Pharmacological profile of a nitric oxide donor spermine NONOate in the mouse corpus cavernosum

To investigate the effects of spermine NONOate in the cavernous tissue obtained from mice treated or untreated with sildenafil. Materials and methods: We studied the effects of spermine NONOate on the tone and nitrergic relaxation responses of isolated mouse corpus cavernosum and compared them with sodium nitroprusside in the absence or presence of L-nitroarginine, hydroxocobalamin, pyrogallol, diethyldithiocarbamate (DETCA), or 1H-[1,2,4]Oxadiazolo[4,3-a]quinoxalin-1-one (ODQ). The neurogenic contractions and relaxations of the tissues were induced by electrical field stimulation. Some mice received a single oral dose of sildenafil and after 1 h the effects of spermine NONOate were evaluated by in vitro studies. Results: Spermine NONOate relaxed mouse corpus cavernosum in a concentration-dependent manner. Spermine-NONOate-induced relaxation was relatively slow to develop and it was reversible and reproducible. These relaxations were significantly suppressed by hydroxocobalamin, diethyldithiocarbamate, or ODQ, but not by L-nitroarginine or pyrogallol. Spermine NONOate potentiated the nitrergic relaxations to electrical field stimulation (EFS), whereas it significantly reduced EFS-induced contractions. Sildenafil treatment can enhance the relaxant responses to spermine NONOate and EFS. Conclusion: These findings suggest that spermine NONOate has a potent relaxant action in cavernous tissue and this effect can be potentiated by oral sildenafil treatments. Spermine NONOate may be considered an attractive treatment for erectile dysfunction in pathologic disorders with a lack of endogenous NO production.

Pharmacological profile of a nitric oxide donor spermine NONOate in the mouse corpus cavernosum

To investigate the effects of spermine NONOate in the cavernous tissue obtained from mice treated or untreated with sildenafil. Materials and methods: We studied the effects of spermine NONOate on the tone and nitrergic relaxation responses of isolated mouse corpus cavernosum and compared them with sodium nitroprusside in the absence or presence of L-nitroarginine, hydroxocobalamin, pyrogallol, diethyldithiocarbamate (DETCA), or 1H-[1,2,4]Oxadiazolo[4,3-a]quinoxalin-1-one (ODQ). The neurogenic contractions and relaxations of the tissues were induced by electrical field stimulation. Some mice received a single oral dose of sildenafil and after 1 h the effects of spermine NONOate were evaluated by in vitro studies. Results: Spermine NONOate relaxed mouse corpus cavernosum in a concentration-dependent manner. Spermine-NONOate-induced relaxation was relatively slow to develop and it was reversible and reproducible. These relaxations were significantly suppressed by hydroxocobalamin, diethyldithiocarbamate, or ODQ, but not by L-nitroarginine or pyrogallol. Spermine NONOate potentiated the nitrergic relaxations to electrical field stimulation (EFS), whereas it significantly reduced EFS-induced contractions. Sildenafil treatment can enhance the relaxant responses to spermine NONOate and EFS. Conclusion: These findings suggest that spermine NONOate has a potent relaxant action in cavernous tissue and this effect can be potentiated by oral sildenafil treatments. Spermine NONOate may be considered an attractive treatment for erectile dysfunction in pathologic disorders with a lack of endogenous NO production.

