Zehra CAN, Yakup KARA, Sevgi KOLAYLI, İbrahim ÇAKMAK

Türkiye'nin Marmara Bölgesinden Elde Edilen Bazı Propolislerin Anti-üreaz Aktivitesinin Belirlenmesi

Propolis, bal arıları tarafından bitkilerden özellikle çiçek ve tomurcuklardan balmumu ve reçine ile karıştırılarak toplanan ve kovanda birçok amaç için kullanılan doğal bir üründür. Propolisin uzun yıllardır geleneksel tıpta çeşitli hastalıkların tedavisinde kullanıldığı ve antioksidan, antimikrobiyal, antiülser, antitümör, antiinflamatuar gibi biyolojik aktiviteleri olduğu bilinmektedir. Helicobakter pilori'yi (H. pylori) inhibe etmeye ve böylece birçok mide hastalığını tedavi etmeye yönelik alternatif yaklaşımlara artan bir ilgi vardır. Bu çalışma, Türkiye'nin Marmara bölgesinden alınan ham propolis örneklerinin çeşitli özelliklerini araştırmıştır. Propolis örneklerinde anti-üreaz aktivitesi araştırıldı. IC50 değerlerinin anti-üreaz aktivitesi 1.110 ila 5.870 mg/mL aralığındaydı. Sonuç olarak propolis ekstraktının H. pylori tedavisinde insan sağlığını iyileştirmek için kullanılabilecek iyi bir inhibitör olduğu söylenebilir.

Anahtar Kelimeler:

Propolis, üreaz, enzim inhibisyonu

DETERMINATION OF ANTI-UREASE ACTIVITY OF PROPOLIS FROM MARMARA REGION OF TURKEY

Propolis is a natural product collected by honeybees from plants especially flowers and buds by mixing with wax and resin and used for many purposes in hive. It is known that propolis has been used in the treatment of various diseases in traditional medicine for many years and has biological activities such as antioxidant, antimicrobial, antiulcer, antitumor, anti-inflammatory. There is increasing interest in alternative approaches to inhibit Helicobacter pylori (H. pylori) and thereby treat many gastric diseases. This study investigated various properties of raw propolis samples from the Marmara region of Turkey. Anti-urease activity was investigated in propolis samples. The anti-urease activity of IC50 values ranged from 1.110 to 5.870 mg/mL. As a result, it can be said that propolis extract is a good inhibitor that can be used in the treatment of H. pylori to improve human health.

Keywords:

Propolis, urease, enyzme inhibition,

PDF

___

Referans 1 Alanazi, S., Alenzi, N., Alenazi, F., Tabassum, H., Watson, D. 2021. Chemical characterization of Saudi propolis and its antiparasitic and anticancer properties. Scientific reports, 11(1), 1-9.

Referans 2 Amin, M., Anwar, F., Naz, F., Mehmood, T., Saari, N. 2013. Anti-Helicobacter pylori and urease inhibition activities of some traditional medicinal plants Molecules, 18(2), 2135-2149., doi.org/ 10.3390/molecules18022135.

Referans 3 Baltas, N., Yildiz, O., Kolayli, S. 2016a. Inhibition properties of propolis extracts to some clinically important enzymes J. Enzyme Inhib. Med. Chem. 31(S1): 52-55., doi.org/10.3109/14756366.2016.1167049.

Referans 4 Baltas, N., Karaoglu, S. A., Tarakci, C., Kolayli, S. 2016b. Effect of propolis in gastric disorders: inhibition studies on the growth of Helicobacter pylori and production of its urease J. Enzyme Inhib. Med. Chem. 31(sup2), 46-50., doi.org/10.1080/14756366.2016.1186023.

Referans 5 Bankova, V.S. 2005. Chemical diversity of propolis and the problem of standardization, J. Ethnopharmacol. 100, 114–117., doi.org/ 10.1016/j.jep.2005.05.004.

Referans 6 Can, Z. 2018. Determination of in-vitro antioxidant, anti-urease, anti-hyaluronidase activities by phenolic rich bee products from different region of Turkey. feb-fresenıus Environment bulletin. 27,10, 6858-6866.

Referans 7 Catchpole, O., Mitchell, K., Bloor, S., Davis, P., Suddes, A. 2015. Antiproliferative activity of New Zealand propolis and phenolic compounds vs human colorectal adenocarcinoma cells. Fitoterapia. 106:167–74., doi.org/ 10.1016/j.fitote.2015.09.004.

