Effects of vitamin D administration at the beginning of lactation in dairy cows on inflammatory response and liver metabolism

This study aimed to investigate the effect of vitamin D administered 24 h after calving of dairy cows on calcium-phosphorus metabolism, carbohydrate, lipid and liver metabolism, and inflammatory response. Twenty multiparous Holstein cows were randomly assigned to the experimental (EG) and control group (CG). The EG received 5,000,000 IU vitamin D3 (5 mL) 24 h after parturition intramuscularly, whereas the CG group received 5 mL of 0.9% NaCl. Blood samples were taken 24 h after parturition before treatments and at the 3rd, 7th, 14th, and 28th days of lactation. Serum NEFA, BHBA, haptoglobin, serum amyloid A (SAA), ceruloplasmin, calcium, phosphorus, parathormone, total protein, triglyceride, AST, and GGT were measured. In addition, total bilirubin, albumin, total cholesterol, and vitamin A concentrations were also measured to calculate liver activity index and the liver functionality index. Subclinical hypocalcemia (SCH) was observed in only one cow in the EG and five in the CG after the 3rd day postpartum. NEFA levels in the EG significantly differed on days 7, 14, and 28 when compared to vitamin D pretreatment values. However, NEFA levels in the CG differed only between day 28 postpartum and the 1st day (p < 0.05). Negative correlation was determined between vitamin D and NEFA (p < 0.01). Conversely, negative correlations were observed between calcium and NEFA, haptoglobin, SAA (p < 0.05). In conclusion, it can be suggested that vitamin D administration can prevent SCH after the 3rd day and has limited positive effects on postpartum NEFA levels.

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1. Leblanc S. Monitoring metabolic health of dairy cattle in the transition period. The Journal of reproduction and development 2010; 56: 29-35.
2. Murray RD, Horsfield JD, McCormick WD, Williams H, Ward D. Historical and current perspectives on the treatment, control and pathogenesis of milk fever in dairy cattle. Veterinary Record 2008; 163: 561-565.
3. Martinez N, Risco CA, Lima FS, Bisinotto RS, Greco LF et al. Evaluation of peripartal calcium status, energetic profile, and neutrophil function in dairy cows at low or high risk of developing uterine disease. Journal of Dairy Science 2012; 95: 7158-7172.
4. Bertoni G, Trevisi E. Use of the liver activity index and other metabolic variables in the asssesment of metabolic health in dairy herds. Veterinary Clinics North America Food Animal 2013; 29: 413-431.
5. Martinez N, Sinedino LDP, Bisinotto RS, Ribeiro ES, Gomes GC et al. Effect of induced subclinical hypocalcemia on physiological responses and neutrophil function in dairy cows. Journal of Dairy Science 2014; 97: 874-887.
6. Trevisi E, Minuti A. Assessment of the innate immune response in the periparturient cow. Research Veterinary Science 2018; 116: 47-54.
7. Ceciliani F, Giordano A, Spagnolo V. The systemic reaction during inflammation: the acute phase proteins. Protein and Peptide Letters 2002; 9: 211–23.
8. Ceciliani F, Ceron JJ, Eckersall PD, Saurwein H. Acute-phase proteins in ruminants. Journal of Proteomics 2012; 75: 4207- 4231.
9. Ulutas PA, Ulutas B, Kiral F, Asici GSE, Gültekin M. Changes of acute phase protein levels in Saanen goat kids during neonatal period. Small Ruminant Research 2017; 146: 33-36.
10. Horst RL, Goff JP, Reinhardt TA. Role of vitamin D in calcium homeostasis and its use in prevention of bovine periparturient paresis. Acta Veterinaria Scandinavica Supplement 2003; 97: 35-50.
11. Goff JP. Calcium and magnesium disorders. Veterinary Clinics North America Food Animal 2014; 30: 359-381.
12. Krueger LA, Reinhardt TA, Beitz DC, Stuart RL, Stabel JL. Effects of fractionated colostrum replacer and vitamins A, D, and E on haptoglobin and clinical health in neonatal Holstein calves challenged with Mycobacterium avium ssp. Paratuberculosis. Journal of Dairy Science 2016; 99: 2884-2895.
13. Julien WE, Conrad HR, Hibbs JW, Christ WL. Milk fever in dairy cows. VIII. Effect of injected vitamin D3 and calciumand phosphorus intake on incidence. Journal of Dairy Science 1977; 60: 431-436.
14. Veskoukis AS, Kyparos A., Paschalis V., Nikolaidis MG. Spectrophotometric assays for measuring redox biomarkers in blood. Biomarkers 2016; 21:208-217.
15. Enko D, Krieghauser G, Stolba R, Worf E, Halwacs-Bauman G. Method evaluation study of a new generation of vitamin D assays. Biochemia Medica 2015; 25: 203-212.
16. Caixeta LS, Ospina PA, Capel MB, Nydam DV. The association of subclinical hypocalcemia, negative energy balance and disease with bodyweight change during the first 30 days postpartum in dairy cows milked with automatic milking systems. The Veterinary Journal 2015; 204: 150-156.
