Zeliha DEMİREL, Ferda FETHİYE YILMAZ, Güven ÖZDEMİR, Meltem Conk DALAY

Influence of Media and Temperature on the Growth and the Biological Activities of Desmodesmus protuberans (F.E. Fritsch & M.F. Rich) E. Hegewald

The aim of this study is to determine different culture media and temperature values on the growth rate of Desmodesmusprotuberans (F.E. Fritsch & M.F.Rich) E. Hegewald, and to investigate antimicrobial and antioxidant potency of the crudeextracts. The microalga isolates from Lake Eğirdir in Isparta (Turkey) was identified as D. protuberans and included in EgeUniversity Microalgae Culture Collection. D. protuberans was cultivated at two temperature regimes (22 and 28ºC) andgrowth media (BBM, BG-11 and RD medium) under the light intensity of 75 μmol m-2s-1 for testing the impact of differentenvironmental conditions on the growth rate. All of the productions of D. protuberans biomass were extracted with methanoland investigated the antimicrobial and antioxidant activity. Antimicrobial activities of the extracts of alga were tested by discdiffusion assay against five Gram-positive and four Gram-negative bacterial strains and against the yeast Candida albicans.The methanol extract of the biomass from BBM medium (28°C) showed the highest growth inhibition effect on C. albicans,while RD medium (22°C) extract showed the highest antioxidant activity. These results demonstrated that the differences inthe bioactivity of biomasses are stemmed from different temperature and media conditions.

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Abedin, R.M.A., & Taha, H.M. (2008). Antibacterial and antifungal activity of cyanobacteria and green microalgae. Evaluation of medium components by Plackett Burman design for antimicrobial activity of Spirulina platensis. Global Journal of Biotechnology and Biochemistry, 3(1), 22-31.

Akgül, R. (2017). Culture of Desmodesmus communis (E. Hegewald) E. Hegewald and Its Determination of the Biochemical Properties. Turkish Journal of Agriculture-Food Science and Technology, 5(4), 404- 408. https://doi.org/10.24925/turjaf.v5i4.404- 408.1254

Akgül, F., Kizilkaya, İ.T., Akgül, R., & Erduğan, H. (2017). Morphological and Molecular Characterization of Scenedesmus-Like Species from Ergene River Basin (Thrace, Turkey). Turkish Journal of Fisheries and Aquatic Sciences, 17(3), 609-619. https://dx.doi.org/10.4194/1303-2712-v17_3_17

Bradshaw, L. J. (1992). Laboratory Microbiology, Emeritus California State University, Fullerton.

Benavente-Valdés, J.R., Aguilar, C., Contreras-Esquivel, J.C., Méndez-Zavala, A., & Montañez, J. (2016). Strategies to enhance the production of photosynthetic pigments and lipids in chlorophycae species. Biotechnology Reports, 10, 117-125. https://dx.doi.org/10.1016/j.btre.2016.04.001

Bourrelly, P. (1962). Ulotricales d’eau douce rares ou nouvelles. Phykos, 1, 29-35.

Bourrelly, P. (1972). Les algues d'eau douce. Initiation à la Systématique: Tome I. Les algues vertes. Société Nouvelle des Éditions Boubée.

Collins C.M., & Lyne P.M. (1987): Microbiological Methods. London, Butterworths & Co. Ltd., 316 pp.

Demirel, Z., Yilmaz-Koz, F. F., Karabay-Yavasoglu, U. N.,Ozdemir, G., & Sukatar, A. (2009). Antimicrobial and antioxidant activity of brown algae from the Aegean Sea. Journal of the Serbian Chemical Society, 74(6), 619-628. https://dx.doi.org/10.2298/JSC0906619D

Demirel, Z., Yilmaz-Koz, F. F., Karabay-Yavasoglu, N. U., Ozdemir, G., & Sukatar, A. (2011). Antimicrobial and antioxidant activities of solvent extracts and the essential oil composition of Laurencia obtusa and Laurencia obtusa var. pyramidata. Romanian biotechnological letters, 16(1), 5927-5936.

El-Sayed, A.B., & Abdel-Maguid, A.A. (2010). Immobilized-microalga Scenedesmus sp. for Biological Desalination of Red Sea Water: II. Effect on Macronutrients Removal. Journal of American Science, 6(9), 637-643.

