Sevil ALBAYRAK, Ahmet AKSOY, Ergin HAMZAOĞLU

Determination of Antimicrobial and Antioxidant Activities of Turkish Endemic Salvia halophila Hedge

In this study, the antioxidant activity of ethanol extract of Salvia halophila Hedge (Lamiaceae) was evaluated by 2 antioxidant assays, including phosphomolybdenum reduction and free radical scavenging activity. The ethanol extract showed free radical scavenging activity with IC50 of 67.73 mg/mL in 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. The antioxidant activity of the extract was found to be 84.87 ± 0.7 mg ascorbic acid equivalent (AAE)/g extract in phosphomolybdenum assay. In the addition to the antioxidant activity of the extract, the total phenolic content was measured. Total phenolic content of ethanol extract was found to be 8.21 ± 0.0 mg gallic acid equivalent (GAE)/g extract. Antimicrobial activity of the extract was examined against 13 bacteria and 2 yeasts by agar diffusion method. The ethanol extract of S. halophila had no effect against the tested yeasts. The ethanol extract showed antibacterial activity against all of the tested Gram (+) bacteria except Listeria monocytogenes at 1%, 2.5%, 5%, and 10% concentrations. The ethanol extract had no effect on the entire tested Gram (-) bacteria except Aeromonas hydrophila and Pseudomonas aeruginosa.

Anahtar Kelimeler:

Salvia halophila Hedge, antimicrobial and antioxidant activity, phenolic content, DPPH

Determination of Antimicrobial and Antioxidant Activities of Turkish Endemic Salvia halophila Hedge

Keywords:

Salvia halophila Hedge, antimicrobial and antioxidant activity, phenolic content, DPPH,

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Draughon FA. Use of botanicals as biopreservatives in foods. Food Technol 58(2): 20–28, 2004. 2. Essawi T, Srour M. Screening of some Palestinian medicinal plants for antibacterial activity. J Ethnopharmacol 70: 343–349, 2000. 3. Wang M, Li J, Rangarajan M et al. Antioxidative phenolic compounds from sage (Salvia officinalis). J Agr Food Chem 46: 4869–4873, 1998. 4.
Imaida K, Fukushima S, Shirui T et al. Promoting actions of
butylated hydroxy anisole and butylated hydroxy toluene on 2-stage
urinary bladder carcinogenesis and inhibition of c-glutamyl
transpeptidase- positive foci development in the liver of rats.
Carcinogenesis 4: 895–899, 1983. 5.
Madhavi DL, Salunkhe DK. Food antioxidants. In. Madhavi DL,
Bayrak A, Akgül A. Composition of essential oils from Turkish Salvia species. Phytochemistry 26: 846-847, 1987.
Bozan B, Ozturk N, Kosar M et al. Antioxidant and Free Radical Scavenging Activities of Eight Salvia species. Chem Nat Compd 38(2): 198-200, 2002.
Akkol EK, Göger F, Koşar M, Başer KHC. Phenolic composition and biological activities of Salvia halophila and Salvia virgata from Turkey. Food Chem 108: 942–949, 2008.
Kamatou GPP, Viljoen AM, Gono-Bwalya AB et al. The in vitro pharmacological activities and a chemical investigation of three South African Salvia species. J Ethnopharmacol 102: 382–390, 2005.
Tepe B, Eminagaoglu O, Akpulat HA et al. Antioxidant potentials and rosmarinic acid levels of the methanolic extracts of Salvia verticillata (L.) subsp. verticillata and S. verticillata (L.) subsp. amasiaca (Freyn & Bornm.) Bornm. Food Chem 100: 985–989, 2007.
Singleton VL, Rossi JAJr. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. American Journal of Enology and Viticulture 16: 144-158, 1965.
Prieto P, Pineda M, Aguilar M. Spectrofotometric quantitation of antioxidant capacity through the formation of a Phosphomolybdenum Complex: specific application to the determination of vitamin E. Analytical Biochemistry 269: 337-341, 1999.
Lee SK, Mbwambo, ZH, Chung HS et al. Evaluation of the antioxidant potential of natural products. Combinatorial Chemistry and High Throughput Screening 1: 35-46, 1998.
Sagdic O. Sensitivity of four pathogenic bacteria to Turkish thyme and oregano hydrosols. Food Science and Technology -LWT 36: 467-473, 2003.
Sagdic O, Aksoy A, Ozkan G. Evaluation of the antibacterial and antioxidant potentials of gilaburu (Viburnum opulus L.) fruit extract. Acta Alimentaria 35 (4): 487-492, 2006.
Kahkonen MP, Hopia AI, Vuorela, HJ et al. Antioxidant activity of plant extract containing phenolic compounds. Journal of Agricultural and Food Chem 47: 3954–3962, 1999.
Koşar M, Dorman HJD, Hiltunen R. Effect of an acid treatment on the phytochemical and antioxidant characteristics of extracts from selected Lamiaceae species. Food Chemistry 91: 525–533, 2005.
Cuvelier ME, Richard H, Berset C. Antioxidative activity and phenolic composition of pilot-plant and commercial extracts of sage and rosemary. J Am Oil Chem Soc 73: 645-652, 1996.
Satue-Gracia MT, Heinonen M, Frankel EN. Anthocyanins as antioxidants on human low-density lipoprotein and lecithin liposome systems. J Agr Food Chem 45: 3362–3367, 1997.
Negi PS, Jayaprakasha GK, Jena BS. Antioxidant and antimutagenic activities of pomegranate peel extracts. Food Chem 80: 393–397, 2003.
Niki E. Antioxidants in relation to lipid peroxidation. Chem Phy Lipids 44: 227–253, 1987.
Tepe B, Donmez E, Unlu M et al., Antimicrobial and antioxidative activities of the essential oils and methanol extracts of Salvia cryptantha (Montbret et Aucher ex Benth.) and Salvia multicaulis (Vahl). Food Chem 84: 519–525, 2004.
Tepe B, Daferera D, Sokmen A et al. Antimicrobial and antioxidant activities of the essential oil and various extracts of Salvia tomentosa Miller (Lamiaceae). Food Chem 90: 333–340, 2005.
Tepe B, Sokmen M, Akpulat H. A et al. Screening of the antioxidant potentials of six Salvia species from Turkey. Food Chem 95: 200– 204, 2006.
Lu YR, Foo LY. Antioxidant activities of polyphenols from sage (Salvia officinalis). Food Chem 75: 197–202, 2001.
Zhao GR, Xiang ZJ, Ye TX et al. Antioxidant activities of Salvia miltiorrhiza and Panax notoginseng. Food Chem 99: 767–774, 2006.
Haznedaroglu MZ, Karabay NU, Zeybeka U. Antibacterial activity of Salvia tomentosa essential oil. Fitoterapia 72: 829-831, 2001.
Delamare APL, Moschen-Pistorello IT, Artico L et al. Antibacterial activity of the essential oils of Salvia officinalis L. and Salvia triloba L. cultivated in South Brazil. Food Chem 100: 603–608, 2007.
Kabouche A, Boutaghane N, Kabouche Z et al. Components and antibacterial activity of the roots of Salvia jaminiana. Fitoterapia 76: 450–452, 2005.
Kamatou GPP, Viljoen AM, van Vuuren SF et al. In vitro evidence of antimicrobial synergy between Salvia chamelaeagnea and Leonotis leonurus. S Afr J Bot 72: 634–636, 2006.