Chitinase Production by Serratia marcescens GG5
Swollen chitin, flake chitin, powder chitin, and mushroom paste were used as substrates for chitinase production by Serratia marcescens GG5 in submerged fermentation. Enzyme production was 0.3 U/ml when the organism was grown in M9 medium supplemented with 0.5% swollen chitin and 0.5% soluble starch. Scanning electron microscopy revealed that Serratia marcescens GG5 digested the chitin flakes by producing chitinase.
Chitinase Production by Serratia marcescens GG5
Swollen chitin, flake chitin, powder chitin, and mushroom paste were used as substrates for chitinase production by Serratia marcescens GG5 in submerged fermentation. Enzyme production was 0.3 U/ml when the organism was grown in M9 medium supplemented with 0.5% swollen chitin and 0.5% soluble starch. Scanning electron microscopy revealed that Serratia marcescens GG5 digested the chitin flakes by producing chitinase.
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- 1. Dahiya N, Tewari R, Tiwari R et al. Production of an antifungal chitinase from Enterobacter sp. NRG4 and its application in protoplast production. World J Microbiol Biotechnol 21: 1611- 1616, 2005.
- 2. Bhushan B, Hoondal GS. Effect of fungicides, insecticides and allosamidin on a thermostable chitinase from Bacillus sp. BG-11. World J Microbiol Biotechnol 15: 403-404, 1999.
- 3. Chang WT, Chen CS, Wang SL. An antifungal chitinase produced by Bacillus cereus with shrimp and crab shell powder as carbon source. Curr Microbiol 47: 102-108, 2003.
- 4. Singh G, Dahiya N, Hoondal GS. Optimization of chitinase production by Serratia marcescens GG5. Asian J Microbiol Biotechnol Env Sci. 7: 383-385, 2005.
- 5. Mukherjee G, Sen SK. Characterization and identification of chitinase producing Streptomyces venezuelae P10. Ind J Exp Biol 42: 541-544, 2004.
- 6. Monreal J, Reese ET. The chitinase of Serratia marcescens. Canadian J Microbiol 15: 689-696, 1969.
- 7. Reissig JL, Strominger JL, Leloir LF. A modified colorimetric method for the estimation of N-Acetylamino sugars. J Biol Chem 217: 959-966, 1959.
- 8. Lowry OH, Rosebrough NJ, Farr AC et al. Protein measurement with Folin Phenol reagent. J Biol Chem. 193: 265-275, 1951.
- 9. Joo GJ. Purification and characterization of an extracellular chitinase from the antifungal biocontrol agent Streptomyces halstedii Biotechnol Lett. 27: 1483-1486, 2005.
- 10. Mahadevan B, Crawford DL. Properties of the antifungal biocontrol agent Streptomyces lydicus WYEC 108. Enzyme Microb Tech 20: 489-493, 1997.
- 11. Xia G, Jin C, Zhou J et al. A novel chitinase having a unique mode of action from Aspergillus fumigatus YJ-407. Eur J Biochem 268: 4079-4085, 2001.
- 12. Andronopoulon E, Vorgias CE. Multiple components and induction mechanism of the chitinolytic system of the hyperthermophilic archaeon Thermococcus chitonophagus. Applied Microbial and Biotech 65: 694-702, 2004.
- 13. Vionis AP, Niemeyer F, Karagouni AD. Production and processing of a 59 kDa exochitinase during growth of Streptomyces lividans carrying pCHIO12 in soil microcosms amended with crab or fungal chitin. Appl Environ Microbiol 62: 1774-1780, 1996.