Erdoğan, A., Çağır, A., Dalay, M. C., & Eroğlu, A. E. (2015). Composition of Carotenoids in Scenedesmus protuberans: Application of Chromatographic and Spectroscopic Methods. Food Analytical Methods, 8, 1970-1978. https://dx.doi.org/10.1007/s12161-015- 0088-8

Ghasemi, Y., Moradian, A., Mohagheghzadeh, A., Shokravi, S., & Morowvat, M.H. (2007). Antifungal and Antibacterial Activity of the Microalgae Collected from Paddy Fields of Iran: Characterization of Antimicrobial Activity of Chroococcus dispersus. Journal of Biological Sciences, 7(6), 904-9010.

Goh, S.H., Yusoff, F.M.,& Loh, S.P.A. (2010). A comparison of the antioxidant properties and total phenolic content in a diatom Chaetoceros sp. and a green microalga, Nannochloropsis sp. Journal of Agricultural Science, 2, 123-130. http://dx.doi.org/10.5539/jas.v2n3p123

Goiris, K., Colen, W.V., Wilches, I., León-Tamariz, F., Cooman, L.D., & Muylaert, K. (2015). Impact of nutrient stress on antioxidant production in three species of microalgae. Algal Research, 7, 51-57. https://dx.doi.org/10.1016/j.algal.2014.12.002

Imamoglu, E., Demirel, Z., & Dalay, M.C. (2014). Evaluation of culture conditions of locally isolated Dunaliella salina strain EgeMacc-024. Biochemical Engineering Journal, , 22-27. https://dx.doi.org/10.1016/j.bej.2014.05.008

Karło, A., Wilk, A., Ziembińska-Buczyńska, A., & Surmacz-Gorska, J. (2015). Cultivation Parameters Adjustment for Effective Algal Biomass Production. Rocznik Ochrona Środowiska, 17(cz. 1), 275-288.

Koz, F.F.Y., Yavasoglu, N.U.K., Demirel, Z., Sukatar, A., & Ozdemir, G. (2009). Antioxidant and Antimicrobial Activities of Codium fragile (Suringar) Hariot (Chlorophyta) Essential Oil and Extracts. Asian Journal of Chemistry, 21(2), 1197-1209.

Li, B., Cheng, K., Wong, C., Fan, K., Chen, F., & Jiang, Y. (2007). Evaluation of antioxidant capacity and total phenolic content of different fractions of selected microalgae. Food Chemistry, 102, 771-776. https://dx.doi.org/10.1016/j.foodchem.2006.06.022

Maadane, A., Merghoub, N., Ainane, T., Arroussi, H.E., Benhima, R., Amzazi, S., Bakri, Y., & Wahby, I. (2015). Antioxidant activity of some Moroccan marine microalgae: Pufa profiles, carotenoids and phenolic content. Journal of Biotechnology, 215, 13- 19. https://dx.doi.org/10.1016/j.jbiotec.2015.06.400.

Mann, G., Schlegel, M., Schumann, R., & Sakalauskas, A. (2009). Biogas-conditioning with microalgae. Agronomy Research, 7(1), 33-38.

Najdenski, H.M., Gigova, L.G., Ivan, I.I., Pilarski P.S., Lukavsky J., Tsvetkova, I.V., Ninova M.S., &

Kussovski, V.K. (2013). Antibacterial and antifungal activities of selected microalgae and cyanobacteria. International Journal of Food Science and Technology, 48, 1533-1540. https://dx.doi.org/10.1111/ijfs.12122

Noaman, N.H., Fattah, A., Khaleafa, M., & Zaky, S.H. (2004). Factors affecting antimicrobial activity of Synechococcus leopoliensis. Microbiological Research, 159, 395-402. https://dx.doi.org/10.1016/j.micres.2004.09.001

Ozdemir, G., Ulku Karabay, N., Dalay, M. C., & Pazarbasi, B. (2004). Antibacterial Activity of Volatile Component and Various Extracts of Spirulina platensis. Phytotherapy Research, 18, 754-757. https://dx.doi.org/10.1002/ptr.1541

Ördög, V., Stirk, W.A., Lenobel, R., Bancirova, M., Strand, M., & Van Standen, J. (2004). Screening microalgae for some potentially useful agricultural and pharmaceutical secondary metabolites. Journal of Applied Phycology, 16, 309-314. https://doi.org/10.1023/B:JAPH.0000047789.34883 .aa

Ozturk, S., Aslim, B., & Beyatli, Y. (2006). Biological screening of microalgae isolated from different freshwaters of Turkey: Antimicrobial activity, viability and brine shrimp lethality. Fresenius Environmental Bulletin, 15(10), 1232-1237.

