Comparative analysis of DNA extracted from fish species preserved in formalin in two different periods

DNA was extracted from 2 cyprinid fishes kept under formalin preservation in 2 different time durations, and the quality and quantity were analyzed. The tissue samples from specimens preserved for shorter duration yielded higher quantity of DNA compared to long-term preserved tissues. ANOVA (one way) on the quantity of the DNA from the samples of 2 periods showed that the genetic material obtained from the recently preserved samples was highly significant (P > 0.001). The quality of the genetic material irrespective of the period of preservation was found to be the same. The purpose of the study was to check the viability of extracting genomic DNA from formalin preserved fish specimens and to estimate the quality and quantity.

Comparative analysis of DNA extracted from fish species preserved in formalin in two different periods

DNA was extracted from 2 cyprinid fishes kept under formalin preservation in 2 different time durations, and the quality and quantity were analyzed. The tissue samples from specimens preserved for shorter duration yielded higher quantity of DNA compared to long-term preserved tissues. ANOVA (one way) on the quantity of the DNA from the samples of 2 periods showed that the genetic material obtained from the recently preserved samples was highly significant (P > 0.001). The quality of the genetic material irrespective of the period of preservation was found to be the same. The purpose of the study was to check the viability of extracting genomic DNA from formalin preserved fish specimens and to estimate the quality and quantity.

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  • Wandeler P, Hoeck PEA, Keller LF. Back to the future: Museum specimens in population genetics. Tren Ecol Evol 22: 12, 634- 642, 2007.
  • Chakraborty A, Sakai M, Iwatsuki Y. Museum fi sh specimens and molecular taxonomy: A comparative study on DNA extraction protocols and preservation techniques. J Appl Ichthyol 22: 160-166, 2006.
  • Stuart BL, Dugan KA, Allard MW et al. Extraction of nuclear DNA from bone of skeletonized and fl uid-preserved museum specimens. Syst Biodiv 4: 2, 133-136. 2006. 4. Paabo S. Ancient DNA: extraction, characterization, molecular cloning, and enzymatic amplifi cation. Proc Nat Acad Sci USA 86: 1939-1943, 1989.
  • Fang S, Wan Q, Fujihara N. Formalin removal from archival tissue by critical point drying. Biotechniques 33: 604-611, 2002.
  • Koshiba M, Ogawa K, Hamazaki S et al. Th e eff ect of formalin fi xation on DNA and the extraction of high-molecular-weight DNA from fi xed and embedded tissues. Pathol Res Pract 89: 66-72, 1993.
  • Goetz SE, Hamilton SR, Vogelstein B. Purifi cation of DNA from formaldehyde fi xed and paraffi n embedded human tissue. Biochem Biophy Res Comm. 130: 118-126, 1985. 8. Debeau L, Chandler LA, Gralow JR et al. Southern blot Analysis of DNA extracted from formalin-fi xed pathology specimens. Cancer Res 46: 2964-2969, 1986.
  • Houde P, Braun MJ. Museum collections as a source of DNA for studies of avian phylogeny. Auk 105: 773-76, 1988.
  • Nielsen EE, Hansen MM, Loeschcke V. Analysis of DNA from old scale samples: technical aspects, applications and perspectives for conservation. Hereditas 130: 265-276, 1999.
  • Shiozawa DK, Kudo J, Evan RP et al. DNA extraction from preserved Trout Tissues. Great Basin Naturalist 53: 29-34, 1992.
  • Shedlock AM, Haygood MG, Pietsch TW et al. Enhanced DNA extraction and PCR amplifi cation of mitochondrial genes from formalin-fi xed museum specimens. Biotechniques 22: 394- 400, 1997.
  • Wirgin I, Maceda L, Stabile J et al. An evaluation of introgression of Atlantic coast striped bass mitochondrial DNA in a Gulf of Mexico population using formalin-preserved museum collections. Mol Ecol 6: 907-916, 1997.
  • Nishiguchi MK, Doukakis P, Egan M et al. DNA isolation Protocols. In: DeSalle R, Wheeler W, Giribet G. ed. Techniques in Molecular Systematics and Evolution Birkhäuser, Basel, Germany; 2002: pp. 250-287.
  • Wasko AP, Martins C, Oliveira C et al. Nondestructive genetic sampling in fi sh. An improved method for DNA extraction from fi sh fi ns and scales. Hereditas 138: 161-165, 2003.
  • Ferrara GB, Murgia B, Parodi AM et al. Th e assessment of DNA from marine organisms via a modifi ed salting out protocol. Cell Mole Biol Lett 11: 155-160, 2006.
Turkish Journal of Biology-Cover
  • ISSN: 1300-0152
  • Yayın Aralığı: Yılda 6 Sayı
  • Yayıncı: TÜBİTAK
Sayıdaki Diğer Makaleler

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