Çınar SEVERCAN, Suzan MURATOĞLU SEVERCAN, Gülce KOCA, Canan YILMAZ, Özge Tuğçe PAŞAOĞLU, Hatice PAŞAOĞLU

Kurkuminin Fruktoza İndüklenmiş Metabolik Sendromlu Sıçan Modelleri Üzerindeki Etkisinin İncelenmesi

Amaç: Çalışmamızda, fruktoza bağlı metabolik sendrom sıçan modelinde kurkuminin glukoz homeostazı, insülin direnci, inflamasyon ve oksidatif stres üzerindeki olası etkilerinin değerlendirmesini amaçladık. Gereç ve Yöntemler: 24 adet erkek erişkin Wistar albino rat rastgele 4 gruba ayrıldı: kontrol (Grup 1), %20 fruktoz (Grup 2), %20 fruktoz ve 100 mg/kg kurkumin (Grup 3) ve %20 fruktoz, ve 200 mg/kg kurkumin (Grup 4). Serum glukoz, insülin ve plazma lipoprotein seviyeleri otoanalizör tarafından belirlenmiştir. Diğer parametreler ise Elisa Assay Metodu ile incelenmiştir. İki grup arasındaki farkın anlamlılığını belirlemek için Mann-Whitney U testi kullanılarak Bonferroni Düzeltmesi yapılmıştır (p≤0,008). Parametreler arasında ise Spearman Korelasyon Analizi yapılmıştır Bulgular: Grup 2'de Grup 1'e göre artan serum glukozu, yüksek tansiyon, insülin direnci ve dislipidemi gözlenerek Metabolik Sendrom (MetS) başarıyla oluşturulmuştur (p≤0,008). Gruplar arasında serum total antioksidan kapasite (TAC) düzeylerinde farkın anlamlılığı bulunamamıştır. Grup 2'nin serum total oksidan durum (TOS) ve oksidatif stres indeksi (OSI) seviyeleri Grup 1'e göre anlamlı olarak artarken, Grup 3 ve Grup 4'te Grup 2'ye göre bu parametrelerin düzeyleri anlamlı olarak azalmıştır (p≤0,008). Grup 2'nin serum tümör nekrozis faktör-α (TNF-α) düzeyi Grup 1'e göre anlamlı düzeyde artmıştır (p≤0,008). Grup 3 ve Grup 4'ün serum TNF-α düzeyinin Grup 2'ye göre anlamlı düzeyde düştüğü belirlenmiştir (p≤ 0,008). Serum OSI ve TNF-α düzeyleri ile serum HOMA-IR ve TNF-α düzeyleri arasında pozitif korelasyon saptanmıştır (p≤0,01). Sonuç: Bulgularımız, sıçan deneysel hayvan modelinde kurkuminin MetS'e karşı iyileştirici bir etkiye sahip olduğunu göstermiştir

Anahtar Kelimeler:

metabolik sendrom, insülin direnci, oksidatif stres

An Investigation of the Effect of Curcumin on Fructose-Induced Metabolic Syndrome Rat Models

Aim: This study aimed to evaluate the potential effect of curcumin on glucose homeostasis, insulin resistance, inflammation, and oxidative stress in a fructose-induced metabolic syndrome rat model. Material and Methods: 24 male adult Wistar albino rats were randomly separated in to 4 groups: control (Group 1), 20% fructose (Group 2), 20% fructose and 100 mg/kg curcumin (Group 3), and 20% fructose, and 200 mg/kg curcumin (Group 4). Serum glucose, insulin, and plasma lipoprotein levels were determined by an auto-analyzer. Other parameters were determined by the Elisa Assay Method. Mann-Whitney U test was performed using the Bonferroni Correction to determine the significance of the difference between the two group (p≤0.008). Spearman Correlation Analysis was performed among parameters. Results: Metabolic Syndrome (MetS) was successfully created by observing increased serum glucose, high blood pressure, insulin resistance, and dyslipidemia in Group 2 compared to Group 1 (p≤0.008). The significance of serum total antioxidant capacity (TAC) levels among the groups could not be determined. While serum total oxidant status (TOS) and oxidative stress index (OSI) levels of Group 2 increased significantly compared to Group 1, and the levels of these parameters were significantly decreased in Group 3 and Group 4 compared to Group 2 (p≤0.008). The serum tumor necrosis factor-α (TNF-α) level of Group 2 increased significantly compared to Group 1 (p≤0.008). It was determined that the serum TNF-α level of Group 3 and Group 4 decreased significantly compared to Group 2 (p≤0.008). There was a positive correlation between serum OSI and TNF-α levels and serum HOMA-IR and TNF-α levels (p≤0.01). Conclusion: Our findings indicated that curcumin has a healing effect against MetS in the rat experimental animal model.

Keywords:

Metabolic syndrome insulin resistance, oxidative stress, inflammation,

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