A Preliminary Study on Protease Activity of Russian Sturgeon, Acipencer gueldenstaedtii Brandt and Ratzenburg, 1833, at Early Life Stages

Balık yemi endüstrisi, aday türlerin optimum rasyon ihtiyacını belirlemek için araştırmalarına devam etmektedir. Bu amaçla yapılan in vitro analiz metotları, in vivo metotlara göre daha verimli olabilir. Bu çalışma iki farklı çalışmadan oluşmaktadır; bunlardan birincisi ticari besleme protokolünün proteaz enzimlerinin değişimi üzerine olan etkisini, ikinci çalışma ise farklı protein kaynaklarının Rus mersini, A. gueldenstaedtii, jüvenillerinin erken dönem proteaz aktivitiesi üzerine olan inhibisyon etkisini belirlemeyi amaçlamıştır. İlk çalışmada, canlı yem ile besleme ve karma yeme geçiş periyodunun, sindirim sistemindeki günlük proteaz miktarını istatistiki olarak etkilediği tespit edilmiştir (P

Rus Mersin Balığının, Acipencer gueldenstaedtii Brandt ve Ratzenburg, 1833, Yaşamlarının İlk Dönemlerindeki Proteaz Aktivitesi Üzerine Bir Ön Çalışma

The fish feed industry continues to researches for optimum diet demands for candidate species culture. For this purpose, in vitro analyze methods may be more efficient than in vivo assays. This study includes two different stages; the first one is about the effect of commercial feeding protocol on protease enzyme alteration and the second one is about the inhibitory effects of different protein sources on early life proteases of Russian sturgeon, A. gueldenstaedtii, juveniles. In the first step, feeding with live prey and transition period to artificial feed significantly affected the daily amount of protease in digestive system (P

