The analgesic effect of the ethanolic extract of Matricaria aurea

The analgesic effect of the ethanolic whole plant extract of Matricaria aurea L. (Asteraceae) was studied in rats using the cold water tail flick assay and in mice using the tail immersion, tail clip, acetic-acid-induced writhing, and formalin pain tests. The results showed dose-dependent and significant (P < 0.001) increases in pain threshold at 60 min post-treatment with doses of 100, 200, and 300 mg/kg of the extract in tail flick, tail immersion, and tail clip tests. The effects of the extract were significantly (P < 0.001) lower than those produced by morphine (10 mg/kg) in the same tests. The extract (100, 200, and 300 mg/kg) exhibited a dose-dependent inhibition of writhing and also showed a significant (P < 0.001) inhibition in both phases of the formalin pain test, but with a less intense effect in the first phase. The results indicate that the analgesic effect of M. aurea ethanolic extract is both centrally and peripherally mediated.

Anahtar Kelimeler:

Key words: Matricaria aurea, analgesic activity, formalin pain test, tail clip test, mouse writhing assay

The analgesic effect of the ethanolic extract of Matricaria aurea

Keywords:

Key words: Matricaria aurea, analgesic activity, formalin pain test, tail clip test, mouse writhing assay,

PDF

___

Ali-Shtayeh M, Yaniv Z, Mahajna J. Ethnobotanical survey in the Palestinian area: a classifi cation of the healing potential of medicinal plants. Journal of Ethnopharmacology 73: 221-32, 2000.
Ali-Shtayeh MS, Jamous RM, Al-Shafi e’ JH et al. Traditional knowledge of wild edible plants used in Palestine (Northern West Bank): a comparative study. Journal of Ethnobiology and Ethnomedicine 12: 4-13, 2008.
Al-Khalil S. A survey of plants used in Jordanian traditional medicine. International Journal of Pharmacognosy 33: 317- 323, 1995.
Oran S, Al-Eisawi D. Check-list of medicinal plants in Jordan. Dirasat. Medical and Biological Sciences 25: 84-112, 1998.
Pino JA, Bayat F, Marbot R et al. Essential oil of chamomile Chamomilla recutita (L.) Rausch. from Iran. Journal of Essential Oil Research 14: 407-408, 2002.
Pino JA, Marbot R, Agiiero J et al. Essential oil of chamomile Chamomilla recutita (L.) Rausch. from Cuba. Journal of Essential Oil Research 3: 1-3, 2000.
Mann C, Staba EJ. Th e chemistry, pharmacology, and commercial formulations of Chamomile. In Herbs, Spices, and Medicinal Plants: Recent Advances in Botany, Horticulture, and Pharmacology, Volume 1, Craker LE, Simon JE (eds). Oryx Press. Phoenix. 235-280, 1986.
Matos FJ, Machado MI, Alencar JW et al. Constituents of Brazilian chamomile oil. Journal of Essential Oil Research 5: 337-339, 1993.
Mimica-Dukic N, Lukic V, Pavkov R et al. Study of chemical composition and microbiological contamination of chamomile tea. Acta Horticulturae 333: 137-141, 1993.
Stanev S, Zheljazkov V, Janculoff Y. Variation of chemical compounds in the essential oil from some native forms of chamomile (Chamomilla recutita L.). In: Breeding Research in Aromatic and Medicinal Plants. Jahrgang, Heft , Ed.F. Pank. 214-217, 1996.
Ahmed A, Abou-Elela M. Highly oxygenated bisabolenes and an acetylene from Matricaria aurea. Phytochemistry 51: 551- 544, 1999.
Koster R, Anderson M, DeBeer E. Acetic acid analgesic screening. Federation Proceedings 18: 418-420, 1959.
Clark S, Follenfant R, Smith T. Evaluation of opioid-induced antinociceptive eff ects in anaesthetized and conscious animals. British Journal of Pharmacology 95: 275-283, 1988.
Pizziketti R, Pressman N, Geller E et al. Rat cold water tail fl ick: a novel analgesic test that distinguishes opioid agonists from mixed antagonists. European Journal of Pharmacology 119: 23-29, 1985
Janssen P.A, Niemegeers C.J, Dony J.G. Th e inhibitory eff ect of fentanyl and other morphine-like analgesics on the warm water induced tail withdrawal refl ex in rats. Arzneimittel Forschung/ Drug Research 6: 502-507, 1963.
Bianchi C, Franceschini J. Experimental observation on Haff ner’s method for testing analgesic drugs. British Journal of Pharmacology 9: 280-284, 1954.
Shibata M, Ohkubo T, Takahashi H et al. Modifi ed formalin test; characteristic biphasic pain response, Pain 38: 347-352, 1989.
Kesmati M, Barfi nejad N, Moghadam H. Eff ect of Matricaria recutita on acute pain in the presence and absence of sex hormones. Journal of research in medical sciences, 12: 190- 197, 2007
Islam AK, Cooper ML, Bodnar RJ. Interactions among aging, gender, and gonadectomy eff ects upon morphine antinociception in rats. Physiology & Behavior; 54: 45-53, 1993.
Viola H, Wasowski C, Levi de SM et al. Apigenin, a component of Matricaria recutita fl owers, is a central benzodiazepine receptors-ligand with anxiolytic eff ects. Planta Med, 61: 213- 216, 1995.
Medina JH, Paladini AC, Wolfman C et al. Chrysin (5,7-di- OH-fl avone), a naturally-occurring ligand for benzodiazepine receptors, with anticonvulsant properties. Biochemical Pharmacology, 40: 2227-2231, 1990.
Tatli II, Akdemir ZS, Yesilada E et al. Anti-infl ammatory and antinociceptive potential of major phenolics from Verbascum salviifolium Boiss. Z Naturforsch C. 63: 196-202, 2008
Qnais E, Abu-Safi eh K, Abu-Dieyeh M et al. Antinociceptive Eff ect of two fl avonoids from Aloysia triphylla L. Jordan Journal of Biological Sciences, 2: 167-170, 2009.
Yao CY, Wang J, Dong D et al. Laetispicine, an amide alkaloid from Piper laetispicum, presents antidepressant and antinociceptive eff ects in mice. Phytomedicine, 16: 823-9, 2009.
de Lima AB, Santana MB, Cardoso AS et al. Antinociceptive activity of 1-nitro-2-phenylethane, the main component of Aniba canelilla essential oil. Phytomedicine 16: 555-559, 2009.
Tjolsen A, Berge O, Hunskaar S et al. Th e formalin test: an evaluation of the method. Pain 51: 5-17, 1992.
Capone, F., Aloisi, A. Refi nement of pain evaluation techniques. Th e formalin test. Ann 1st super Sanita, 40: 223-229, 2004.
Santos A, Filho V, Niero R et al. Analgesic eff ects of callus culture extracts from selected species of Phyllantus in mice. Journal of Pharmacy and Pharmacology, 46: 755-759, 1994.
Choi S, Lee J, Suh H. Antinociceptive profi les of aspirin and acetaminophen in formalin, substance P and glutamate pain models. Brain Research, 921: 233-239, 2001.