Sevda ELİŞ YILDIZ, Buket BAKIR, Hamit USLU, Ebru KARADAĞ SARI, Musa KARAMAN

Effects of alpha lipoic acid and vitamin C supplementation on transforming growth factor alpha (TGF a) and transforming growth factor beta 2 (TGF b2)secretion in testicular tissue of diabetic rats

The effects of alpha lipoic acid and vitamin C supplementation on TGF a and TGF b2 secretion in the testicular tissue of streptozotocin-induced diabetic rats were examined by immunohistochemical methods. Rats were divided into 5 groups; group 1 (control), 2 (diabetic control), 3 (diabetes + alpha lipoic acid), 4 (diabetes + vitamin C), and 5 (diabetes + alpha lipoic acid + vitamin C). Hematoxylin and eosin staining was performed in order to examine testicular tissue histopathologically. The streptavidin-biotin-peroxidase complex method was used for histological examination and determination of TGF a and TGF b2 localization in the testicular tissue. Regarding fasting blood glucose levels, the lowest blood glucose level was detected in group 3. Fewer pathological histopathological findings were observed in groups treated with alpha lipoic acid, vitamin C, and alpha lipoic acid + vitamin C compared to the diabetes group. TGF a and TGF b2 secretions increased in groups 3, 4, and 5 compared to group 2. In conclusion, exogenous administration of alpha lipoic acid, vitamin C, and alpha lipoic acid + vitamin C increased TGF a and TGF b2 secretion in streptozotocin-induced diabetic rats.

Anahtar Kelimeler:

Alpha lipoic acid, diabetes, vitamin C, TGF a, TGF b2

Effects of alpha lipoic acid and vitamin C supplementation on transforming growth factor alpha (TGF a) and transforming growth factor beta 2 (TGF b2)secretion in testicular tissue of diabetic rats

Keywords:

Alpha lipoic acid, diabetes, vitamin C, TGF a, TGF b2,

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