Hacer MURATOĞLU, Zihni DEMİRBAĞ, Kazım SEZEN

An entomopathogenic bacterium, Pseudomonas putida, from Leptinotarsa decemlineata

The Colorado Potato Beetle (CPB), Leptinotarsa decemlineata (Say) (Col.: Chrysomelidae), is a pre-eminent pest on solanaceous crops worldwide, and much research has focused on the different components of its variable population dynamics. L. decemlineata has developed resistance to insecticides used for its control. Consequently, alternative methods are needed urgently for the control of this pest. In this study, in order to find a more effective and safer biological control agent against L. decemlineata, we isolated a new bacterial isolate (Ld4) from L. decemlineata, and tested it for insecticidal effects on L. decemlineata larvae. Also, the insecticidal effect of Ld4 was compared with Ld3, which was isolated from L. decemlineata. According to the morphological, physiological, biochemical tests, API20E and API50CH panel test systems, and 16S rRNA sequence homologies, this isolate was identified as Pseudomonas putida. The highest insecticidal effect determined on L. decemlineata within 5 days was 100%, and this activity was exhibited by Ld4 isolate. Our results indicate that Ld4 isolate may be valuable as a biological control agent for the biological control of L. decemlineata.

Anahtar Kelimeler:

Key words: Pseudomonas putida, insecticidal activity, microbial control

An entomopathogenic bacterium, Pseudomonas putida, from Leptinotarsa decemlineata

Keywords:

