The role of hepcidin and its related genes (BMP6, GDF-15, and HJV) in rats exposed to ischemia and reperfusion

To determine the roles of hepcidin and its related genes in a renal ischemia/reperfusion model. Materials and methods: A total of 20 Wistar albino rats were equally divided into 2 groups: Group I was the control group and Group II was the ischemia and reperfusion (I/R) group (60 min of ischemia + 48 h of reperfusion). I/R was performed on the left kidneys of these rats and then the I/R-treated kidneys were removed. The levels of serum biochemical markers were evaluated after renal I/R. The expression levels of hepcidin-linked genes [growth differentiation factor 15 (GDF-15), bone morphogenetic protein 6 (BMP6), and hemojuvelin (HJV)] were also measured by RT-PCR technique. In addition, the tissues were evaluated histopathologically. Results: No significant association was found between renal dysfunction and I/R when compared to biochemical parameters (P > 0.05). However, differences in platelet values were statistically significant (P < 0.05). Expression levels of GDF-15, BMP6, and HJV genes increased, but this increase was not statistically significant. In addition, histopathological evaluation was performed using hematoxylin and eosin stain. This showed a significant relationship between the control group and I/R group for ischemic and nonischemic kidney scoring. Conclusion: Hepcidin and BMP6, HJV, and GDF-15 should be taken into account when investigating the process of I/R.

The role of hepcidin and its related genes (BMP6, GDF-15, and HJV) in rats exposed to ischemia and reperfusion

To determine the roles of hepcidin and its related genes in a renal ischemia/reperfusion model. Materials and methods: A total of 20 Wistar albino rats were equally divided into 2 groups: Group I was the control group and Group II was the ischemia and reperfusion (I/R) group (60 min of ischemia + 48 h of reperfusion). I/R was performed on the left kidneys of these rats and then the I/R-treated kidneys were removed. The levels of serum biochemical markers were evaluated after renal I/R. The expression levels of hepcidin-linked genes [growth differentiation factor 15 (GDF-15), bone morphogenetic protein 6 (BMP6), and hemojuvelin (HJV)] were also measured by RT-PCR technique. In addition, the tissues were evaluated histopathologically. Results: No significant association was found between renal dysfunction and I/R when compared to biochemical parameters (P > 0.05). However, differences in platelet values were statistically significant (P < 0.05). Expression levels of GDF-15, BMP6, and HJV genes increased, but this increase was not statistically significant. In addition, histopathological evaluation was performed using hematoxylin and eosin stain. This showed a significant relationship between the control group and I/R group for ischemic and nonischemic kidney scoring. Conclusion: Hepcidin and BMP6, HJV, and GDF-15 should be taken into account when investigating the process of I/R.

Kaynakça

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Kaynak Göster

Bibtex @araştırma makalesi { tbtkmedical146664, journal = {Turkish Journal of Medical Sciences}, issn = {1300-0144}, eissn = {1303-6165}, address = {}, publisher = {TÜBİTAK}, year = {2014}, volume = {44}, pages = {576 - 581}, doi = {10.3906/kim-1210-56}, title = {The role of hepcidin and its related genes (BMP6, GDF-15, and HJV) in rats exposed to ischemia and reperfusion}, key = {cite}, author = {Dokuyucu, Recep and Demir, Tuncer and Yumrutaş, Önder and Erbağcı, Ayşe Binnur and Örkmez, Mustafa} }
APA Dokuyucu, R , Demir, T , Yumrutaş, Ö , Erbağcı, A , Örkmez, M . (2014). The role of hepcidin and its related genes (BMP6, GDF-15, and HJV) in rats exposed to ischemia and reperfusion . Turkish Journal of Medical Sciences , 44 (4) , 576-581 . DOI: 10.3906/kim-1210-56
MLA Dokuyucu, R , Demir, T , Yumrutaş, Ö , Erbağcı, A , Örkmez, M . "The role of hepcidin and its related genes (BMP6, GDF-15, and HJV) in rats exposed to ischemia and reperfusion" . Turkish Journal of Medical Sciences 44 (2014 ): 576-581 <
Chicago Dokuyucu, R , Demir, T , Yumrutaş, Ö , Erbağcı, A , Örkmez, M . "The role of hepcidin and its related genes (BMP6, GDF-15, and HJV) in rats exposed to ischemia and reperfusion". Turkish Journal of Medical Sciences 44 (2014 ): 576-581
RIS TY - JOUR T1 - The role of hepcidin and its related genes (BMP6, GDF-15, and HJV) in rats exposed to ischemia and reperfusion AU - Recep Dokuyucu , Tuncer Demir , Önder Yumrutaş , Ayşe Binnur Erbağcı , Mustafa Örkmez Y1 - 2014 PY - 2014 N1 - doi: 10.3906/kim-1210-56 DO - 10.3906/kim-1210-56 T2 - Turkish Journal of Medical Sciences JF - Journal JO - JOR SP - 576 EP - 581 VL - 44 IS - 4 SN - 1300-0144-1303-6165 M3 - doi: 10.3906/kim-1210-56 UR - Y2 - 2021 ER -
EndNote %0 Turkish Journal of Medical Sciences The role of hepcidin and its related genes (BMP6, GDF-15, and HJV) in rats exposed to ischemia and reperfusion %A Recep Dokuyucu , Tuncer Demir , Önder Yumrutaş , Ayşe Binnur Erbağcı , Mustafa Örkmez %T The role of hepcidin and its related genes (BMP6, GDF-15, and HJV) in rats exposed to ischemia and reperfusion %D 2014 %J Turkish Journal of Medical Sciences %P 1300-0144-1303-6165 %V 44 %N 4 %R doi: 10.3906/kim-1210-56 %U 10.3906/kim-1210-56
ISNAD Dokuyucu, Recep , Demir, Tuncer , Yumrutaş, Önder , Erbağcı, Ayşe Binnur , Örkmez, Mustafa . "The role of hepcidin and its related genes (BMP6, GDF-15, and HJV) in rats exposed to ischemia and reperfusion". Turkish Journal of Medical Sciences 44 / 4 (Mayıs 2014): 576-581 .
AMA Dokuyucu R , Demir T , Yumrutaş Ö , Erbağcı A , Örkmez M . The role of hepcidin and its related genes (BMP6, GDF-15, and HJV) in rats exposed to ischemia and reperfusion. Turkish Journal of Medical Sciences. 2014; 44(4): 576-581.
Vancouver Dokuyucu R , Demir T , Yumrutaş Ö , Erbağcı A , Örkmez M . The role of hepcidin and its related genes (BMP6, GDF-15, and HJV) in rats exposed to ischemia and reperfusion. Turkish Journal of Medical Sciences. 2014; 44(4): 576-581.
IEEE R. Dokuyucu , T. Demir , Ö. Yumrutaş , A. Erbağcı ve M. Örkmez , "The role of hepcidin and its related genes (BMP6, GDF-15, and HJV) in rats exposed to ischemia and reperfusion", Turkish Journal of Medical Sciences, c. 44, sayı. 4, ss. 576-581, May. 2014, doi:10.3906/kim-1210-56
  • ISSN: 1300-0144
  • Yayın Aralığı: Yılda 6 Sayı
  • Yayıncı: TÜBİTAK

24.7b 23.8b

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