Melatonin receptor 1A gene RsaI and inhibin alpha subunit gene HaeII polymorphisms in Honamli and Hair goat breeds reared in Western Mediterranean region of Turkey
The melatonin receptor 1A (MTNR1A) and inhibin alpha subunit (INHA) genes play a significant role in the reproductive characteristics of animals. Blood samples were collected from 371 goats (Honamli and Hair) reared in Antalya and Burdur. The polymerase chain reaction (PCR) products were digested by RsaI for the MTNR1A gene and HaeII for the INHA gene. Two alleles (A and G) and three genotypes (AA, AG, and GG) were observed for the INHA gene, while two alleles (R and r) and two genotypes (RR and Rr) were observed for the MTNR1A gene. The highest allelic frequency value for G (91.8%) was found in Honamli goat breeds for the INHA gene while the highest value for R (98.1%) was found in Hair goat breeds for the MTNR1A gene. The GG genotype for the INHA gene and the RR genotype for the MTNR1A gene were identified as the most common genotypes of the Honamli and Hair goat breeds. The rr genotype for the MTNR1A gene could not be determined in the breeds. Both Honamli and Hair goat breeds were in Hardy–Weinberg equilibrium for the genes that were studied. In conclusion, this study confirms the existence of genetic polymorphism in the MTNR1A and INHA genes as detected by PCR-RFLP analysis in Honamli and Hair goat breeds.
Melatonin receptor 1A gene RsaI and inhibin alpha subunit gene HaeII polymorphisms in Honamli and Hair goat breeds reared in Western Mediterranean region of Turkey
The melatonin receptor 1A (MTNR1A) and inhibin alpha subunit (INHA) genes play a significant role in the reproductive characteristics of animals. Blood samples were collected from 371 goats (Honamli and Hair) reared in Antalya and Burdur. The polymerase chain reaction (PCR) products were digested by RsaI for the MTNR1A gene and HaeII for the INHA gene. Two alleles (A and G) and three genotypes (AA, AG, and GG) were observed for the INHA gene, while two alleles (R and r) and two genotypes (RR and Rr) were observed for the MTNR1A gene. The highest allelic frequency value for G (91.8%) was found in Honamli goat breeds for the INHA gene while the highest value for R (98.1%) was found in Hair goat breeds for the MTNR1A gene. The GG genotype for the INHA gene and the RR genotype for the MTNR1A gene were identified as the most common genotypes of the Honamli and Hair goat breeds. The rr genotype for the MTNR1A gene could not be determined in the breeds. Both Honamli and Hair goat breeds were in Hardy–Weinberg equilibrium for the genes that were studied. In conclusion, this study confirms the existence of genetic polymorphism in the MTNR1A and INHA genes as detected by PCR-RFLP analysis in Honamli and Hair goat breeds.
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- Hiendleder S, Lewalski H, Jaeger C, Pracht P, Erhardt G. Genomic cloning and comparative sequence analysis of different alleles of the ovine βA-inhibin/activin (INHBA) genes as a potential QTL for litter size. Anim Genet 1996; 27: 91–92.
- Dubocovich ML, Rivera-Bermudez MA, Gerdin MJ, Masan MI. Molecular pharmacology, regulation and function of mammalian melatonin receptors. Front Biosci 2003; 8: 1093– 1108.
- Chu MX, He YY, Cheng DX, Ye SC, Fang L, Wang YY. Association between expression of reproductive seasonality and alleles of melatonin receptor 1A in goats. Anim Reprod Sci 2007; 101: 276–284.
- Carcangiu V, Vacca GM, Mura MC, Dettori M, Pazzola M, Luridiana S, Bini PP. Relationship between MTNR1A melatonin receptor gene polymorphism and seasonal reproduction in different goat breeds. Anim Reprod Sci 2009; 110: 71–78.
- TAGEM. The Presentation Catalogue of Genetic Resources of Turkey’s Domestic Animals. Ankara, Turkey: TAGEM; 2009 (in Turkish).
- Elmaz Ö, Saatci M, Dağ B, Aktaş AH, Ata A, Gülay MŞ, Mamak N, Gök B. Some descriptive characteristics of a new goat breed called Honamli in Turkey. Trop Anim Health Prod 2012; 44: 1913–1920.
- Gök B, Aktaş AH, Dursun Ş. Honamli goat: Rising star of the Taurus Mountains. In: RBI 8th Global Conference on the Conservation of Animal Genetic Resources. Tekirdağ, Turkey; 2011. pp. 65–72.
- Toplu HDO, Altınel A. Some production traits of indigenous Hair goats bred under extensive conditions in Turkey. 1st communication: Reproduction, milk yield and hair production traits of does. Arch Tierz Dummerstorf 2008; 51: 498–506.
- Atay O, Gökdal Ö, Eren V. Reproductive characteristics and kid marketing weights of hair goat flocks in rural conditions in Turkey. Cuban J Agri Sci 2010; 44: 353–358.
- Erten Ö, Yılmaz O. Investigation of reproductive and milk yield traits of Hair Goats raised under extensive conditions. YYÜ Vet Fak Derg 2013; 24: 105–107 (in Turkish with an English abstract).
- Hua GH, Chen SL, Yao HW, Wu WS, Shen Z, Chen QK, Chen L, Wen QY, Yang LG. HaeII RFLP of INHA and its relationship to goat litter size. Hereditas 2007; 29: 972–976.
- Reppert SM, Weaver DR, Ebisawa T. Cloning and characterization of mammalian melatonin receptor that mediates reproductive and circadian responses. Neuron 1994; 13: 1177–1185.
- Yılmaz O, Küçük M, Denk H, Bolacalı M. The effect of adding ram at out of season on fertility of Norduz ewes and survival rate of lambs. YYÜ Vet Fak Derg 2006; 17: 99-102 (in Turkish with an English abstract).
- Akçapınar H. Koyun yetiştiriciliği. 2nd ed. Ankara, Turkey: İsmat Matbaacılık; 2000.
- Lai P, Zhang BY, Wang PQ, Chu MX, Song WJ, Ca BJ. Polymorphism of the melatonin receptor genes and its relationship with seasonal reproduction in the Gulin Ma goat breed. Reprod Dom Anim 2013; 48: 732–737.
- Carcangiu V, Mura MC, Vacca GM, Pazzola M, Dettori ML, Luridiana S, Bini PP. Polymorphism of the melatonin receptor MT1 gene and its relationship with seasonal reproductive activity in the Sarda sheep breed. Anim Reprod Sci 2009; 116: 65–72.
- Chu MX, Cheng DX, Liu WZ, Fang L, Ye SC. Association between melatonin receptor 1A gene and expression of reproductive seasonality in sheep. Asian-Aust J Anim Sci 2006; 19: 1079–1084.
- Carcangiu V, Mura MC, Pazzola M, Vacca GM, Paludo M, Marchi B, Daga C, Bua S, Luridiana S. Characterization of the Mediterranean Italian buffaloes melatonin receptor 1A (MTNR1A) gene and its association with reproductive seasonality. Theriogenology 2011; 76: 419–426.
- Montgomery GW, Galloway SM, Davis GH, McNatty KP. Genes controlling ovulation rate in sheep. Reproduction 2001; 121: 843–852.
- George HD. Major genes affecting ovulation rate in sheep. Genet Sel Evol 2005; 37: 11–23.