17. Gillet M, Kirsch P, Besnier P, Millemann Y. Subclinical hypocalcemia in French dairy cows: Study in a veterinary practice in the department of the Ardennes. In: 29 th World Buiatrics Congress, Dublin, Ireland; 2016. pp 180.
18. Vieira-Neto A, Lima RP, Lopes F, Lopera C, Zimpel L et al. Use of calcitriol to maintain postpartum blood calcium and improve immune function in dairy cows. Journal of Dairy Science 2017; 100: 5805-5823.
19. Radostits OM, Gay CC, Hindcliff K, Constable P. Metabolic predictors of left side displacement of abomasum. In: Radostits OM (editor) Veterinary Medicine, 10th ed. Oxford University Press, New York, USA; 2007. pp. 357-360.
20. Mezzetti M, Bionaz M, Trevisi E. Interaction between inflammation and metabolism in periparturient dairy cows. Journal of Animal Science 2020; 98: 155–174.
21. Farid SA, Honkawa K, Fath ME, Nonaka N, Horii Y. Serum paraoxonase-1 as marker improved diagnosis of fatty liver in dairy cows. BMC Veterinary Research 2013; 9-73.
22. LeBlanc SJ, Leslie KE, Duffield TF. Metabolic predictors of displaced abomasum in dairy cattle. Journal of Dairy Science 2005; 88: 159-170.
23. Kvidera SK, Dickson MJ, Abuajamieh M, Snider DB, Sanz Fernandez MV et al. Intentionally induced intestinal barrier dysfunction causes inflammation, affects metabolism, and reduces productivity in lactating Holstein cows. Journal of Dairy Science 2017; 100: 4113-4127.
24. McCarthy M, Mann MS, Nydam DV, Overton TR, McArt, JA. Short communication: concentrations of nonesterified fatty acids and β-hydroxybutyrate in dairy cows are not well correlated during the transition period. Journal of Dairy Science 2015; 98: 6284-6290.
25. Nelson CD, Lippolis JD, Reinhardt TA, Sacco RE, Powell JL. Vitamin D status of dairy cattle: Outcomes of current practices in the dairy industry. Journal of Dairy Science 2016; 99: 10150- 10160.
26. Casas EJ, Lippolis D, Kuehn LA, Reinhardt TA. Seasonal variation in vitamin D status of beef cattle reared in the central United States. Domestic Animal Endocrinology 2015; 52, 71- 74.
27. Barchetta I, Angelico F, Del Ben M, Baroni MG, Pozzilli P et al. Strong association between non alcoholic fatty liver disease (NAFLD) and low 25(OH) vitamin D levels in an adult population with normal serum liver enzymes. BMC Medicine 2011; 9: 85.
28. Zhou QG, Hou FF, Guo ZJ, Liang M, Wang GB. 1,25- Dihydroxyvitamin D improved the free fatty-acid-induced insulin resistance in cultured C2C12 cells. Diabetes/ Metabolism Research and Reviews 2004; 24: 459-464.
29. Sadek MK, Shaheen H. Biochemical efficacy of vitamin D in ameliorating endocrine and metabolic disorders in diabetic rats. Journal Pharmaceutical Biology 2013; 52: 591-596.
30. Trevisi E, Amadori M, Cogrossi S, Razzuoli E, Bertoni G. Metabolic stress and inflammatory response in high-yielding, periparturient dairy cows. Research in Veterinary Science 2012; 93, 695-704.
31. Bossaert P, Trevisi E, Opsomer G, Bertoni G, De Vliegher Z, et al. The association between indicators of inflammation and liver variables during the transition period in high-yielding dairy cows: An observational study. The Veterinary Journal 2012; 192: 222-225.
32. Bertoni G, Trevisi E, Han X, Bionaz M. Effects of inflammatory conditions on liver activity in puerperium period and consequences for performance in dairy cows. Journal of Dairy Science 2008; 91: 3300- 3310.
33. Martinez N, Sinedino LDP, Bisinotto RS, Ribeiro ES, Gomes GC et al. Effect of induced subclinical hypocalcemia on physiological responses and neutrophil function in dairy cows. Journal of Dairy Science 2014; 97: 874-887.
34. Fleck A. Clinical and nutritional aspects of changes in acute phase proteins during inflammation. The Proceedings of the Nutrition Society 1989; 48: 347– 354.
35. Zhang G, Hailemariam D, Dervishi E, Goldansaz SA, Deng Q et al. Dairy cows affected by ketosis show alterations in innate immunity and lipid and carbohydrate metabolism during the dry off period and postpartum. Research in Veterinary Science 2016; 107: 246-256.
36. Ametaj B, Nonnecke B, Horst R, Beitz D. Effects of retinoic acid and 1, 25- dihydroxyvitamin D3 on IFN-γ secretion by mononuclear leukocytes from nulliparous and postparturient dairy cattle. International Journal for Vitamin and Nutrition Research 2000; 70: 92-101.