Total phenolic contents and antioxidant In, Jehad AHMED, Ayşegül GÜVENÇ, Nurgün KÜÇÜKBOYACI, Ayşe BALDEMİR, Maksut COŞKUN

Total phenolic contents and antioxidant activities of Prangos Lindl. (Umbelliferae) species growing in Konya province (Turkey)

Prangos species are widely used as medicinal plants in Turkey, and 14 species of this genus grow naturally in Anatolia. In the present study, the phenolic contents and antioxidant activities of the water and methanol (MeOH) extracts obtained from the root, herb, and fruits of 4 species of Prangos (Prangos ferulacea, P. heyniae, P. meliocarpoides var. meliocarpoides, and P. uechtritzii) collected in Konya province were compared. The phenolic contents of the samples were determined using Folin-Ciocalteu's phenol reagent. Antioxidant activities of the extracts were studied by qualitative DPPH. (1,1-diphenyl-2-picrylhydrazyl radical) assay to detect the free radical scavenging activity and by thiobarbituric acid (TBA) assay to detect their liposome lipid peroxidation. Total phenolic contents of the MeOH extracts were found to range from 77.99 to 140.29 mg/g and the water extracts ranged from 37.53 to 97.29 mg/g in dry weight expressed as gallic acid equivalents (GAE). All extracts showed a slightly antioxidant activity with the DPPH. test. High activity was observed in the MeOH extracts when compared to the water extracts in the TBA test.

Anahtar Kelimeler:

Key words: Prangos ferulacea Lindl., Prangos heyniae H. Duman & M. F. Watson., Prangos meliocarpoides Boiss. var. meliocarpoides, Prangos uechtritzii Boiss. & Hausskn., Umbelliferae, total phenolic contents, antioxidant activity

Total phenolic contents and antioxidant activities of Prangos Lindl. (Umbelliferae) species growing in Konya province (Turkey)

Keywords:

Key words: Prangos ferulacea Lindl., Prangos heyniae H. Duman & M. F. Watson., Prangos meliocarpoides Boiss. var. meliocarpoides, Prangos uechtritzii Boiss. & Hausskn., Umbelliferae, total phenolic contents, antioxidant activity,

PDF

___

Kumar PS, Sucheta S, Deepa VS et al. Antioxidant activity in selected Indian medicinal plants. African J Biotechnol 7: 1826- 1828, 2008.
Duman H. “Prangos Lindl.” in Flora of Turkey and the East Aegean Islands. Ed. Güner A, Özhatay N, Ekim T, Başer KHC. Vol 11, (Supplement 2), Edinburgh University Press, Edinburgh, p. 141-143, 2000.
Duran A, Sağıroğlu M, Duman H. Prangos turcica (Apiaceae): a new species from South Anatolia, Turkey. Ann. Bot. Fennici. 42: 67-72, 2005.
Pimenov MG, Akalın E, Kljuykov E. Prangos ilaniae (Umbelliferae), a new species from Western Turkey. Candollea 60: 379-385, 2005.
Tada Y, Shikishima Y, Takaishi Y et al. Coumarins and γ-pyrone derivatives from Prangos pabularia: antibacterial activity and inhibition of cytokine release, Phytochem 59: 649-654, 2002.
Baser KHC, Demirci B, Demirci F et al. A new bisabolene derivative from the essential oil of Prangos uechtritzii fruits. Planta Medica 66: 674-677, 2000.
Dokovic DD, Bulatovic VM, Boziç BD et al. 3,5-Nonadiyne isolated from the rhizome of Cachrys ferulacea inhibits endogenous nitric oxide release by rat peritoneal macrophages. Chem Pharm Bull 52: 853-854, 2004.
Başer KHC, Özek T, Demirci B, et al. Composition of the essential oil of Prangos heyniae H. Duman & M. F. Watson, a new endemic from Turkey. Flavour Frag J 15: 47-49, 2000.
Özcan M, Bağcı Y, Akgül A et al. Chemical composition of the essential oil of Prangos uechtritzii Boiss. Et Hausskn. fruits from Turkey. J Essent Oil Res 12: 183-185, 2000.
Tawaha K, Al-Muhtaseb S, Al-Khalil S. Coumarins from the aerial parts of Prangos ferulacea. Alex J Pharm Sci 15: 89-91, 2001.
Tsetlin AL, Vermel EM, Kuznetsova GA et al. Cytotoxic activity of phenolic and coumarin derivatives from plants of the genus Prangos. Trudy Botanicheskogo Instituta, Akademiya Nauk SSSR, Seriya 5: Rastitel’noe Syr’e, 16: 7-9, 1972.
Razavi SM, Nazemiyeh H, Delazar A et al. Coumarins from the roots of Prangos uloptera. Phytochem Lett 1: 159-162, 2008.
Harborne JB, Williams CA. Flavonoid patterns in the fruits of the umbelliferae. Phytochem 11: 1741-1750, 1972.
Kogure K, Yamauchi I, Tokumura A et al. Novel antioxidants isolated from plants of the genera Ferula, Inula, Prangos and Rheum collected in Uzbekistan. Phytomed 11: 645-651, 2004.
Mavi A, Terzi Z, Özgen U et al. Antioxidant properties of some medicinal plants: Prangos ferulacea (Apiaceae), Sedum sempervivoides (Crassulaceae), Malva neglecta (Malvaceae), Cruciata taurica (Rubiaceae), Rosa pimpinellifolia (Rosaceae), Galium verum subsp. verum (Rubiaceae), Urtica dioica (Urticaceae). Biol Pharm Bull 27: 702-705, 2004.
Slinkard K, Singleton VL. Total phenols analysis: automation and comparison with manual methods. Am J Enology Viticulture 28: 49-55, 1977.
Singleton VL, Rossi JA. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am J Enology Viticulture 16: 144-158, 1965.
Javanmardi J, Stushnoff C, Locke E et al. Antioxidant activity and total phenolic content of Iranian Ocimum accessions. Food Chem 83: 547-555, 2003.
Halliwell B, Chirica S. Lipid peroxidation: its mechanism, measurement, and signifi cance. Am J Clin Nutr 57: 715-725, 1993.