Ramesha KEREKOPPA, Marappa BASAVARAJU, Akhila RAO, Gujjar Ramachandra GEETHA, Kataktalware MUKUND, Jeyakumar SAKTHIVEL

Single nucleotide polymorphism identification and characterization of GnRHR gene in Bos taurus and Bos indicus cattle

The hypothalamic gonadotropin-releasing hormone receptor (GnRHR) plays an essential physiological role in reproductive function. In this investigation, we studied genetic variation in the entire coding region of GnRHR gene using PCR-SSCP technique in 250 Bos taurus (Holstein Friesian, Jersey) and Bos indicus (Malnad Gidda, Deoni) bulls. The genetic variants in GnRHR gene were determined by PCR-SSCP technique by using 5 sets of primers. SSCP analysis of fragment 1, comprising exon 1, and fragment 3, comprising exon 2, revealed 2 SSCP patterns in all breeds under study, while fragment 2 of exon 1 and fragment 4, comprising exon 3, revealed 3 patterns in Malnad Gidda; Holstein Friesian and Deoni breeds showed 2 SSCP patterns. In Jersey bulls, fragment 4 showed 3 patterns. The fragment 5 comprising exon 3 was monomorphic in all 4 breeds under study. SNPs were confirmed by direct sequencing. The sequence analysis revealed 7 transitions (T940C, T1021C, T1090C, T1174C, T13940C, T18023C, and G17887A), 3 transversions (G13817C, G17926C, and G17926C), 2 deletions (854DA and 17684DT), and 2 insertions (1009IA and 180100IC) in GnRHR gene. The study revealed high genetic variation in GnRHR gene in cattle.

Anahtar Kelimeler:

GnRHR, PCR-SSCP, single nucleotide polymorphism, cattle

Single nucleotide polymorphism identification and characterization of GnRHR gene in Bos taurus and Bos indicus cattle

Keywords:

GnRHR, PCR-SSCP, single nucleotide polymorphism, cattle,

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