HATAYİ ZENGİN, Ökkeş YILMAZ, ZEHRA GÖKÇE, ERSİN DEMİR

Gökkuşağı Alabalığı Oncorhynchus mykiss (Walbaum 1792)’in Keseli Larvalarında Antioksidan Enzim Aktiviteleri ve Bazı Biyokimyasal Değişimler

Bu çalışmada ticari olarak yetiştirilen gökkuşağı alabalığı Oncorhynchus mykiss (Walbaum 1792)'in keseli yavrularında, yumurtadan çıktıktan sonraki 19 gün boyunca antioksidan savunma ve biyokimyasal durum çalışıldı. Çalışılan parametreler: süperoksit dismutaz (SOD), katalaz (CAT), glutatyon peroksidaz (GSHpx), glutatyon redüktaz (GR) ve glutatyon-s-transferaz (GST)'dır. Oksidatif hasar malondialdehit (MDA) oluşumu ile ölçüldü. Sonuçlarımız yumurtaların açıldığı günden itibaren katalitik aktivitenin mevcut olduğunu ve larvalar endojen besini kullanırken, 12 ve 19 günlük keseli larvalarda bulunan enzimlerde önemli değişimlerin olduğunu gösterdi. Çalışmamız yumurtaların açılmasından sonraki 12. ve 19. günlerde katalaz aktivitesinde hızlı bir artma, aynı periyotta süperoksit dismutaz aktivitesinde ise hızlı bir azalma gösterdi. Glutatyon peroksidaz 19 günlük çalışma süresi boyunca düzenli bir şekilde azaldı. Glutatyon redüktaz 12 ve 19 günlük keseli larvalarda önemli bir artış gösterdi. Keseli safha boyunca glutatyon-s-transferaz aktivitesinde önemli bir değişim olmadı. 19. günde yumurta kesesinin emilimiyle total protein içeriği hızla düştü. 12 ve 19 günlük keseli larvalardaki PUFA içeriği, diğer keseli safhalardan yüksek bulundu

Antioxidant Enzyme Activities and Some Biochemical Changes in Rainbow Trout Oncorhynchus mykiss (Walbaum 1792) Yolk-Sac Larvae

In this work we studied the biochemical status of antioxidant defences of yolk-sac larvae from the commercial fish, rainbow trout Oncorhynchus mykiss (Walbaum 1792) over a period of 19 days from hatching. The parameters studied were: superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSHpx), glutathione reductase (GR) and glutathione Stransferase (GST). Oxidative damage was measured by the formation of malondialdehyde (MDA). Our results showed that the presence of catalytic activities was observed from hatching day and significant changes in the enzymes were seen in the 12 and 19 days yolk-sac larvae, when the larvae finished their endogenous feeding. Our study indicates that catalase activity sharply increased in the 12 and 19 days post-hatch but superoxide dismutase activity sharply decreased in the same period. Glutathione peroxidase regularly decreased with age throughout the 19-day study period. Glutathione reductase showed a significant increase in the 12 and 19 days yolk-sac larvae. There were no significant changes in glutathione S-transferase activity throughout the yolk-sac stage. When the egg yolk-sac was reabsorbed, the total protein content sharply decreased at day 19. The PUFA contents in the 12 and 19 days yolk-sac larvae were found to be higher than in the other yolk-sac stage

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