Turgay DEPREM, Ebru SARI KARADAĞ, Seyit Ali BİNGÖL, Serap TAŞÇI KORAL, Şahin ASLAN, Serap İLHAN

Histological and histochemical studies on the structure of pancreatic ductsof the goose (Anser anser)

The aim of the present study was to determine the histological structure and histochemical properties of the pancreatic ducts in the goose Anser anser. Pancreatic ducts of 6 male and 6 female geese were examined. Crossman s triple stain and Masson s trichrome stain were used for histological examination, and periodic acid Schiff, alcian blue, periodic acid Schiff/alcian blue, and aldehyde fuchsin/alcian blue stainings were performed for histochemical examination. Dorsal and ventral pancreatic ducts were found that transfer pancreatic secretion into the duodenum of the goose, and these channels were found to have a histologically similar structure. No differences were detected between male and female geese. Duct wall was observed to consist of mucosa, muscle, and adventitial layers. In addition to dark principal, light and basal cells were observed in a simple columnar epithelium layer lining the duct. Glands, including centroacinar cells, were also detected in all layers of duct. Furthermore, lymphoid follicles were observed in the connective tissue of the mucosa. Sulfated and carboxylated mucosubstance-secreting cells were found in the duct as well as neutral, acidic, and mixed mucosubstance-secreting cells. We conclude that the results of our study may contribute to future studies.

PDF

___

1. Sturkie PD. Avian Physiology. 4th ed. New York, NY, USA: Springer Verlag; 1986.
2. Fitzgerald TC. The Coturnix Quail: Anatomy and Histology. Iowa City, IA, USA: Iowa State University Press; 1969.
3. Ku SK, Lee JH, Lee HS. An immunohistochemical study of the insulin-, glucagon- and somatostatin-immunoreactive cells in the developing pancreas of the chicken embryo. Tissue Cell 2000; 32: 5865.
4. Motta MP, Macchiarelli G, Nottola SA, Correr S. Histology of the exocrine pancreas. Microsc Res Techniq 1997; 37: 384398.
5. Gulmez N. Are glands present in goose pancreatic ducts? A light microscope study. J Pancreas 2003; 4: 125128.
6. Gülmez N, Kocamış H, Aslan Ş, Nazlı M. Immunohistochemical distribution of cells containing insulin, glucagon and somatostatin in the goose (Anser anser) pancreas. Turk J Vet Anim Sci 2004; 28: 403407.
7. Mobini B. Histological studies on pancreas of goose (Anser albifrons). Vet Res Forum 2011; 2: 2529.
8. Stornelli MR, Ricciardi MP, Miragliotta V, Coli A, Giannessi E. Morpho-structural study of the pancreas and pancreatic duct in ostrich (Struthio camelus L.) Acta Vet Brno 2006; 75: 157160.
9. Mobini B, Aghaabedi B. Histological and histochemical studies on pancreas of native turkey in Iran. Vet J 2009; 22: 28.
10. Das A, Das RK, Parida S, Mishra UK, Solanki D. Histomorphological study on pancreas of duck (Anas boscas). Indian J Anim Sci 2003; 73: 598599.
11. Mobini B. Histochemical and histological studies on the pancreas in mature pigeon (Columba Livia). Eur J Exp Biol 2013; 3: 148152.
12. Al-Agele RAA, Mohammed FS. Architecture morphology and histological investigations of pancreas in golden eagles (Aquila chrysaetos). Al Anbar J Vet Sci 2012; 5: 149155.
13. Şimşek N, Bayraktaroğlu AG, Altunay H. Localization of insulin immunopositive cells and histochemical structure of the pancreas in falcons (Falco anaumanni). Ankara Üniv Vet Fak Derg 2009; 56: 241247.
14. Şimşek N, Özudoğru Z, Alabay B. Immunohistochemical studies on the splenic lobe of the pancreas in young Japanese quails (Coturnix c. japonica). Deut Tierarztl Woch 2008; 115: 189193.
15. Böck P, Abdel-Moneim M, Egerbacher M. Development of pancreas. Microsc Res Techniq 1997; 37: 374383.
16. Hodges RD. The Histology of the Fowl. New York, NY, USA: Academic Press; 1974.
17. Yoshizawa S. Studies on pancreatic duct system. I) The fine structure of the major pancreatic ducts of normal and chronic pancreas injury dogs. Gastroenterol Jpn 1978; 13: 213223.
18. Madden ME, Sarras MP. The pancreatic ductal system of the rat: cell diversity, ultrastructure, and innervation. Pancreas 1989; 4: 472485.
19. McMinn RMH, Kugler JH. The glands of the bile and pancreatic ducts: autoradiographic and histochemical studies. J Anat 1961; 95: 111.
20. Kendrey G, Roe FJC. Histopathological changes in the pancreas of laboratory rats. Lab Animal 1969; 3: 207220.
21. Kadhim KK, Zuki ABZ, Noordin MM, Babjee SMA, Zamri-Saad M. Morphological study of pancreatic duct in red jungle fowl. Afr J Biotechnol 2010; 9: 72097215.
22. Bancroft JD, Cook HC. Manual of Histological Techniques. Edinburgh, UK: Churchill Livingstone; 1984.
23. Smith PH. Pancreatic islets of the Coturnix quail. A light and electron microscopic study with special reference to the islet organ of the splenic lobe. Anat Rec 1974; 178: 567585.
24. Rawdon BB. Morphogenesis and differentiation of the avian endocrine pancreas, with particular reference to experimental studies on the chick embryo. Microsc Res Techniq 1998; 43: 292305.
25. Egerbacher M, Böck P. Morphology of the pancreatic duct system in mammals. Microsc Res Techniq 1997; 37: 407417.
26. Kodama T. A light and electron microscopic study on the pancreatic ductal system. Acta Pathol Japon 1983; 33: 297321.
27. Böck P, Geleff S. Pancreatic duct glands III. Morphology of secretory epithelium and endoepithelial glands. Z Mikrosk Anat Forsc 1984; 98: 857872.
28. Şimşek N, Ergün E, Ergün L. Ankara tavşanlarında ekzokrin pankreasın histolojik yapısı. Kafkas Üniv Vet Fak Derg 2009; 15: 173180 (in Turkish).