Al Mukhlas FİKRİ, Ahmad SULAEMAN, Sri Anna MARLİYATİ, Mokhamad FAHRUDİN, Ekowati HANDHARYANİ

Endonezya Propolis'in Hamile Farelerde İmmünomodülatör Etkisi: Bir Ön Sonuç

Propolisin immünomodülatör aktiviteye sahip olduğu yaygın olarak kabul edilmiştir. Bununla birlikte, hamilelik sırasındaki aktivite henüz araştırılmamıştır. Bu çalışma, hamilelik sırasında propolis uygulamasının anne fare dalağının histolojik değişiklikleri üzerindeki etkisini analiz etmeyi amaçladı. Toplam 25 hamile fare, Güney Sulawesi propolisinin kontrol (%1 Tween 80), düşük (380 mg/kg) ve yüksek doz (1400 mg/kg) etanol özütü ve düşük (380 mg/kg) ve yüksek dozlu (1400 mg/kg) Banten propolisinin su özütü olmak üzere 5 gruba ayrıldı. Propolis uygulaması gebelik boyunca günlük olarak yapıldı. Gebeliğin 18. gününde fareler, hematoksilen ve eozin boyaması kullanılarak histolojik değerlendirme için kullanılan dalak elde etmek için sakrifiye edildi. Beyaz pulp sayısı ve çapı 10x büyütmeli mikroskop altında gözlendi. Sonuçlar, düşük dozdaki tüm propolis ekstraktlarının beyaz pulp sayısını önemli ölçüde arttırdığını gösterdi (p < 0.05). Bununla birlikte, propolis uygulanan tüm gruplarda çaptaki bir artış anlamlı bulunmadı. Bu çalışma, Endonezya propolisinin annenin bağışıklık sistemini modüle edebileceğini düşündürmektedir.

Anahtar Kelimeler:

Immunomodulatori, histoloji, hamilelik

IMMUNOMODULTATORY EFFECT OF INDONESIAN PROPOLIS IN PREGNANT MICE: A PRELIMINARY RESULT

Propolis has been widely accepted to exhibit immunomodulatory activity. However, the activity during pregnancy has not been investigated yet. This study was a preliminary study which aimed to analyze the effect of several types of Indonesian propolis on the histological changes of maternal mice spleen. A total of 25 pregnant mice were divided into 5 groups, control (1% Tween 80) group, low (380 mg/kg) and high-dose (1400 mg/kg) ethanol extract of propolis from South Sulawesi groups, and low (380 mg/kg) and high-dose (1400 mg/kg) water extract of propolis from Banten groups. Propolis samples were administered daily during pregnancy. At day 18 of gestation, the mice were sacrificed to obtain spleen which was used for histological evaluation using hematoxylin and eosin staining. The number and diameter of white pulp were observed under the 10x magnificent of microscope. The results showed that all propolis extracts at low-dose significantly increased the number of white pulp (p < 0.05). However, an increase in the diameter was found not significant in all propolis-administered groups. This study suggests that Indonesian propolis may modulate maternal immune system.

Keywords:

immunomodulator histology, pregnancy, propolis,

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