insecticidal activity, microbial control, Pseudomonas putida,

PDF

___

SIS. Agricultural Structure (Production, Price, Value). Republic of Turkey. State Institute of Statistics. Ankara; 2000: pp.588.
Casagrande RA. Th e Colorado potato beetle: 125 years of mismanagement. Bull Entomol Soc America 33: 142-150, 1987.
URL-1 http://www.eppo.org/QUARANTINE/insects/Leptino- tarsa_decemlineata/ LPTNDE_ds.pdf
Bouchard E, Michaud D, Cloutier C. Molecular interactions between an insect predator and its herbivore prey on transgenic potato expressing a cysteine proteinase inhibitor from rice. Mol Ecol 12: 2429-2437, 2003.
Forgash AJ. Insecticide resistance in the Colorado potato beetle. Agri Exp Sta Res Bull 704: 33-52, 1985.
Ferro ND, Boiteau G. Management of insect pests, In: Rowe RC ed. Potato Health Management. APS Press, St. Paul; 1993: pp. 103-115.
Osborn F, Berlioz L, Vitelli-Flores J et al. Pathogenic eff ects of bacteria isolated from larvae of Hylesia metabus Crammer (Lepidoptera: Saturniidae). J Invertebr Pathol 80: 7-12, 2002.
Lau WL, Jumars PA, Ambrust EV. Genetic diversity of attached bacteria in hindgut of the deposit-feeding shrimp Neotrypaea (formerly Callianassa) californiensis (Decapoda: Th alassinidae). Micro Ecol 43: 455-466, 2002.
Th iery I, Frachon E. Identifi cation, isolation, culture and preservation of entomopathogenic bacteria. In Lacey, A.L. (ed): Manual of Techniques in Insect Pathology. pp. 55-73. Academic Press, London; 1997.
Sezen K, Demirbağ Z. Isolation and insecticidal activity of some bacteria from the hazelnut beetle (Balaninus nucum L.). Appl Entomol Zool 34: 85-89, 1999.
Sezen K, Yaman M, Demirbağ Z. Insecticidal potential of Serratia marcescens Bn10. Biologia 56: 333-336, 2001.
Demir I, Sezen K, Demirbağ Z. Th e fi rst study on bacterial fl ora and biological control agent on Anoplus roboris (Sufr., Coleoptera). J Microbiol 40: 104-108, 2002.
Katı H, Sezen K, Beldüz AO et al. Characterization of a Bacillus thuringiensis subsp. kurstaki strain isolated from Malacosoma neustria L. (Lepidoptera: Lasiocampidae). Biologia 60: 301- 305, 2005.
Sezen K, Demir I, Demirbağ Z. Identifi cation and pathogenicity of entomopathogenic bacteria from common cockchafer, Melolontha melolontha L. (Col., Scarabaeidae). New Zealand J Crop Hortic Sci 35: 79-85, 2007.
Lipa JJ, Wiland E. Bacteria isolated from cutworms and their infectivity to Agrotis sp. Acta Microbiol Pol 4: 127-140, 1972.
Krieg NR, Holt JG. Gram-negative aerobic rods and cocci, In Palleroni, N.J. (ed): Bergey’s Manual of Systematic Bacteriology. pp. 140-218. Williams and Wilkins, Baltimore; 1986.
Sneath PHA, Mair NS, Sharpe ME et al. Regular, nonsporing Gram-positive rods. In Kandler, O. and Weiss, N. (eds): Bergey’s Manual of Systematic Bacteriology. pp. 1208-1260. Williams and Wilkins, Baltimore; 1986.
Poinar GO. Identifi cation of the Groups of Insect Pathogens. Plenum Press. New York; 1978: pp. 246.
Christine LC, Ted RJ. Laboratory Experiments in Microbiology. Benjamin/Cummings. California; 1992.
Alsina M, Blanch AR. A set of keys for biochemical identication of environmental Vibrio species. J Appl Bacteriol 76: 79-85, 1994.
Behrendt U, Ulrich A, Schumann P et al. A taxonomic study of bacteria isolated from grasses: a proposed new species Pseudomonas graminis sp. nov. Int J Syst Bacteriol 49: 297-308, 1999.
Peix A, Rivas R, Mateos PF et al. Pseudomonas rhizosphaerae sp. nov., a novel species that actively solubilizes phosphate in vitro. Int J Syst Evol Microbiol 53: 2067-2072, 2003.
Sambrook J, Fritsch EF, Maniatis T. Molecular Cloning, 2nd ed. Cold Spring Harbor Laboratory Press; 1989: pp. 253.
Altschul SF, Gish W, Miller W et al. Basic local alignment search tool. J Mol Biol 215: 403-410, 1990.
Ben-Dov E, Boussiba S, Zaritsky A. Mosquito larvicidal activity of E. coli with combinations of genes from B. thuringiensis subsp. israelensis. J Bacteriol 177: 2851-2857, 1995.
Moar WJ, Pusztzai-Carey M, Mack TP. Toxity of purifi ed proteins and the HD-1 strain from Bacillus thuringiensis against lesser cornstalk borer (Lepidoptera: Pyralidae). J Eco Entomol 88: 606-609, 1995.
Sezen K, Katı H, Nalçacıoğlu R et al. Identifi cation and pathogenicity of bacteria from european shot-hole borer, Xyleborus dispar Fabricius (Coleoptera: Scolytidae), Annals of Microbiology, 58 (2), 173-179, 2008.
Minitab. User’s Guide, Release 11. Minitab. State College; PA: 1997.
Demir I, Gurel N, Nalcacıoglu R et al. Comparative susceptibilities of six insect cell lines to infection by Malacosoma neustria Nucleopolyhedrovirus (ManeNPV). Turk. J Biol 33: 259-273, 2009.
Wegensteiner R. Chytridiopsis typographi (Protozoa, Microsporidia) and other pathogens in Ips typographus (Coleoptera, Scolytidae). IOBC/wprs Bull 17(3): 39-42, 1994.
Gerner-Smidt P. Acinetobacter. Epidemiological and taxonomic aspects. AMPIS 102 (Suppl. 47): 5-41, 1994.
Grimont PDA, Bouvet PJM. Taxonomy of Acinetobacter, p.25- 36. In Towner KJ, Bergogne-Berezin E, Fewson CA (ed), Th e biology of Acinetobacter. Plenum Press, New York, 1991.
Sezen K, Demir I, Demirbağ Z. Study of the bacterial fl ora as a biological control agent of Agelastica alni L. (Coleoptera: Chrysomelidae). Biologia 59: 327-331, 2004.
Sezen K, Demir I, Katı H et al. Investigations on bacteria as a potential biological control agent of summer chafer, Amphimallon solstitiale L. (Coleoptera: Scarabaeidae). J Microbiol 43: 463-468, 2005.
Ivanov GM, Gukasjan AB. Charakteristika nekatorych stammov roda Pseudomonas vydelennych iz nasekomych, In Gukasjan AB ed. Mikroorganizmy v Borbe s Vrediteljami Lesnogo Chozjajstva, Nauka, Moskow; 1966: pp. 135.
Yılmaz H, Sezen K, Katı H et al. Isolation and Identifi cation of Bacteria Associated with European Spruce Bark Beetle, Dendroctonus micans Kugelann (Coleoptera, Scolytidae). Biologia 61: 679-686, 2006.
Bucher GE. Identifi cation of bacteria found in insects. In Burges, H.D. (ed): Microbial Control of Pests and Plant Diseases. pp. 7-33. Academic Press, New York; 1981.
Schneider M, Dorn A. Diff erential infectivity of two Pseudomonas species and the immune response in the milkweed bug, Oncopeltus fasciatus (Insecta: Hemiptera). J Invertebr Pathol 78: 135-140, 2001.
Timmis KN. Pseudomonas putida: A cosmopolitan opportunist par excellence. Environ Microbiol 4: 779-781, 2002.
Li H, Medina F, Vinson SB et al. Isolation, characterization, and molecular identifi cation of bacteria from the red imported fi re ant (Solenopsis invicta) midgut. J Inver Pathol 89: 203-209, 2005.
Vossen A, Abdulmawjood A, Lammler C et al. Identifi cation and molecular characterization of beta-hemolytic streptococci isolated from harbor seals (Phoca vitulina) and grey seals (Halichoerus grypus) of the German north and Baltic seas. J Clin Microbiol 42: 469-473, 2004.