Gökhan CORAL

9195

The Isolation and Characterization of Glucoamylase Enzyme of an Aspergillus niger Natural Isolate

An extracellular glucoamylase enzyme was isolated from Aspergillus niger Z10 natural isolate in a liquid Czapek Dox medium which contained soluble starch (2%) as the sole carbon source. The molecular weight of the enzyme was found to be about 100.000 Daltons through Starch-SDS Polyacrylamide Gel Electrophoresis. The optimal pH range of the enzyme was found to be 4.5-5.5 and it was determined that the thermal stability of this enzyme is quite high.

Anahtar Kelimeler:

Aspergillus niger, Glucoamylase.

The Isolation and Characterization of Glucoamylase Enzyme of an Aspergillus niger Natural Isolate

An extracellular glucoamylase enzyme was isolated from Aspergillus niger Z10 natural isolate in a liquid Czapek Dox medium which contained soluble starch (2%) as the sole carbon source. The molecular weight of the enzyme was found to be about 100.000 Daltons through Starch-SDS Polyacrylamide Gel Electrophoresis. The optimal pH range of the enzyme was found to be 4.5-5.5 and it was determined that the thermal stability of this enzyme is quite high.

Keywords:

Aspergillus niger, Glucoamylase.,

Turkish Journal of Biology-Cover

ISSN: 1300-0152
Yayın Aralığı: 6
Yayıncı: TÜBİTAK

Arşiv

Sayıdaki Diğer Makaleler

The Effects of Heavy Metals on the Pollen Germination and Pollen Tube Growth of Apples (Malus sylvestris Miller cv. Golden)

Ömer MUNZUROĞLU, Nazmi GÜR

Ratlarda desidualizasyon süresince ekstrasellüler matriksin yeniden modellenmesinde laminin ve fibronektin ile reseptör altbirimleri integrin $beta$4 ve $alpha$5' in dağılımları ve muhtemel rolleri

Ümit Ali KAYIŞLI, R. DEMİR, M. ASAR

The Seasonal Variation in Total Lipid and Fatty Acid Contents of the Gonads of Cyprinion macrostomus (HECKEL, 1843)

Bazı tahıl ve ürünlerinde okratoksin - A ve fungal kontaminasyon

Nural KARAGÖZLÜ, Mehmet KARAPINAR

The Effect of Cadmium Nitrate on Some Developmental Properties of Drosophila Melanogaster

Lütfiye GELEGEN, Elif YEŞİLADA

Işgın (Rheum ribes L.) bitkisindeki A, E ve C vitaminleri ile selenyum düzeylerinin araştırılması

Fikret KARATAŞ, Ömer MUNZUROĞLU, Nazmi Gür GÜR

Poliüretan köpük üzerine tutuklanmış Phanerochaete chrysosporium ME446' da gibberellik asit ve sitokinin üretimi

Serpil ÜNYAYAR

Çatören ve Kunduzlar (Kırka-Eskişehir) baraj göletlerindeki sazanların (Cyprinus carpio L., 1758) dokularında bor birikimi

Orta Karadeniz Bölgesi' nde avlanan istavrit (Trachurus trachurus L., 1758)' in populasyon dinamiği

Şennan YÜCEL, İbrahim ERKOYUNCU

Identification of Lactoce Plasmids in Lactococcus lastic subsp. lactis Stains

Çağla TÜKEL, Mustafa AKÇELİK