Mikdat BOZER, Ayhan AKPINAR, Meral ŞEN, Önder SÜRGİT, Aydın İNAN

Etofenamate and anastomoses of the colon in rats

Aim: Non-steroidal anti-inflammatory drugs have been used for many years due to their analgesic and anti-inflammatory properties. They have also been used for postoperative analgesia. We aimed to study the effects of etofenamate, a non-steroidal anti-inflammatory drug, on the healing of colonic anastomoses in rats. Materials and methods: Sprague-Dawley rats were used in this study. Resection and anastomosis were performed on the distal colon. The etofenamate group received 30 mg/kg etofenamate and the control group received 0.1 mL of 0.9% NaCl intramuscularly daily. Bursting pressures of anastomoses and hydroxyproline levels of perianastomotic tissues were determined on days 3 and 7. Results: Four deaths were observed in each group. Mean bursting pressures (etofenamate group, day 3: 50.50 ± 7.27 mmHg, day 7: 187.37 ± 12.20 mmHg; control group, day 3: 55.13 ± 5.94 mmHg, day 7: 202.12 ± 18.64 mmHg) and mean hydroxyproline levels (etofenamate group, day 3: 2.18 ± 0.17 µg/mg tissue, day 7: 4.34 ± 0.79 µg/mg tissue; control group, day 3: 2.20 ± 0.12 µg/mg tissue, day 7: 5.07 ± 0.65 µg/mg tissue) in the etofenamate group were lower than those in the control group on both days 3 and 7. This finding was not statistically significant. Conclusion: According to our findings, etofenamate treatment during the postoperative period does not alter the healing of colonic anastomoses in rats.

Anahtar Kelimeler:

Key words: Non-steroidal anti-inflammatory drugs, etofenamate, colon, anastomosis, healing, rat

Etofenamate and anastomoses of the colon in rats

Keywords:

Key words: Non-steroidal anti-inflammatory drugs, etofenamate, colon, anastomosis, healing, rat,

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Tseng CC, Wolfe MM. Nonsteroidal anti-inflammatory drugs. Med Clin N Am 2000; 84: 1329-44.
Cali RL, Smyrk TC, Blatchford GJ, Thorson AG, Christensen MA. Effect of prostaglandin E1 and steroid on healing colonic anastomosis. Dis Colon Rectum 1993; 36: 1148-51.
Jamall IS, Finelli VN, Que Hee SS. A simple method to determine nanogram levels of 4-hydroxyproline in biological tissues. Anal Biochem 1981; 112: 70-5.
Harder AT, An YH. The mechanisms of the inhibitory effects of nonsteroidal anti-inflammatory drugs on bone healing: a concise review. J Clin Pharmacol 2003; 43: 807-15.
Quirinia A, Viidik A. Diclofenac and indomethacin influence the healing of normal and ischemic incisional wounds in skin. Scand J Plast Reconstr Hand Surg 1997; 31: 213-9.
Hegi TR, Bombeli T, Seifert B, Baumann PC, Haller U, Zalunardo MP et al. Effect of refocoxib on platelet aggregation and blood loss in gynaecological and breast surgery compared with diclofenac. Br J Anaesth 2004; 92: 523-31.
Housby JN, Cahill CM, Chu B, Prevelige R, Bickford K, Stevenson MA, Calderwood SK. Non-steroidal anti- inflammatory drugs inhibit the expression of cytokines and induce HSP70 in human monocytes. Cytokine 1999; 11: 347- 58.
Hinz B, Rau T, Auge D, Werner U, Ramer R, Rietbrock S, Brune K. Aceclofenac spares cyclooxygenase 1 as a result of limited but sustained biotransformation to diclofenac. Clin Pharm Ther 2003; 74: 222-35.
Blomme EAG, Cinn KS, Hardy MM, Casler JJ, Kim SH, Opsahl AC et al. Selective cyclooxygenase-2 inhibition does not affect the healing of cutaneous full-thickness incisional wounds in SKH-1 mice. Br J Dermatol 2003; 148: 211-23.
Ohwada S, Sato Y, Sato N, Toyama Y, Okano T, Nakasone Y et al. Effects of transfusion on gastrointestinal anastomotic wound healing and leukocyte function in rats. Eur Surg Res 2000; 32: 353-8.
Chithra P, Sajithlal GB, Chandrakasan G. Influence of Aloe vera on collagen characteristics in healing dermal wounds in rats. Molec Cellular Biochem 1998; 181: 71-6.
De Sousa JB, Soares AG, Francisco A. Effects of diclofenac sodium on intestinal anastomotic healing. Dis Colon Rectum 1991; 34: 613-7.
Angelis-Stoforidis P, Vajda FJE, Christophidis N. Effect of non- steroidal anti-inflammatory drugs (NSAIDs) on human polymorphonuclear leucocyte function in buffer and plasma. Clin Exp Rheumatol 1998; 16: 703-8.
Hirasawa N, Ohuchi K, Watanabe M, Tsurufuji S. Mechanism of the inhibitory action of cyclooxygenase inhibitors on leukocyte infiltration: involvement of endogenous histamine. European J Pharmacol 1987; 144: 267-75.
Månsson P, Zhang XW, Jeppsson B, Thorlacius H. Anastomotic healing in the rat colon: comparison between a radiological method, breaking strength and bursting pressure. Int J Colorectal Dis 2002; 17: 420-5.
Witte MB, Barbul A. General principles of wound healing. Surg Clin N Am 1997; 77: 509-28.
Facino RM, Carini M, Saibene L. Scavenging of free radicals by tenoxicam: a participating mechanism in the antirheumatic/antiinflammatory efficacy of the drug. Arch Pharm Pharm Med Chem 1996; 329: 457-63.
Hendriks JMH, Hubens G, Wuyts FL, Vermeulen P, Hubens A, Eyskens E. Experimental study of intraperitoneal suramin on the healing of colonic anastomoses. Br J Surg 1999; 86: 1171-5.
Mall JW, Schwemk W, Philipp AW, Büttemeyer R, Pollmann C. Intraperitoneal administration of the angiogenesis inhibitor thalidomide does not impair anastomotic healing following large bowel resection in a rabbit model. World J Surg 2003; 27: 1119-23.
Stumpf M, Cao W, Klinge U, Klosterhalfen B, Kasperk R, Schumpelick V. Collagen distribution and expression of matrix metalloproteinases 1 and 13 in patients with anastomotic leakage after large-bowel surgery. Langenbeck’s Arch Surg 2002; 386: 502-6.
Pesakova V, Gillery P, Maquart FX, Borel JP, Adam M. The influence of some non-steroidal anti-inflammatory drugs on the retraction of collagen lattices. Biomed Pharmacother 1991; 45: 455-9.
Seyer-Hansen M, Andreassen TT, Cristensen H, Oxlund H. Effect of experimental diabetes and growth hormone administration on the strength of colonic anastomoses in rats. Eur Surg Res 1999; 31: 419-28.