Pürhan Barbaros TUNCER, Deniz YENİ, Muhammed Enes İNANÇ, Umut TAŞDEMİR, Mehmet Fuat GÜLHAN, Şükrü GÜNGÖR, Ruhi TÜRKMEN, Fatih AVDATEK

Cryoprotective effect of vaccenic acid supplemented in bull semen extender

The aim of this study was to investigate whether vaccenic acid (VA) added to TRIS semen extender has an effect on freezing bull semen. The semen was collected at intervals of three days through an artificial vagina then pooled. The pooled ejaculates were divided into five components as the control (C; no supplement) and VA (25, 50, 100, and 200 µg / mL) groups then extended with the TRIS-based medium. The samples were left in a cold air cabinet for cooling process (4 °C), then they were equilibrated for 4 h. Afterward, the samples in the groups were frozen by using a programmable freezer. The greatest progressive and total motility results were obtained in the VA25 group (p < 0.001). Besides, the total abnormality and chromatin damage was determined to be on the lowest level in the VA25 group (p < 0.05). Except for VA200, the glutathione level was greater in the treatment groups in comparison to the control group (p < 0.05), while the lowest malondialdehyde was determined in VA25. In conclusion, VA supplemented with TRIS-based extender at the concentration of 25 μg / mL improves the progressive and total motility properties of spermatozoa, reduces the abnormal spermatozoa rate, and has a positive effect on chromatin integrity.

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