Edward Ebo ONUMAH, Stephan WESSELS, Nina WILDENHAYN, Bernhard BRUMMER, Gabriele HORSTGEN SCHWARK

Stocking density and photoperiod manipulation in relation to estradiol profile to enhance spawning activity in female Nile tilapia

Bu çalışma, F1 klon çaprazlama yöntemi kullanılarak Nil tilapiyasında yumurtlama aktivitesini güçlendirmek amacıyla plazma estradiol -17β ($E _2$) seviyesine ilişkin olarak stoklama yoğunluğunun ve fotoperiyot değişimlerinin etkilerini incelemiştir. Balıklar, deney ve kontrol grubuna ayrılmış, yumurtlama yoğunluğu ve fotoperiod işlemi kombinasyonuna maruz bırakılmıştır (40 kg/$m^ 3$;6L:18D, 40 kg/$m^ 3$;12L:12D ve sırasıyla 12L:12D; 14 kg/$m^ 3$;12L:12D’de ayrı bölümlere transfer edilmiştir). Estradiyol profil analizi için hem deney hem de kontrol grubu balıklardan kaudal yüzgeç bölgesinden kan örnekleri alınmıştır. Sonuçlara göre deney grubu balıklarının günlük olarak daha fazla sayıda yumurtladıkları, toplam ve relatif fekonditenin, kuluçka çıkışının ve yüzme oranının (P

Anahtar Kelimeler:

kan kimyası, yerleşim sıklığı, döllenme gücü, östradiol, Oreochromis niloticus, yumurta bırakmak, üreme, fotoperiyodizm

Dişi Nil tilapyasında yumurtlama aktivitesini güçlendirmek Amacıyla estradiol yapısına ilişkin olarak stoklama yoğunluğu ve fotoperiot uygulaması değişimleri

This study investigated the effects of stocking density and photoperiod manipulation in relation to plasma estradiol-17β ($E _2$) profile to enhance spawning activity in female Nile tilapia using F1 clonal crosses. The fish were divided into experimental and control groups and subjected to a combination of stocking density and photoperiod treatments (40 kg/$m^ 3$; 6L:18 D, 40 kg/$m^ 3$; 12 L:12 D and transferred into single compartments at 12 L:12 D; 14 kg/$m^ 3$; 12L:12D, respectively). Blood samples were taken by caudal puncture from both experimental and control fish for estradiol profile analysis. Results showed that experimental fish exhibited significantly higher number of spawns per day, total and relative fecundity, hatching and swim-up rates (P<0.05). Regression analyses revealed a significant positive relationship between fish size (body weight), and total and relative fecundity (P<0.001). It was also revealed that $E _2$ levels demonstrated a pattern based on completed reproductive cycle in the experimental fish. The study therefore established that a combination of stocking density and photoperiod treatments can be adopted to manipulate the timing of spawning activity in female Nile tilapia without having adverse effect on other reproductive parameters such as egg qualities and fecundity. Findings further suggested that the effects of exogenous factors on manipulation of spawning activities of female Nile tilapia may be achieved as a result of hormonal changes including $E _2$ levels.

Keywords:

blood chemistry, stocking density, fecundity, estradiol, Oreochromis niloticus, spawning, reproduction, photoperiodism,

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