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  • Alarcon, F. J., Moyano, F. J., Diaz, M., Fernandez-Diaz, C., Yufera, M., 1999. Optimization of the protein fraction of microcapsules used in feeding of marine fish larvae using in vitro digestibility techniques. Aquaculture Nutrition, 2095.1999.00093.x doi: 10.1046/j.1365
  • Alptekin, C., Gökçek, K., 2016. Balık Yemlerinde Kullanılan Farklı Protein Kaynaklarının Sibirya Mersini (Acipencer baerii Brandt 1869) Jüvenillerinin Proteaz Aktivitesi Üzerine Etkileri. Yunus Araştırma Bülteni, doi:10.17693/yunus.32866
  • Babaei, S. S., Kenari, A. A., Nazari, R., Gisbert, E., 2011. Developmental changes of digestive enzymes in Persian Sturgeon (Acipencer percius) during larval ontogeny. doi:10.1016/j.aquaculture.2011.04.032 318: 138-144.
  • Bradford, M. M. 1976. A rapid sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dry binding. Anal. Biochem., 72, 248-254.
  • Cahu, C. L. and Zambonino, J. L., 1995. Maturation of the pancreatic and intestinal digestive functions in sea bass (Dicentrarchus labrax): effect of weaning with different protein sources. Fish Physiol. Biochem., 14: 431-437.
  • Cho, C.Y. 1987. La energia en la nutricion de los peces. In: Nutricion en Acuicultura, vol. II (ed. By de la Monteros Espinosa nad Lanarta U.), pp. 197-244. CAICYT, Madrid, Spain.
  • Dimes, L. E., Haard, N. F., Dong, F. M., Rasco, B. A., Forster, J. P., Fairgrieve, W. T., Arndt, R., Hardy, R. W., Barrows, F. T., Higs, D. A., 1994. Estimation of protein digestibility. II. In vitro assay of protein in salmonid feeds. Comp. Biochem. Physiol. 108A: 363- 370. doi:10.1016/0300-9629(94)90107-4
  • Ezquerra, J. M., 1997. Enzyme technology in aquaculture: in vitro evaluation of enzymatic digestibility of dietary proteins by pH-stat for the optimization of diets for cultured Penaeus vannamei. Ph.D Thesis, CIBNOR, La Paz, Mexico.
  • Faulk, C. K., Benninghoff, A. D., Holt, G. J., 2007. Ontogeny of the gastrointestinal tract and selected digestive enzyme in cobia Rachycentron canadum (L.). J. Fish Biol. 70:567-583. doi: 10.1111/j.1095- 8649.2007.01330.x
  • Furne, M., Hidalgo, M. C., Lopez, A., Garcia- Gallego, M., Morales, A. E., Domezain, A., Domezaine, J., Sanz, A., 2005. Digestive enzyme activities in Adriatic sturgeon Acipencer naccarii and rainbow trout Oncorhynchus Aquaculture, doi:10.1016/j.aquaculture.2005.05.017 study. 391-398.
  • Garcia-Carreno, F. L., 1996. Proteinase inhibitors. Trends Food Sci. Technol., 7: 197-204. doi:10.1016/0924- 2244(96)10023-6
  • Gawlicka, A., Herold, M. A., Barrows, F. T., de le Noue, J., Hung, S. S. O., 2002. Effect of dieatary lipids on growth, fatty acid composition, intestinal absorbtion and hepatic storage in white sturgeon (Acipencer transmontanus) larvae. J. Appl. Icthyol., 18: 673-681. doi: 10.1046/j.1439-0426.2002.00371.x
  • Grabner, M., 1985. An in vitro method for measuring protein digestibility of fish feed components. Aquaculture, 8486(85)90097-3 doi:10.1016/0044
  • Hazman, B., Gökçek, K., 2014. The Effect of Different First Feeds on Proteolitic Activity of the Northern Pike, Esox lucius Linneaus 1758, Post-Larvae. Turkish Journal of Fisheries and Aquatic Sciences 14 (4): 875-878. doi: 10.4194/1303-2712-v14_4_04
  • Halver, J.E., Hardy, R.W., 2002. Fish Nutrition. Academic Press, USA. pp. 417-427.
  • Hofer, K., Köck, G. 1989. Method for quantitative determination of digestive enzymes in fish larvae. Polish Archives of Hidrobiology, 36, 439-441.
  • Kim, B. G., Divakaran, S., Brown, C. L., Ostrowski, A. C., 2001. Comparative digestive enzyme ontogeny in two larval fishes: Pacific threadfin (Polydatilus sexfilis) and bluefin trevally (Caranx melampygus). Fish Physiol. Biochem. 24: 225-241.
  • Kolkovski, S., 2001. Digestive enzymes in fish larvae and juveniles- implications and applications to formulated diets. Aquaculture 200: 181-201.
  • Lan, C. C. and Pan, B. S., 1993. In vitro digestibility sitimulating the proteolysis of feed protein in the midgut gland of grass shrimp Penaeus monodon. Aquaculture, 8486(93)90486-I doi:10.1016/0044
  • Moyao, F. J., Diaz, M., Alarcon, F. J., Saraquete, M. C., 1996. Characterization of digestive enzyme activity during larval development of gilthead seabream (Sparus auraus). Fish Pyhsiol. Biochem., 15: 121- 130.
  • Napora- Rutkowski, L., Kamaszewski, M., Bielawski, W., Ostaszewska, T., Wegner, A., 2009. Effects of starter diets on pancreatic enzyme activity in juvenile starlet (Acipencer ruthenus). The. Isr. Jour. Aqua.- Bamidgeh, 61 (2): 143-150.
  • Noori, F., Gilbert, V. S., Sorgeloos, P. 2012. Preliminary study on the activity of protease enzymes in Persian sturgeon (Acipencer persicus Borodin, 1897) larvae in response to different diets: effects on growth and survival. doi: 10.1111/j.1365-2109.2011.02816.x 43, 198-207.
  • Sanz, A., Llorente, J. L., Furne, M., Ostos-Garrido, V., Carmona, R., Domezain, A., Hidalgo, M. C., 2011. Digestive enzymes during ontogeny of the sturgeon Acipencer development. J. Appl. Ichthyol., 27: 1139-1146. doi: 10.1111/j.1439-0426.2011.01864.x pancreas
  • Segner, H., Rösch, R., Verreth, J., Win, U., 1993. Larval nutrition physiology: studies with Clarias gariepinus, Coregonus lavaretus and Scophthalmus maximus. J. World Aqua. Soc. 24: 121-134. doi: 10.1111/j.1749- 7345.1993.tb00001.x
  • Töre, Y., Gökçek, K., Alptekin, C., Szabo, T. ve Urbanyi, B., 2014. Turna, Esox lucius Linneaus 1758, Balığı Larvalarının Proteaz Aktivitesi Üzerine Ticari Yem Hammaddelerinin İnhibisyon Etkilerinin Tespiti. Doğu Anadolu 5. Su Ürünleri Sempozyumu. 31 Mayıs-2 Haziran 2014, Elazığ.
  • Walter, H.E. 1984. Proteinases: methods with hemoglobin, casein and azocoll as sub-strates, In: Methods of Enzymatic Analysis (Bergmeyer, H.J. ed.), 5, 270- 277.
  • Willot, P., Sabeau, L., Gessner, J., Arlati, G., Bronzi, P., Gulyas, T., Berni, P., 2001. Sturgeon farming in Western perspectives. Aquat. Living Resourc., 14: 367-374. doi:10.1016/S0990-7440(01)01136-6 and
  • Zambonino Infante, J.L., Cahu, C.L. 2001. Ontogeny of the gastrointestinal tract of marine fish larvae. Comp. Biochem. doi:10.1016/S1532-0456(01)00274-5 C, 130: 477-487.
Turkish Journal of Fisheries and Aquatic Sciences-Cover
  • ISSN: 1303-2712
  • Başlangıç: 2015
  • Yayıncı: Su Ürünleri Merkez Araştırma Enstitüsü - Trabzon
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