Cytogenetic analysis of Garra variabilis (Heckel, 1843) (Pisces, cyprinidae) from Savur stream (Mardin), Turkey

Bu çalışmada Savur Irmağı'nda (Mardin) yaşayan Garra variabilis (Helkel, 1843) bireylerinin sitogenetik analizi yapılmıştır. G. variabilis’in karyolojik analizi G-bantlama, C-bantlama, Ag-NOR boyama ve Q-bantlama teknikleri kullanılarak belirlenmiştir. Örneklerin diploit kromozom sayısı 2n=102 olarak tanımlanmıştır. Bununla birlikte G. variabilis bireylerinde iki farklı karyotip görülmüştür: dişi 42m+18sm+24st+18a (FN=186), erkekler 41m+18sm+24st+19a (FN=185). Çekirdekçik yapıcı bölgeler (NORs) gümüş boyama tekniği ile belirlenmiştir. Birçok kromozom üzerinde NOR bölgeleri görülmüştür. En büyük kromozom submetasentrik olarak tanımlanmıştır. G. variabilis'in ayrıntılı karyotipi bu çalışma ile ilk kez belirlenmiştir.

Anahtar Kelimeler:

Türkiye, karyotip, Mardin, Cyprinidae, sitogenetik

Savur Irmağı'nda (Mardin) yaşayan Garra variabilis (Helkel, 1843) (Pisces, cyprinidae)'in sitogenetik analizi, Türkiye

In this study cytogenetic analysis were carried out on Garra variabilis (Heckel, 1843) individuals from Savur stream (Mardin). The karyotype of G. variabilis was analyzed using G-banding, C-banding, Ag-NOR staining and Q-banding techniques. Diploid chromosomes number of specimens was determined as 2n=102. However, two karyotype formulae were identified in the G. variabilis specimens: female 42m+18sm+24st+18a (FN = 186) and males 41m+18sm+24t+19a (FN = 185). Nucleolar organizer regions (NORs) were identified by silver nitrate staining. NOR regions were observed on many chromosome pairs. The largest chromosome pair was defined as submetacentric. The detail karyotype of G. variabilis is reported for the first time.

Keywords:

Turkey, karyotypes, Mardin, Cyprinidae, cytogenetics,

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