In vivo Anti-Ulcerogenic Activity of Equisetum telmateia Ehrh. Extracts Used in Turkish Folk Medicine

The aerial part of Equisetum telmateia Ehrh. is used against various conditions including stomachache and peptic ulcer in Turkish folk medicine. In order to prove the claimed anti-ulcerogenic potential of the plant, aqueous and methanol extracts were prepared from aerial parts of E. telmateia and investigated for their anti-ulcerogenic effect on ethanol-induced gastric ulcer model in rats. Pharmacological experiments clearly demonstrated that the oral administration of both methanol and aqueous extracts of Equisetum telmateia Ehrh. plant showed significant stomach protection (77.9% and 100% ulcer inhibition, respectively) against the applied model of ulcerogenesis (P < 0.001). Since both extracts were found significantly active, 80% ethanol was preferred for the extraction of the plant material and this crude methanolic extract was further fractionated by successive solvent extractions with chloroform and H2O saturated n-BuOH to obtain the chloroform and n-BuOH extracts as well as the remaining aqueous extract. The 80% ethanol extract and the solvent extracts were then submitted to pharmacological assay using the same in vivo experimental ulcer model. All the extracts statistically showed potent anti-ulcerogenic effect on this model (P < 0.001). However, the remaining aqueous extract was found prominent (98.2% ulcer inhibition). Consequently, folkloric utilization of E. telmateia on peptic ulcer has been confirmed in the present study by using ethanol-induced experimental ulcer model in rats.

Anahtar Kelimeler:

Equisetum telmateia, Equisetaceae, Horsetail, Anti-ulcer activity, Ethanol-induced ulcerogenesis, Peptic ulcer

In vivo Anti-Ulcerogenic Activity of Equisetum telmateia Ehrh. Extracts Used in Turkish Folk Medicine

Keywords:

Equisetum telmateia, Equisetaceae, Horsetail, Anti-ulcer activity, Ethanol-induced ulcerogenesis, Peptic ulcer,