Referans 8 Dunn, B.E., Cohen, H. Blaser, M.J. 1997. Helicobacter pylori Clinical Microbiol. Reviews, 10, 720–741.

Referans 9 Kolayli, S., Can, Z., Yildiz, O., Sahin, H., Karaoglu, S. A. 2016. A comparative study of the antihyaluronidase, antiurease, antioxidant, antimicrobial and physicochemical properties of different unifloral degrees of chestnut (Castanea sativa Mill.) honeys. J. Enzyme Inhib. Med. Chem., 31(sup3), 96-104. doi. org/10.1080/14756366.2016.1209494.

Referans 10 Manyi-Loh, C.E., Clarke, A.M., Ndip, R.N. 2012. Detection of phytoconsti- tuents in column fractions of n-hexane extract of Goldcrest honey exhibiting anti-Helicobacter pylori activity Arch. Med. Res., 43: 197–204.

Referans 11 Maresca, A., Vullo, D., Scozzafava, A., Supuran, C.T. 2013. Inhibition of the alpha- and beta-carbonic anhydrases from the gastric pathogen Helicobacter pylori with anions. J. Enzyme Inhib. Med. Chem., 2: 388–91. doi.org/10.3109/14756366.2011.649268.

Referans 12 Mégraud, F; Lehn, N., Lind, T., Bayerdorffer, E., O’morain, C., Spiller, R., Unge, P., van Zanten, S.V., Wrangstadh, M., Burman, C.F. 1999. Antimicrobial susceptibility testing of Helicobacter pylori in a large multicenter trial: The MACH 2 study Antimicrob. Agents Chemother., 43, 2747–2752.

Referans 13 Modakh, J.K., Liu, Y.C., Machuca, M.A., Supuran, C.T., Roujeinikova, A. 2015. Structural basis for the inhibition of Helicobacter pylori a-carbonic anhydrase by sulfonamides. PLoS One., 10:e0127149. doi:10.1371/journal.pone. 0127149.

Referans 14 Morishita, S., Nishimori, I., Minakuchi, T., Onishi, S., Takeuchi, H., Sugiura, T., Vullo, D., Scozzafava, A., Supuran, C.T. 2008. Cloning, polymorphism, and inhibition of beta-carbonic anhydrase of Helicobacter pylori J. Gastroenterolog. 43, 849–57., doi.org/10.1007/s00535-008-2240-3.

Referans 15 Romero, M., Freire, J., Pastene, E., García, A., Aranda, M., González, C. 2019. Propolis polyphenolic compounds affect the viability and structure of Helicobacter pylori in vitro Revista Brasileira de Farmacogn. 29, 325-332., doi.org/10.1016/j.bjp.2019.03.002. Referans 16 Sahin, H. 2016. Honey as an apitherapic product: its inhibitory effect on urease and xanthine oxidase J. Enzyme Inhib. Med. Chem. 31: 490–4., doi.org/10.3109/14756366.2015.1039532.

Referans 17 Song, M. Y., Lee, D. Y., Kim, E. H. 2020. Anti-inflammatory and anti-oxidative effect of Korean propolis on Helicobacter pylori-induced gastric damage in vitro J. Microbiol. 58(10), 878-885. Vol. 58, No. 10, pp. 878–885., doi.org/ 10.1007/s12275-020-0277-z.

Referans 18 Stavropoulou, M.I., Stathopoulou, K., Cheilari, A., Benaki, D., Gardikis, K., Chinou, I., Aligiannis, N. 2021. NMR metabolic profiling of Greek propolis samples: Comparative evaluation of their phytochemical compositions and investigation of their anti-ageing and antioxidant properties J. Pharm. Biomed. Anal. 194, 113814., doi.org/ 10.1016/j.jpba.2020.113814.

Referans 19 Ulloa, P. A., Vidal, J., Ávila, M. I., Labbe, M., Cohen, S., Salazar, F. N. 2017. Effect of the addition of propolis extract on bioactive compounds and antioxidant activity of craft beer J. Chem. Article ID 6716053, 7., doi.org/10.1155/2017/6716053.

Referans 20 Weatherburn, M.W. 1967 Phenol-hypochlorite reaction for determination of ammonia Anal. Chem. 39:971–4., doi.org/10.1021/ac60252a045.

Referans 21https://www.alfayapimuhendislik.com/bolge/marmara-bolgesi.html