Kaynakça

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Kaynak Göster

Bibtex @ { tbtkmedical146663, journal = {Turkish Journal of Medical Sciences}, issn = {1300-0144}, eissn = {1303-6165}, address = {}, publisher = {TÜBİTAK}, year = {2014}, volume = {44}, pages = {569 - 575}, doi = {10.3906/muh-1207-21}, title = {Pharmacological profile of a nitric oxide donor spermine NONOate in the mouse corpus cavernosum}, key = {cite}, author = {Ertuğ, Fatma Peyman and Şingirik, Erğin and Büyüknacar, Hacer Sinem and Göçmen, Cemil and Seçilmiş, Mehmet Ata} }
APA Ertuğ, F , Şingirik, E , Büyüknacar, H , Göçmen, C , Seçilmiş, M . (2014). Pharmacological profile of a nitric oxide donor spermine NONOate in the mouse corpus cavernosum . Turkish Journal of Medical Sciences , 44 (4) , 569-575 . DOI: 10.3906/muh-1207-21
MLA Ertuğ, F , Şingirik, E , Büyüknacar, H , Göçmen, C , Seçilmiş, M . "Pharmacological profile of a nitric oxide donor spermine NONOate in the mouse corpus cavernosum" . Turkish Journal of Medical Sciences 44 (2014 ): 569-575 <
Chicago Ertuğ, F , Şingirik, E , Büyüknacar, H , Göçmen, C , Seçilmiş, M . "Pharmacological profile of a nitric oxide donor spermine NONOate in the mouse corpus cavernosum". Turkish Journal of Medical Sciences 44 (2014 ): 569-575
RIS TY - JOUR T1 - Pharmacological profile of a nitric oxide donor spermine NONOate in the mouse corpus cavernosum AU - Fatma Peyman Ertuğ , Erğin Şingirik , Hacer Sinem Büyüknacar , Cemil Göçmen , Mehmet Ata Seçilmiş Y1 - 2014 PY - 2014 N1 - doi: 10.3906/muh-1207-21 DO - 10.3906/muh-1207-21 T2 - Turkish Journal of Medical Sciences JF - Journal JO - JOR SP - 569 EP - 575 VL - 44 IS - 4 SN - 1300-0144-1303-6165 M3 - doi: 10.3906/muh-1207-21 UR - Y2 - 2021 ER -
EndNote %0 Turkish Journal of Medical Sciences Pharmacological profile of a nitric oxide donor spermine NONOate in the mouse corpus cavernosum %A Fatma Peyman Ertuğ , Erğin Şingirik , Hacer Sinem Büyüknacar , Cemil Göçmen , Mehmet Ata Seçilmiş %T Pharmacological profile of a nitric oxide donor spermine NONOate in the mouse corpus cavernosum %D 2014 %J Turkish Journal of Medical Sciences %P 1300-0144-1303-6165 %V 44 %N 4 %R doi: 10.3906/muh-1207-21 %U 10.3906/muh-1207-21
ISNAD Ertuğ, Fatma Peyman , Şingirik, Erğin , Büyüknacar, Hacer Sinem , Göçmen, Cemil , Seçilmiş, Mehmet Ata . "Pharmacological profile of a nitric oxide donor spermine NONOate in the mouse corpus cavernosum". Turkish Journal of Medical Sciences 44 / 4 (Mayıs 2014): 569-575 .
AMA Ertuğ F , Şingirik E , Büyüknacar H , Göçmen C , Seçilmiş M . Pharmacological profile of a nitric oxide donor spermine NONOate in the mouse corpus cavernosum. Turkish Journal of Medical Sciences. 2014; 44(4): 569-575.
Vancouver Ertuğ F , Şingirik E , Büyüknacar H , Göçmen C , Seçilmiş M . Pharmacological profile of a nitric oxide donor spermine NONOate in the mouse corpus cavernosum. Turkish Journal of Medical Sciences. 2014; 44(4): 569-575.
IEEE F. Ertuğ , E. Şingirik , H. Büyüknacar , C. Göçmen ve M. Seçilmiş , "Pharmacological profile of a nitric oxide donor spermine NONOate in the mouse corpus cavernosum", Turkish Journal of Medical Sciences, c. 44, sayı. 4, ss. 569-575, May. 2014, doi:10.3906/muh-1207-21
  • ISSN: 1300-0144
  • Yayın Aralığı: Yılda 6 Sayı
  • Yayıncı: TÜBİTAK

24.8b 23.8b

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