PDF

___

Yeşilada E, Gürbüz I. A compilation of the studies on the anti- ulcerogenic effects of medicinal plants. In: Singh S, Singh VK, and Govil JN. ed. Recent Progress in Medicinal Plants. Vol.II. Phytochemistry and Pharmacology. SCI Tech Publishing LLC; 2003: pp. 111-174.
Baytop T. Therapy with medicinal plants in Turkey, past and present. Nobel Tıp Kitapevi. Istanbul; 1999.
Gürbüz İ, Akyüz Ç, Yeşilada E et al. Anti-ulcerogenic effect of Momordica charantia L. fruits on various ulcer models in rats. J Ethnopharmacol 71: 77-82, 2000.
Gürbüz İ, Üstün O, Yeşilada E et al. In vivo gastroprotective effects of five Turkish folk remedies against ethanol-induced lesions. J Ethnopharmacol 83: 241-244, 2002.
Gürbüz İ, Üstün O, Yeşilada E et al. Anti-Ulcerogenic activity of some plants used as folk remedy in Turkey. J Ethnopharmacol 88: 93-97, 2003.
Gürbüz İ, Özkan AM, Yeşilada E et al. Anti-Ulcerogenic activity of some plants used in folk medicine of Pınarbaşı (Kayseri-Turkey). J Ethnopharmacol 101: 313-318, 2005.
Gürbüz İ, Yeşilada E. Evaluation of the anti-ulcerogenic effect of sesquiterpene lactones from Centaurea solstitialis L. ssp. solstitialis by using various in vivo and biochemical techniques. J Ethnopharmacol 112: 284-291, 2007.
Yeşilada E, Gürbüz İ, Shibata H. Screening of Turkish anti- ulcerogenic folk remedies for anti-Helicobacter pylori activity. J Ethnopharmacol 66: 289-93, 1999.
Yeşilada E, Gürbüz İ, Bedir E et al. Isolation of anti-ulcerogenic sesquiterpene lactones from Centaurea solstitialis L. ssp. solstitialis through bioassay-guided fractionation procedures in rats. J Ethnopharmacol 95: 213-219, 2004.
Yeşilada E, Gürbüz İ. Evaluation of the anti-ulcerogenic effect of the flowering herbs of Hypericum perforatum. J Fac Pharm Gazi 15: 77-83, 1998.
Cullen J. Pteridophyta. In: Davis PH. ed. Flora of Turkey and The East Aegean Islands. Vol 1. Edinburg University Press; 1997: pp. 31-34.
Akalın E, Alpınar K. An investigation on medicinal and edible wild plants of Tekirdağ. Ege Üniv Ecz Fak Der 2: 1-11, 1994.
Ertuğ F. Bodrum yöresinde halk tıbbında yararlanılan bitkiler. In: Başer KHC and Kırımer N. ed. 14. Bitkisel İlaç Hammaddeleri Toplantısı; 2004.
Ezer N, Arısan ÖM. Folk medicines in Merzifon (Amasya, Turkey). Turk J Bot 30: 223-230, 2006.
Gülay EG, Özhatay N. An ethnobotanical study in Çatalca (European part of Istanbul) II. Turkish J Pharm Sci 3: 73-89, 2006.
Koçyiğit M, Özhatay N. Wild plants used as medicinal purpose in Yalova (Northwest Turkey). Turkish J Pharm Sci 3: 91-103, 2006.
Kültür Ş. Medicinal plants used in Kırklareli Province (Turkey). J Ethnopharmacol 111: 341-364, 2007.
Tuzlacı E, Aymaz PE. Turkish folk medicinal plants, Part IV: Gönen (Balıkesir). Fitoterapia 72: 323-343, 2001.
Tuzlacı E, Tolon E. Turkish folk medicinal plants, Part III: Şile (İstanbul). Fitoterapia 71: 673-685, 2000.
Uğurlu E, Seçmen Ö. Medicinal plants popularly used in the villages of Yunt Mountain (Manisa-Turkey). Fitoterapia 79: 126- 131, 2007.
Uzun E, Sarıyar G, Adsersen et al. Traditional medicine in Sakarya province (Turkey) and antimicrobial activities of selected species. J Ethnopharmacol 95: 287-296, 2004.
Yeşilada E, Sezik E, Honda G et al. Traditional medicine in Turkey IX. Folk medicine in north-west Anatolia. J Ethnopharmacol 64: 195-210, 1999.
Yazıcıoğlu A, Tuzlacı E. Folk medicinal plants of Trabzon (Turkey). J Pharm Univ Mar 11: 333-342, 1995.
Robert A, Nezamis JE, Lancester C et al. Cytoprotection by prostaglandins in rats. Gastroenterology 77: 433-43, 1979.
Yeşilada E, Gürbüz İ, Ergun E. Effects of Cistus laurifolius L. flowers on gastric and duodenal lesions. J Ethnopharmacol 55: 201-211, 1997.
Geiger H, Lang U, Britsch E et al. Flavonol glycosides from Equisetum telmateia. Phytochemistry 17: 336-337, 1978.
Baytop T, Gürkan E. Pharmacognostical investigations on some of the Equisetum species (E. palustre, E. ramosissimum, E. telmateia) of Turkey. J Fac Pharm Istanbul 8: 63-74, 1972.
Correia H, Gonzalez-Paramas A, Teresa AM et al. Characterization of polyphenols by HPLC-PAD-ESI/MS and antioxidant activity in Equisetum telmateia. Phytochem Anal 16: 380-387, 2005.
Milovanovic V, Radulovic N, Todorocic Z et al. Antioxidant, antimicrobial and genotoxicity screening of hydro-alcoholic extract of five Serbian Equisetum species. Plant Food Hum Nut 62: 113- 119, 2007.
Stajner D, Popovic BM, Canadanovic-Brunet J et al. Free radical scavenging activity of three Equisetum species from Fruska gora mountain. Fitoterapia 77: 601-604, 2006.
Szelenyi I, Brune K. Possible role of oxygen free radicals in ethanol-induced gastric mucosal damage in rats. Dig Dis Sci 33: 865-871, 1988.
Oka S, Ogino K, Hobara T et al. Effects of various mucosal protective drugs on diethyldithiocarbamate-induced antral ulcer in rats. Eur J Pharmacol 197: 99-102, 1991.