Prieto, M.A., Rodríguez-Amado, I., Vazquez, J.A., & Murado, M.A. (2012). β-Carotene Assay Revisited. Application to Characterize and Quantify Antioxidant and Prooxidant Activities in a Microplate. Journal of Agricultural and Food Chemistry, 60, 8983-8993. https://dx.doi.org/10.1021/jf302218g

Radhika, D., Veerabahu, C., & Priya, R. (2012). Antibacterial activity of some selected seaweeds from the Gulf of Mannar Coast, South India. Asian Journal of Pharmaceutical and Clinical Research, 5(4), 89-90.

Rios, L. F., Klein, B. C., Luz, L. F., Maciel Filho, R., & Maciel, M. W. (2015). Nitrogen starvation for lipid accumulation in the microalga species Desmodesmus spp. Applied biochemistry and biotechnology, 175(1), 469-476. https://dx.doi.org/10.1007/s12010-014- 1283-6

Rudic, V., & Dudnicenco, T. (2000). Process for cultivation of green alga Haeamatococcus pluvialis (Flotow), MD Patent, Nr. a 2000 0154. Salem, O.M.A., Hoballah, E.M., Ghazi, S.M., & Hanna,

S.N. (2014). Antimicrobial activity of microalgal extracts with special emphasize on Nostoc sp. Life Science Journal, 11(12), 752-758.

Safafar, H., Van Wagenen, J., Møller, P., & Jacobsen, C. (2015). Carotenoids, phenolic compounds and tocopherols contribute to the antioxidative properties of some microalgae species grown on industrial wastewater. Marine drugs, 13(12), 7339-7356. https://dx.doi.org/10.3390/md13127069

Sanmukh, S., Bruno, B., Ramakrishnan, U., Khairnar, K., & Swaminathan, S. (2014). Bioactive compounds derived from microalgae showing antimicrobial activities. Journal of Aquaculture Research and Development, 5(3), 224. http://dx.doi.org/10.4172/2155-9546.1000224

Shoup, S., & Lewis, L. A. (2003). Polyphyletic origin of parallel basal bodies in swimming cells of chlorophycean green algae (Chlorophyta). Journal ofPhycology, 39(4), 789-796. https://dx.doi.org/10.1046/j.1529-8817.2003.03009.x

Tüney, I., Çadirci, B.H., Unal, D., & Sukatar, A. (2006). Antimicrobial Activities of the Extracts of Marine Algae from the Coast of Urla (İzmir, Turkey). Turkish Journal of Biology, 30, 171-175.

Vanags, J., Kunga, L., Dubencovs, K., Galvanauskas, V., & Grīgs, O. (2015). Influence of Light Intensity and Temperature on Cultivation of Microalgae Desmodesmus Communis in Flasks and Laboratory- Scale Stirred Tank Photobioreactor. Latvian Journal of Physics and Technical Sciences, 52(2), 59-70. https://doi.org/10.1515/lpts-2015-0012

Velioglu, Y. S., Mazza, G., Gao, L., & Oomah, B.D. (1998). Antioxidant Activity and Total Phenolics in Selected Fruits, Vegetables, and Grain Products. Journal of Agricultural and Food Chemistry 46, 4113-4117. https://dx.doi.org/10.1021/jf9801973

Yıldırım, A., Demirel, Z., İşleten-Hoşoğlu, M., Akgün, İ. H., Hatipoğlu-Uslu, S., & Conk-Dalay, M. (2014). Carotenoid and fatty acid compositions of an indigenous Ettlia texensis isolate (Chlorophyceae) under phototrophic and mixotrophic conditions. Applied Biochemistry and Biotechnology, 172, 1307- 1319. https://dx.doi.org/10.1007/s12010-013-0599-y

Zhang, D., & Hamauzu, Y. (2004). Phenolics, ascorbic acid, carotenoids and antioxidant activity of broccoli and their changes during conventional and microwave cooking. Food Chemistry, 88(4), 503-509. https://doi.org/10.1016/j.foodchem.2004.01.065

Zubia, M., Robledo, D., & Freile-Pelegrin, Y. (2007). Antioxidant activities in tropical marine macroalgae from the Yucatan Peninsula, Mexico. Journal of applied phycology, 19(5), 449-458. https://dx.doi.org/10.1007/s10811-006-9152-5