türkmen, I.I., Balcı, F.: Farklı Karma Yem Form larının Karayaka Kuzuların Besi Performansı ile Kesim ve Karkas Özellikleri Üzerine Etkisi. U.Ü. Veteriner Fakültesi Dergisi. 1999; 18, (1-2): 19-28.
23. National Academy of Science (NRC).: Nutrient Requirement of Sheep. Washington, D. C., 45-48, 1985.
24. Böck, P.: Romeis Mikroskopische Technik., München, 17. Aufl. Urban und Schwarzenberg., 561, 1989.
25. Mc Manus, J.F. A.: Mc Manus Method for Glycogen, Stain Tech., 23: 99-108, 1948.
26. Ayoup, P., Shklar, G.: J. Oral Surg. Coyright by American Dental Assciation, 1963; 16, 580-1.
27. Sümbüloğlu, K., Sümbüloğlu, V.: Biyoistatistik, Ankara, Özdemir Yayıncılık, 152, 1994.
28. Greenwood, R.H., Morrill, J.L., Titgemeyer, E.C., Kennedy, G.A.: A New Method of Measuring Diet Abrasion and its Effect on the Development of the Forestomach. J. Dairy Sci. 1997; 80, 2534-41.