Belgin Göçmen TAŞKIN, Vatan TAŞKIN, Köksal KÜÇÜKAKYÜZ

Electrophoretic analysis of genetic diversity in natural house fly (Musca domestica L.) populations from the western and southern coasts of Turkey

In this study genetic variation was determined with isozyme analysis in 8 natural house fly (Musca domestica L.) populations from the western and southern coasts of Turkey, a part of the southern Palearctic region. For this purpose, 7 isozyme systems (ACP, AOX, CAT, HBDH, HXK, MDH, and SOD) were resolved using the native polyacrylamide gel electrophoresis (native-PAGE) technique. A total of 9 loci and 20 alleles were detected. The proportion of polymorphic loci in populations ranged from 44.44% to 66.67%. The overall mean number of effective alleles per locus (Ne) was 1.314 ± 0.454. The mean estimated expected heterozygosity (He) of populations was 0.177 ± 0.228. A very high proportion (93.70%) of genetic diversity was due to variation within populations while a relatively small portion (6.30%) was due to variation among populations. The average level of gene flow (Nm) was calculated to be 3.7182 per generation among all populations studied. The gene flow detected among the Aegean populations (Nm = 6.4451) was 2 times higher than the gene flow detected among the Mediterranean populations (Nm = 3.3331). Our results confirmed the high levels of genetic diversity in house flies reported earlier, supporting the notion that the southern Palearctic region, particularly the Middle East, is the most likely location for the origin of M. domestica.

Anahtar Kelimeler:

Key words: Musca domestica, isozymes, genetic variation, Palearctic region, native-PAGE

Electrophoretic analysis of genetic diversity in natural house fly (Musca domestica L.) populations from the western and southern coasts of Turkey

Keywords:

Key words: Musca domestica, isozymes, genetic variation, Palearctic region, native-PAGE,

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Black WC, Krafsur ES. Electrophoretic analysis of genetic- variability in the house-fl y (Musca domestica L). Biochem Genet 23: 193-203, 1985.
Krafsur ES, Helm J, Black WC. Genetic diversity at electrophoretic loci in the house fl y, Musca domestica L. Biochem Genet 30: 317-328, 1992. 3. Marquez JG, Krafsur ES. Gene fl ow among geographically diverse housefl y populations (Musca domestica L.): a worldwide survey of mitochondrial diversity. J Hered 93: 254-259, 2002.
Krafsur ES. Age composition and seasonal phenology of house fl y (Diptera: Muscidae) populations. J Med Entomol 22: 515-523, 1985.
Black WC, Krafsur ES. Temporal and spatial trends in allozyme frequencies in housefl y populations, Musca domestica L. Th eor Appl Genet 71: 673-681, 1986.
Black WC, Krafsur ES. Seasonal breeding structure in housefl y, Musca domestica L. populations. Heredity 56: 289-298, 1986.
Skidmore P. Th e biology of the Muscidae of the world. Series Entomologica, vol. 29. Kluwer Academic Publishers Group Dordrecht, Th e Netherlands; 1985. 8. Pont AC. A review of the Fanniidae and Muscidae of the Arabian Peninsula. Fauna of Saudi Arabia 12: 312-365, 1991. 9. Krafsur ES, Bryant NL, Marquez JG et al. Genetic distances among North American, British, and West African house fl y populations (Musca domestica L.). Biochem Genet 38; 275-284, 2000.
Taskin V, Kence M. Th e genetic basis of malathion resistance in housefl y (Musca domestica L.) strains from Turkey. Russ J Genet 40; 1475-1482, 2004.
Whitmore DH. Electrophoretic and Isoelectric Focusing Techniques in Fisheries Management. CRC Press Florida USA; 1990.
Manchenko GP. Handbook of Detection of Enzymes on Electrophoretic Gels. Second Edition, CRC Press Washington, DC; 2003.
Conkle MT, Hodgskiss PD, Nunnally LB et al. Starch Gel Electrophoresis of Conifer Seeds: A Laboratory Manual. United States Department of Agriculture Forest Service. Pacific Southwest Forest and Range Experiment Station. General Technical Report;1982.
Kimura M, Crow JF. Th e number of alleles that can be maintained in a finite population. Genetics 49: 725-38, 1964.
Nei M. Analysis of gene diversity in subdivided populations. Proc Natl Acad Sci USA 70: 3321-3323, 1973.
Levene H. On a matching problem arising in genetics. Ann Math Stat 20: 91-94, 1949.
Wright S. Th e genetic structure of populations. Ann Eugen 15: 323-354, 1951.
Wright S. Evolution and the Genetics of Populations. Vol. 4. Variability Within and Among Natural Populations. Th e University of Chicago Press; 1978.
Nei M. Genetic distance between populations. Am Nat 106: 283-292, 1972.
Sneath PHA, Sokal RR. Principles of Numerical Taxonomy. 2nd Ed. Freeman, San Francisco; 1973.
Nei M. Molecular Evolutionary Genetics. Columbia University Press, New York; 1987.
Wright S. Isolation by distance. Genetics 8: 114-138, 1943.
Wright S. Evolution and the Genetics of Populations, Vol 2. Th e Th eory of Gene Frequencies. Th e University of Chicago Press; 1969.
Yeh FC, Boyle T, Rongcai Y et al. POPGENE VERSION 1.32 Microsoft Window-based Freeware for Population Genetic Analysis, 1999.
Slatkin M. Isolation by distance in equilibrium and non- equilibrium populations. Evolution 47: 264-279, 1993.
Bohanak AJ. IBD (Isolation by Distance): a program for analyses of ısolation by distance. J Hered 93: 153-154, 2002.
West LS. Th e housefl y: its natural history, medical importance and control. Ithaca, NY Comstock; 1951.
Agatsuma T, Takeuchi T. Genetic polymorphism of LDH isozymes in the house fl y, Musca domestica II. Geographic cline observed in natural populations. Japan J Genetics 53: 317-325, 1978.
Sezgin E, Duvernell DD, Matzkin LM, et al. Single-locus latitudinal clines and their relationship to temperate adaptation in metabolic genes and derived alleles in Drosophila melanogaster. Genetics 168: 923-931, 2004.
Kandemir I, Kence M, Kence A. Genetic and morphometric variation in honeybee (Apis mellifera L.) populations of Turkey. Apidologie 31: 343-356, 2000.
Richmond RC, Powell JR. Evidence of heterosis associated with an enzyme locus in a natural population of Drosophila. Proc Natl Acad Sci USA 67: 1264-1267, 1970.
Graur D. Gene diversity in Hymenoptera. Evolution 39: 190-199, 1985.